LOCUS       sequence-189950-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF786 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF786
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2450..2549)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2566..2585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2567..2585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2590..2606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2590..2606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3037..3828
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3921..4509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4410..4429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4663..4680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4839..4866)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4958..5044)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5093..5115
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5107..5124
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5108..5124
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggcgga gccgcctcgg
      121 ctacctctga cttttgagga tgttgctatt tatttctccg agcaagaatg gcaggatcta
      181 gaggcatggc agaaggaact ttacaagcat gtgatgagaa gcaattatga gactctcgtc
      241 tctctagatg atggacttcc aaaaccagaa ctaatatcct ggattgaaca cgggggagag
      301 cccttcagga aatggagaga atcacagaaa tcaggaaaca taatttgctc ctctgttgat
      361 atgcattttg atccaggttt tgaggaacag ctgttttggg gaagccagca ggctatgaat
      421 tcaggaaaaa ctaaaagcca tttccaatta gatcctgaaa gccagtgttc ctttggatcc
      481 tttgtttcct tcaggcctga ccaaggcatc accctcggga gcccacagag acacgacgcc
      541 agggctcctc caccactagc ctgcggcccc agtgaatcta ccctaaaaga aggaatccca
      601 ggtcccagaa atctggatct tcctggtttg tgggacgtcc ccgcctggga gagcacccag
      661 cacccttggc ctgtctgcgg ggaaagctgt tgggagaaca accatttagt aatgcaccag
      721 agaggccact caaaggaccg gacacgtagg gcctgggaga aattcaacaa gagggcggag
      781 acgcagatgc cgtggagcag ccctcgggta cagaggcact tccggtgtgg cgtgtgcggt
      841 aagagcttcc gccggaagct gtgtctgctg cgccatctgg cggcccacac ggggaggggc
      901 cccttccgga acgctgacgg tgaaatgtgc ttccgacacg agctgaccca tcccagccac
      961 cgcctcccgc agcaggggga gaagcctgcc cagtgcaccc catgcggcaa gcgctccctc
     1021 ccagtggaca gcacgcaggc tcgccggtgc cagcacagcc gggaggggcc ggcctcttgg
     1081 agagaaggcc gcggggcctc cagcagtgtg cactcgggac agaaaccagg ctcgcgcctg
     1141 ccccaggagg ggaacagcca ccaggaaggg gacacggagg cgctgcagca tggcgcagag
     1201 gggccctgct cctgctcgga gtgtggcgag cgctccccta tgagcgccag gctcgccagc
     1261 ccctgcaggg cgcatactgg agaaaagccc ttccagtgtg cgcattgcac caagcgcttc
     1321 cgcctgcgcc gcctgctgca ggtccaccag cacgcgcacg gtggggagag accgttctcc
     1381 tgcaggaagt gtggcaaggg cttcgccaag cagtgtaaac tcacggagca cattcgagtc
     1441 cacagcggag agaagccttt ccggtgtgcc aagtgtggca ggaacttccg tcagagggga
     1501 cagctgctgc ggcaccagcg gctgcacacg gacgagaagc cctttcagtg cccagagtgt
     1561 gggctgagct tccgcctgga gagcatgctg agagcccacc ggctccggca cggtggggag
     1621 aggccgttct cctgtagcga gtgtggcaga ggcttcaccc accagtgcaa gctccgtgag
     1681 cacctgagag tgcacagcgg ggagaggccc ttccagtgcc tgaagtgcga caagcgcttc
     1741 cgcctgaagg gcatcctgaa ggcccaccag cacacgcaca gcaaggagag gccgttctcg
     1801 tgcggggagt gtggcaaggg cttcaccaga caatccaagc tcacggagca cttgcgcgtg
     1861 cacagcgggg agagaccctt ccagtgccca gagtgcaaca ggagcttccg cctgaagggg
     1921 cagctgctca gccatcagcg cctgcacacg ggagagaggc ccttccagtg tccggagtgc
     1981 gacaagcgct atcgcgtgaa ggccgacatg aaggcccacc agctgctgca cagcggggag
     2041 atgcctttct cctgtgagtg cggcaagggc tttgtgaaac actcaaagct catcgagcac
     2101 atcagaacgc acacgggaga gaagcctttt cagtgtccca agtgtgacaa gagtttccgc
     2161 ctgaaggcgc agctgctcag ccatcagggc ctgcacacag gggagaggcc ttttcactgc
     2221 cctgagtgtg acaagaactt ccgggaaagg ggacacatgc tgaggcacca gcgcatccac
     2281 aggcccgaga ggccctttgc ctgtggcgat tgtgggaagg gcttcattta caagtctaag
     2341 cttgcggagc acatcagagt acacacaaaa tcctgtcctg ctccaaatga actggacatt
     2401 aagaaaaggc tcagccaact gtttgcaatg atagaggccg attggagttt gccaactttc
     2461 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2521 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2581 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2641 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     2701 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     2761 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     2821 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2881 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     2941 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3001 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     3061 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     3121 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     3181 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     3241 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     3301 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     3361 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3421 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     3481 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     3541 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     3601 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     3661 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     3721 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3781 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     3841 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3901 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3961 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4021 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4081 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4141 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4321 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4381 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4501 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4561 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4621 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4681 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4741 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4801 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4861 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4921 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4981 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     5041 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     5101 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
