LOCUS       sequence-189966-       11514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and 
            Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the 
            control of TEF1/TEF2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers 
            CE
  TITLE     A squalene synthase protein degradation method for improved 
            sesquiterpene production in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. 
            Epub 2016 Dec 8.
  PUBMED    27939849
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-01"; volume: "39:209-219. doi"; pages: " 
            10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8"
COMMENT     Sequence Label: pJT1
FEATURES             Location/Qualifiers
     source          1..11514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2319..2732
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(4828..4848)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4828..4846)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4867..4883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4867..4883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4880..4902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4891..4907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4915..4921,4922..4939,4940..4945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4960..4981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5098..5115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5269..5857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5349..5368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6028..6819,6820..6888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6651..6670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6889..6993)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7020..8362
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7983..8030)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8403..8421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8459..8481)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8581..8600
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(9094..10188)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(10189..10596)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10897..11352)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11034..11055)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11246..11265
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11478..11500
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11492..11509
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11493..11509
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccctgcagac tttgttatgt
       61 agagtttttt tagctaccta tattccacca taacatcaat catgcggttg ctggtgtatt
      121 taccaataat gtttaatgta tatatatata tatatatatg gggccgtata cttacatata
      181 gtagatgtca agcgtaggcg cttcccctgc cggctgtgag ggcgccataa ccaaggtatc
      241 tatagaccgc caatcagcaa actacctccg tacattcatg ttgcacccac acatttatac
      301 acccagaccg cgacaaatta cccataaggt tgtttgtgac ggcgtcgtac aagagaacgt
      361 gggaactttt taggctcacc aaaaaagaaa gaaaaaatac gagttgctga cagaagcctc
      421 aagaaaaaaa aaattcttct tcgactatgc tggaggcaga gatgatcgag ccggtagtta
      481 actatatata gctaaattgg ttccatcacc ttcttttctg gtgtcgctcc ttctagtgct
      541 atttctggct tttcctattt tttttttttc catttttctt tctctctttc taatatataa
      601 attctcttgc attttctatt tttctctcta tctattctac ttgtttattc ccttcaaggt
      661 ttttttttaa ggagtacttg tttttagaat atacggtcaa cgaactataa ttaactaaac
      721 atggcttcag aaaaagaaat taggagagag agattcttga acgttttccc taaattagta
      781 gaggaattga acgcatcgct tttggcttac ggtatgccta aggaagcatg tgactggtat
      841 gcccactcat tgaactacaa cactccaggc ggtaagttaa atagaggttt gtccgttgtg
      901 gacacgtatg ctattctctc caacaagacc gttgaacaat tggggcaaga agaatacgaa
      961 aaggttgcta ttctaggttg gtgcattgag ttgttgcagg cttacttctt ggtcgccgat
     1021 gatatgatgg acaagtccat taccagaaga ggccaaccat gttggtacaa ggttcctgaa
     1081 gttggggaaa ttgccatcaa tgacgcattc atgttagagg ctgctatcta caagcttttg
     1141 aaatctcact tcagaaacga aaaatactac atagatatca ccgaattgtt ccatgaagtc
     1201 accttccaaa ccgaattggg ccaattgatg gacttaatca ctgcacctga agacaaagtc
     1261 gacttgagta agttctccct aaagaagcac tccttcatag ttactttcaa gactgcttac
     1321 tattctttct acttgcctgt cgcattggct atgtacgttg ccggtatcac agatgaaaag
     1381 gatttgaaac aagccagaga tgtcttgatt ccattgggtg aatatttcca aattcaagat
     1441 gactacttag actgcttcgg taccccagaa cagatcggta agatcggtac agatatccaa
     1501 gataacaaat gttcttgggt aatcaacaag gcattagaac ttgcttccgc agaacaaaga
     1561 aagactttag acgaaaatta cggtaagaag gactcagtcg cagaagccaa atgcaaaaag
     1621 attttcaatg acttgaaaat cgaccagtta taccacgaat atgaagagtc tgttgccaag
