LOCUS       sequence-189948-        5010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF624 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF624
FEATURES             Location/Qualifiers
     source          1..5010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2321..2420)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2437..2456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2438..2456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2461..2477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2461..2477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2908..3699
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3792..4380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4281..4300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4534..4551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4710..4737)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4829..4915)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4964..4986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4978..4995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4979..4995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggagcc caaacctgca
      121 accaagaagg ctacacgaac aaaggctatt tctgaagatt tatcacagga ggccatacta
      181 gagaaactta cagagaatgg tctgtgggat tccagaatgg aagggttatg gaaatggaat
      241 gataggatat tgagattaca aaataatcag gagaatcatt tgagtcaaag aataattcca
      301 ctcaagaaga ctcccactag tcaaagaggc tttagatttg aatctattct tattccagaa
      361 ccaggcattg ccacagaaga gcttcacagc agatgccaaa cacaagagga aaatttcaca
      421 gagaatttaa atttgattac agatacccat ttggggaaga taatttgcaa ggagatgaaa
      481 ggcagcaaag ccataaggca gacttcagaa ctaactttgg ggaaaaaatc caataataag
      541 gaaaaaccct acaaatgtag tacatgcgaa aaggcctttc attatagatc attgctcatt
      601 caacatcaaa gaactcatac taaagaaaaa ccttatgaat gtaatgaatg tgggaaaaca
      661 ttcagccagc cttcatatct cagtcagcac aaaaaaatcc acactggaga aaaaccctat
      721 aaatgtaatg aatgtggaaa ggccttcatt gcttcttcat cacttatggt acatcagaga
      781 attcacacta aagagaaacc ttatcagtgt aatgtgtgtg ggaaatcttt tagccagtgt
      841 gcccgtctta atcagcacca gagaattcaa actggagaga aaccctataa atgtagtgaa
      901 tgcgggaaag cttttagtga taaatcaaaa cttgcaagac atcaggaaac tcacaatggt
      961 gagaaaccct acaaatgtga tgattgtggg aaagccttta ggaacaagtc atatcttagt
     1021 gtacatcaga agacccacac tgaagagaaa ccatatcagt gcaacgagtg tgggaagtct
     1081 tttaagaata ccacaatttt taatgtgcat cagaggattc atactggaga gaaacctttt
     1141 agatgtaacg aatgtggaaa agcctataga agtaattcaa gccttatcgt acatataaga
     1201 actcacactg gggaaaaacc ctatgaatgt aatgaatgtg ggaaagcatt caaccgcatc
     1261 gcaaatttca cagaacatca gcgaattcac acaggagaaa aaccctataa atgtaatgaa
     1321 tgtgggaaag cattcattaa ttattcatgc cttactgtac accacagaat gcatacagga
     1381 gagaaacctt ataaatgtac tgaatgtgga aaggccttca tgcgttcttc ttctctaatt
     1441 atacatcagc gtattcatac tgaagagaaa ccttatctgt gcaatgaatg tggggagtct
     1501 ttcagaataa aatcacactt aactgtacat cagagaattc acactggaga gaaaccatat
     1561 aaatgtactg actgcgagag ggcattcacc aaaatggtaa atctcaagga gcatcagaaa
     1621 attcatactg gagtgaaacc ctataaatgt tatgactgtg gaaagtcctt taggactaaa
     1681 tcatacctta ttgtacatca gaggacccat actggagaaa aaccatataa atgtaatgaa
     1741 tgtgagaaag ccttcactaa tacatcacag cttaccgtgc accaacgaag gcatactgga
     1801 gagaagccct ataaatgcaa tgaatgtgga aaggttttca caagtaactc aggctttaat
     1861 acacatcaaa gaacacatac tggagagaaa ccatttaaat gtaatgactg tgggaaagca
     1921 tttagccaga tggtacatgt cacagaacat cagaaaatcc atagtggaga gaagccctat
     1981 aagtgtgatg tctgtggaaa agccttcagg aggggttctt accttacagt gcattggaga
     2041 acacacactg gagaaaaacc ctacacctgt aaggaatgtg gaaagggttg tattactcta
     2101 tcacagctaa ccctacatca gagaattcat actggggaga ggccctataa atgtgaagaa
     2161 tgtggaaaag ccttcagaac taactcagac tttactgtac acttgaggat gcatactgga
     2221 gaaaaaccct ataaatgtaa tgaatgtgga aaagccttca ggagtagttc gagccttact
     2281 gtacatcaaa gaatacatca aagagaaact cagttaatat tgccaacttt cttgtacaaa
     2341 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     2401 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2461 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2521 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     2581 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     2641 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     2701 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     2761 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     2821 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2881 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     2941 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     3001 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     3061 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     3121 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     3181 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     3241 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     3301 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     3361 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     3421 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     3481 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     3541 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     3601 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     3661 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     3721 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3781 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3841 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3901 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3961 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4021 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4081 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4141 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4201 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4261 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4321 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4381 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4441 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4501 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4561 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4621 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4681 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4741 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4801 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4861 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4921 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4981 aaaacgacgg ccagtcttaa gctcgggccc
//
