LOCUS       sequence-189921-        4773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF540 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF540
FEATURES             Location/Qualifiers
     source          1..4773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2084..2183)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2199..2216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2199..2215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2208..2230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2279..2365
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2457..2484
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2643..2660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2814..3402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2894..2913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3495..4286)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     4717..4733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4717..4733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4738..4757
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4738..4756
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttctta taatgccaac tttgtacaag aaagttggca ccatggccca tgcattggtg
      121 acgttcaggg atgtggctat agacttctct cagaaggaat gggagtgcct ggacactacc
      181 cagaggaaat tgtacagaga tgtgatgttg gagaattata ataacttggt ctcactggga
      241 tattctggct caaagccaga tgtgattacc ttactggagc aagggaaaga gccctgcgtg
      301 gtggcgaggg atgtgacagg aagacagtgc cccggtttgt tatccaggca taagaccaag
      361 aaattatctt cagaaaagga cattcatgaa atcagtttat ccaaagagag tataatagaa
      421 aaaagtaaaa ctcttcgtct gaaaggatcc atttttagaa atgagtggca gaacaaaagt
      481 gagtttgagg gtcaacaggg acttaaagaa agatctatca gtcaaaagaa aatcgtctct
      541 aaaaaaatgt caactgatag aaaacgtccc tcttttactc tgaatcagag aattcacaat
      601 agtgagaaaa gctgtgactc acacttggtt caacatggga aaatagattc tgatgtgaaa
      661 catgattgta aagaatgtgg gagtactttt aataatgtct atcagcttac tctccatcag
      721 aaaattcata ctggtgaaaa atcctgtaaa tgtgagaaat gtgggaaagt ttttagtcat
      781 agctatcaac ttactctgca tcagagattt catactggtg agaaacccta tgaatgtcaa
      841 gaatgtggga agacctttac tctttaccca caacttaatc gacatcagaa aattcacact
      901 ggtaaaaaac cctatatgtg taagaaatgt gataagggtt tttttagtag attagaactt
      961 actcaacata aaagaattca tactggtaag aaatcttatg aatgtaaaga atgtggaaaa
     1021 gtttttcaac ttattttcta ctttaaagaa catgagagaa ttcatacagg taagaaaccc
     1081 tatgaatgta aggagtgtgg gaaagctttt agtgtatgcg gacaacttac ccgtcatcag
     1141 aaaattcata ctggtgtaaa accctacgaa tgtaaggaat gtggaaagac ctttagactt
     1201 agtttttacc ttactgaaca cagaagaact catgcaggta agaaacctta tgaatgtaag
     1261 gagtgtggga aatcatttaa tgtgcgtgga cagcttaatc ggcataaaac aatccatact
     1321 ggtataaaac cttttgcatg taaggtgtgt gagaaggctt ttagttatag tggtgacctc
     1381 agagtacatt ctagaattca tactggagag aaaccatatg aatgtaagga atgcgggaaa
     1441 gcctttatgc ttcgttcagt ccttactgaa catcagagac ttcatactgg tgtgaagccc
     1501 tacgaatgta aggaatgtgg gaagaccttt cgagttcgtt ctcaaattag tctacataag
     1561 aaaattcata ctgatgtgaa gccctacaaa tgtgtacgat gtgggaagac ctttagattt
     1621 ggtttctacc ttactgaaca ccagagaatt cacactggtg aaaagcccta taaatgtaaa
     1681 gaatgtggaa aggcctttat tcgtagaggg aatcttaaag aacatctgaa aattcattct
     1741 ggtttaaaac cctatgactg taaagaatgt gggaagtcct ttagtcggcg tgggcagttc
     1801 actgaacatc agaaaattca tacgggtgta aaaccataca aatgtaaaga atgtgggaag
     1861 gcctttagtc gtagtgtaga ccttagaata catcaaagaa ttcatactgg tgagaaaccc
     1921 tatgagtgta aacaatgtgg gaaggccttt agacttaatt cacaccttac tgaacatcag
     1981 agaattcaca ctggtgagaa accctatgag tgtaaggtat gtagaaaggc ctttagacaa
     2041 tattcacatc tttatcaaca tcagaaaact cataatgtaa ttttgccaac tttcttgtac
     2101 aaagttggca ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc
     2161 agtcaaaata aaatcattat ttggggcccg agcttaagac tggccgtcgt tttacaacgt
     2221 cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca
     2281 aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2341 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg
     2401 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa
     2461 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct agcggtacgc
     2521 gtattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2581 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2761 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3001 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3241 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3421 tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga ttttggtcat
     3481 gggtggctcg agggttattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
     3541 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
     3601 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
     3661 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
     3721 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
     3781 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
     3841 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
     3901 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     3961 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     4021 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     4081 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     4141 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     4201 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     4261 acttcggcga tcaccgcttc cctcataatg tttaactttg ttttagggcg actgccctgc
     4321 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     4381 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa
     4441 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     4501 cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt
     4561 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag
     4621 gcatttctgt cctggctggt ctagaggagg catgatgata tatttttatc ttgtgcaatg
     4681 taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat gaccatgtaa
     4741 tacgactcac tataggggat atcagctgga tgg
//