     1681 gatttgaagg ccaagatctc ccaagtcgac gagtctcgtg gcttcaaagc cgacgtctta
     1741 actgcgtttt tgaacaaagt ttacaagaga agcaaataat agtgagaagt tttgttagaa
     1801 aataaatcat tttttaattg agcattctta ttcctatttt atttaaatag ttttatgtat
     1861 tgttagctac atacaacagt ttaaatcaaa ttttcttttt cccaagtcca aaatggaggt
     1921 ttattttgat gacccgcatg cgattatgtt ttgaaagtat aagactacat acatgtacat
     1981 atatttaaac atgtaaaccc gtccattata ttgcttactt tcttcttttt tgccgttttg
     2041 acttggacct ctggtttgct atttccttac aatctttgct acaatggatc ccgggttgac
     2101 agcctagaca tcaatagtca tacaacagaa agcgaccacc caactttggc tgataatagc
     2161 gtataaacaa tgcatacttt gtacgttcaa aatacaatgc agtagatata tttatgcata
     2221 ttacatataa tacatatcac ataggaagca acaggcgcgt tggactttta attttcgagg
     2281 accgcgaatc cttacatcac acccaatccc ccacaagtga tcccccacac accatagctt
     2341 caaaatgttt ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt
     2401 accacttcaa aacacccaag cacagcatac taaatttccc ctctttcttc ctctagggtg
     2461 tcgttaatta cccgtactaa aggtttggaa aagaaaaaag agaccgcctc gtttcttttt
     2521 cttcgtcgaa aaaggcaata aaaattttta tcacgtttct ttttcttgaa aatttttttt
     2581 tttgattttt ttctctttcg atgacctccc attgatattt aagttaataa acggtcttca
     2641 atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta cttcttgctc
     2701 attagaaaga aagcatagca atctaatcta agttttaatt acaaaatggc taccgcagca
     2761 ggtcctatcg caactaacaa ctccccacaa aactccaacg cttacagaac tccaatcgct
     2821 ccttccgtac caattactca taaatggtct atagctgaag atttgacatg tatttccaat
     2881 cctagtaagc acaataaccc tcaaactggt tacagatcat tttctgacga attatacgtt
     2941 aagtacgaag aaaagttgga agatgttaga aaagcattaa gagaagttga agaaaaccct
     3001 ttggaaggtt tagttatgat agacgctttg caaagattgg gtatcgatta ccatttcaga
     3061 ggtgaaattg gtgcattctt gcaaaagcaa caaatcatat cttcaactcc agatggttac
     3121 cctgaacatg gtttgtacga agtttcaaca ttgtttagat tcttaagaca agaaggtcac
     3181 aatgttaccg ctgacgtctt taataacttc aaggataagg aaggtagatt cagatcagaa
     3241 ttgtcaacag atattagagg tttgatgtcc ttatacgaag caagtcaatt gagaatagaa
     3301 ggtgaagaca tcttagatca agctgctgat ttctccagtc aattgttagg tagatggaca
     3361 aaagatccta atcatcacga agccagattg gtttctaaca ctttaacaca tccataccac
     3421 aagtcattgg ctacctttat gggtcaaaaa ttgtcctaca tgaactgcaa gggtccaaac
     3481 tgggacggtg tcgataattt gcaagaatta gctaagatgg atttgactat cgtacaaagt
     3541 atccatcaaa aggaagtatt ccaagtttct caatggtgga aggatacagg tttagccaat
     3601 gaattgaaat tggctagaaa ccaaccattg aagtggtata tgtggcctat ggccgcttta
     3661 accgatccaa gattcagtga agaaagagtt gaattaacta agcctatttc ttttatatac
     3721 atcatagatg acatcttcga cgtctatggt accattgaag aattgacctt gtttactgat
     3781 gcagttaata gatgggaatt gtctgccgtc gaacaattac cagactacat gaaagtatgt
     3841 ttcaaggctt tgtacgatgt taccaacgaa atcgcataca aaatctataa aaagcatggt
     3901 caaaacccta ttgattcctt gcaaaagact tgggctagtt tgtgcaatgc atttttagtt
     3961 gaagcaaagt ggttcgcctc tggtcacttg ccaaatgcag aagaatactt aaagaacggt
     4021 atcatctctt caggtgttca tgttgtcttg gcccacatgt ttttcttgtt aggtgacggt
     4081 attacacaag aatcagttga tttggtagat gactatccag gtatttccac aagtatcgca
     4141 accattttga gattatctga tgacttgggt tcagccaaag atgaagacca agatggttat
     4201 gatggttctt acatcgaatg ttacatgaag gaacataagg gttccagtgt cgattcagcc
     4261 agagaagaag taataagaat gatctccgaa gcatggaaat gtttgaataa ggaatgctta
     4321 tcaccaaacc ctttttctga atcattcaga ataggttcct tgaatatggc tagaatgatc
     4381 cctatgatgt actcttacga tgacaaccat aacttgccaa ttttagaaga acacatgaag
     4441 gcaatgatct ataacacatc tttataatag tgagcggatt gagagcaaat cgttaagttc
     4501 aggtcaagta aaaattgatt tcgaaaacta atttctctta tacaatcctt tgattggacc
     4561 gtcatccttt cgaatataag attttgttaa gaatatttta gacagagatc tactttatat
     4621 ttaatatcta gatattacat aatttcctct ctaataaaat atcattaata aaataaaaat
     4681 gaagcgattt gattttgtgt tgtcaactta gtttgccgct atgcctcttg ggtaatgcta
     4741 ttattgaatc gaagggcttt attatattac cctttagctt attctgaggt ttctgtggcg
     4801 tccgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     4861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5041 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5701 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7021 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     7081 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     7141 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     7201 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     7261 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     7321 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     7381 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     7441 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     7501 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     7561 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     7621 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     7681 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     7741 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     7801 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     7861 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     7921 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
