LOCUS       sequence-189930-        5514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF112 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF112
FEATURES             Location/Qualifiers
     source          1..5514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2825..2924)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2941..2960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2942..2960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2965..2981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2965..2981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3412..4203
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4296..4884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4785..4804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5038..5055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5214..5241)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5333..5419)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5468..5490
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5482..5499
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5483..5499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggtgac attcaaggat
      121 gttgctgtgg tcttcactga ggaggagctg gggctgctgg actctgtcca gaggaagctg
      181 taccgagatg tgatgctgga gaacttcagg aacctgctct tagtagcaca tcagcccttc
      241 aagccagacc taatatccca gctggagaga gaagaaaagc ttttgatggt ggagacagaa
      301 accccaaggg atggatgttc aggaaggaag aatcaacaaa agatggagag tattcaggaa
      361 gtaacagtaa gctacttttc ccccaaagag ctttcctccc gtcagacctg gcaacaaagt
      421 gcaggtgggt taatcaggtg tcaagatttc ctgaaagttt ttcaagggaa gaattctcag
      481 ttgcaagaac aaggtaattc cctcggccag gtttgggcag gaataccagt tcagatttct
      541 gaagataaga actatatatt gactcatata gggaatggct ccaattatat aaaaagtcaa
      601 gggtatccat cttggagggc acatcattct tggaggaaaa tgtatctgaa agagtcacat
      661 aattatcagt gtagatgtca gcaaatttcc atgaaaaatc atttctgtaa gtgtgacagt
      721 gtcagttggc tctcacatca caatgataaa ctggaagtac acagaaaaga aaactacagc
      781 tgccatgact gtggagaaga tatcatgaag gtatcattac ttaatcagga gtcaattcaa
      841 acagaggaga agccctatcc atgtactggg tatagaaaag ccttcagtaa tgactccagc
      901 tctgaagttc atcagcagtt ccacttggaa gggaagccct atacatacag ttcatgtgga
      961 aagggctgta attatagttc acttcttcat attcatcaaa atattgagag agaagatgat
     1021 attgagaatt cacatctgaa atcctatcag agagtgcata cagaggagaa accatgcaaa
     1081 tgtggtgaat atggtgagaa cttcaatcac tgttcccctc ttaacactta tgaacttatc
     1141 cacacaggtg agatgtccta taggcacaac atttatgaga aagccttcag tcatagctta
     1201 gaccttaata gtatttttag ggtccatact agggatgaac cccatgaata tgaggaaaat
     1261 gagaatgtct ttaatcagag ttcatgtctt caagtccatc aaaaaatcca cactgaagag
     1321 aaactataca cagatataga gtatggaaag agtttcattt gtagttcaaa tcttgacatt
     1381 cagcataggg ttcatatgga agagaattca tataattctc aggagtgtgg taatggcttc
     1441 agtctggcct cacattttca ggaccttcag atagtccaca ctaaggaaca accatataaa
     1501 cgctatgtgt gtagtaacag cttcagccat aatttacatc ttcaaggtca tccaaaaatt
     1561 cacattggag agaaaccacg taaggagcat gggaatggct tcaactggag ctcaaaactt
     1621 aaagatcatc agagagtcca cactggacag aagccataca aatgcaatat atgcggcaaa
     1681 ggtttcaatc atagatcagt tctgaatgtt catcagagag tccacaccgg agagaaacct
     1741 tataaatgcg aggaatgtga taagggattc agtcggagtt catatcttca agcccatcag
     1801 agagtccaca ctggagaaaa accttataaa tgtgaggaat gtgggaaggg gttcagtcga
     1861 aattcatacc ttcaaggcca tcagagagtt cacactggag aaaaaccata caagtgtgag
     1921 gagtgtggga agggcttcag tcggagttca caccttcaag gccatcagag agtccacact
     1981 ggagaaaaac cattcaaatg tgaggaatgt gggaaggggt tcagttggag ctttaatctc
     2041 caaattcatc agagggttca cacaggagaa aaaccctata aatgtgaaga atgtggtaaa
     2101 ggcttcagta aggcctcaac acttttggcc catcagaggg tccacacggg ggagaagcca
     2161 taccaatgtg atgagtgtgg taagagtttc agtcagagat cataccttca gagtcatcag
     2221 agtgtccatt ctggagaaag accatatata tgtgaggtat gtggaaaggg cttcagtcag
     2281 agagcatatc ttcaaggtca tcagagagtc cacactagag tgaaaccgta taaatgtgag
     2341 atgtgtggga agggctttag tcagagttcg cgccttgaag cacatcggag ggttcacaca
     2401 ggagggaaac catacaaatg tgcggtgtgt acaaagggtt tcagtgagag ttcacgcctt
     2461 caagcacacc aaagggttca tgtggaaggg agaccctata aatgtgaaca gtgtggtaag
     2521 ggtttcagtg ggtattcaag tcttcaagcc catcacagag tccacacagg agagaaacca
     2581 tacaaatgtg aggtatgtgg aaagggcttc agtcagagat caaatcttca ggctcaccag
     2641 agagtccaca caggagagaa accatacaaa tgtgatgcat gtggtaaggg tttccgttgg
     2701 agctcaggtc ttctcattca tcaaagagtc catagtagtg ataaattcta taaaagcgaa
     2761 gactatggta aggactaccc ttcatcagag aatctacaca gaaatgaaga ttctgttttg
     2821 tttttgccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2881 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     2941 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     3001 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
     3061 agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg
     3121 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     3181 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     3241 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     3301 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     3361 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa
     3421 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     3481 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     3541 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     3601 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     3661 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     3721 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     3781 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     3841 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     3901 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     3961 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     4021 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     4081 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     4141 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     4201 taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     4261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5041 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5101 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5161 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     5221 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     5281 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     5341 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     5401 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     5461 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