     7981 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     8041 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     8101 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     8161 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     8221 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     8281 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     8341 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
     8401 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     8461 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8521 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     8581 cggcatcaga gcagattgta ctgagagtgc accatatcga ctacgtcgtt aaggccgttt
     8641 ctgacagagt aaaattcttg agggaacttt caccattatg ggaaatggtt caagaaggta
     8701 ttgacttaaa ctccatcaaa tggtcaggtc attgagtgtt ttttatttgt tgtatttttt
     8761 tttttttaga gaaaatcctc caatatataa attaggaatc atagtttcat gattttctgt
     8821 tacacctaac tttttgtgtg gtgccctcct ccttgtcaat attaatgtta aagtgcaatt
     8881 ctttttcctt atcacgttga gccattagta tcaatttgct tacctgtatt cctttacatc
     8941 ctcctttttc tccttcttga taaatgtatg tagattgcgt atatagtttc gtctacccta
     9001 tgaacatatt ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt
     9061 tatgtacaaa tatcataaaa aaagagaatc tttttaagca aggattttct taacttcttc
     9121 ggcgacagca tcaccgactt cggtggtact gttggaacca cctaaatcac cagttctgat
     9181 acctgcatcc aaaacctttt taactgcatc ttcaatggcc ttaccttctt caggcaagtt
     9241 caatgacaat ttcaacatca ttgcagcaga caagatagtg gcgatagggt tgaccttatt
     9301 ctttggcaaa tctggagcag aaccgtggca tggttcgtac aaaccaaatg cggtgttctt
     9361 gtctggcaaa gaggccaagg acgcagatgg caacaaaccc aaggaacctg ggataacgga
     9421 ggcttcatcg gagatgatat caccaaacat gttgctggtg attataatac catttaggtg
     9481 ggttgggttc ttaactagga tcatggcggc agaatcaatc aattgatgtt gaaccttcaa
     9541 tgtagggaat tcgttcttga tggtttcctc cacagttttt ctccataatc ttgaagaggc
     9601 caaaacatta gctttatcca aggaccaaat aggcaatggt ggctcatgtt gtagggccat
     9661 gaaagcggcc attcttgtga ttctttgcac ttctggaacg gtgtattgtt cactatccca
     9721 agcgacacca tcaccatcgt cttcctttct cttaccaaag taaatacctc ccactaattc
     9781 tctgacaaca acgaagtcag tacctttagc aaattgtggc ttgattggag ataagtctaa
     9841 aagagagtcg gatgcaaagt tacatggtct taagttggcg tacaattgaa gttctttacg
     9901 gatttttagt aaaccttgtt caggtctaac actaccggta ccccatttag gaccacccac
     9961 agcacctaac aaaacggcat cagccttctt ggaggcttcc agcgcctcat ctggaagtgg
    10021 aacacctgta gcatcgatag cagcaccacc aattaaatga ttttcgaaat cgaacttgac
    10081 attggaacga acatcagaaa tagctttaag aaccttaatg gcttcggctg tgatttcttg
    10141 accaacgtgg tcacctggca aaacgacgat cttcttaggg gcagacatta gaatggtata
    10201 tccttgaaat atatatatat atattgctga aatgtaaaag gtaagaaaag ttagaaagta
    10261 agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata ttgtcaactt
    10321 caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat gaaaagccgg
    10381 ttccggcgct ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt
    10441 caggcgacct ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag
    10501 cgcccctgtg tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac
    10561 tcttgcatct tacgatacct gagtattccc acagttaact gcggtcaaga tatttcttga
    10621 atcaggcgcc ttagaccgct cggccaaaca accaattact tgttgagaaa tagagtataa
    10681 ttatcctata aatataacgt ttttgaacac acatgaacaa ggaagtacag gacaattgat
    10741 tttgaagaga atgtggattt tgatgtaatt gttgggattc catttttaat aaggcaataa
    10801 tattaggtat gtagatatac tagaagttct cctcgaccgg tcgatatgcg gtgtgaaata
    10861 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
    10921 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10981 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    11041 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    11101 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    11161 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    11221 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    11281 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    11341 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11401 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11461 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcg
//
