LOCUS       sequence-189969-        6833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP2.1.1
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1832..1849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1838..2062
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2194..2782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2274..2293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3111..3158
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3183..3202
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3390..4184)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3390..4184)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3501..3520)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4111..4130
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4219..4576)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4233..4368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4385..4576
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4533..4553
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4636..5427,5428..5496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5259..5278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5497..5601)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5628..6083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5715..5734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5925..5946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6095..6474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6475..6678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6629..6649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6723..6742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6723..6741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggttgctct ttttctatct tcctgctagc tcttttgtct tgcatcaccg tgccagtttc
       61 tgccgcggaa gtcaaaaaca ccagccaaac atacatggtg accaatgact gctcgaacag
      121 cagcattacc tggcagcttg agaacgcggt gcttcacgtt cctggatgcg tcccctgtga
      181 acgagacggc aacacatccc ggtgttggtt accagtcgcg cccaacgtag ccgtgagccg
      241 acctggtgcc ttaactgcgg gtttgcgatc gcacgtcgac accatcgtgg cgtctgcgac
      301 gctttgttct gccctctaca taggggatgt atgtggcgcg ataatgatag ctgcccaagt
      361 tgtcatcgtc tcgccgcagc atcaccagtt tgtccaggac tgcaattgtt ccatctaccc
      421 tggtcatgta tctggacatc gtatggcttg ggacatgatg atgaactggt cgccgacgac
      481 taccatggtt ctgggctatc tgatgcgcgt gcctgaagtg gtcttggaca tcatcgccga
      541 cggccactgg ggggtgatgt tcggcctggc ctacttctcc atgcaaggag cgtgggcaaa
      601 agtcgtagtc atcctcctac tatgcgctgg ggtcgacgcg tcgacgcatc ttattgcggg
      661 ctcagcaggc aacaccgtgc gcaggtttgc cggcgtgttt tcctcggggc cccagcagaa
      721 catccagctc attaatacta atggcagctg gcacatcaac cgcacagccc taaactgcaa
      781 tgactccctt cagactggct ttattgcctc gctgttctac accaccagct tcaatgcatc
      841 aggctgtcct gaacgcatgg cctcttgccg taacatcgag tcctttcgga ttgggtgggg
      901 caccttggag tatgatgaaa atgtcaccaa cgaggaggac atgaggcctt actgctggca
      961 ttacccgccc aaaccgtgtg gagtggttcc tgcccggtcc gtgtgtggcc cagtttactg
     1021 tttcaccccc tccccagtgg tggtgggcac aaccaacaaa ctcggtgtcc ccacgtacac
     1081 ttggggggag aatgagacag atgtcttcct cttaaacagc accaggcctc ctaggggagc
     1141 ctggtttgga tgcacatgga tgaacggcac cggattcacc aaaacatgcg gcgcaccgcc
     1201 gtgccgcatt aggcgggact tcaacaccag cgtggacttg ttgtgcccca cagactgctt
     1261 cagaaaacac cctgaggcaa catacagtaa atgcggggcc gggccctggc tgacgcccaa
     1321 gtgcctgatc gattacccct ataggctgtg gcattacccg tgcaccgtca actataccat
     1381 ctttaagata aggatgtttg tggggggaat cgagcacaga ctccaagcgg catgcaattt
     1441 cactcgtggg gaccgctgtg accttgacga cagggatagg agtcagctga gtccgctctt
     1501 acactctacc accgagtggg ccatcttgcc ctgcaccttt tcagacttgc cagccctctc
     1561 cacggggctc atccacctcc accagaacac cgttgacgtc cagtacctgt atggcctgtc
     1621 accggccatc acaaaatacg tcgtcaagtg ggagtgggta gtgctcctct tcttactact
     1681 ggcggatgcc agggtatgtg cctgtctatg gatgctcatc ctgttgggcc aagccgaagc
     1741 gtaatctaga aagtttaagc cgaattctgc agatatccag cacagtggcg gccgctcgag
     1801 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     1861 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1921 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1981 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2041 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagtggcgg taatacggtt
     2101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2821 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2881 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     2941 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     3001 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     3061 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     3121 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     3181 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     3241 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     3301 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     3361 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     3421 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     3481 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     3541 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     3601 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     3661 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     3721 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     3781 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     3841 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     3901 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     3961 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     4021 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     4081 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     4141 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     4201 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     4261 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     4321 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     4381 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     4441 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     4501 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     4561 gttctttccg cctcaggact cttccttttt caataaatca atctaaagta tatatgagta
     4621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     5641 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5701 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5761 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5821 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5881 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5941 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6001 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6061 caaaatatta acgcttacaa tttacgcgcg cgttgacatt gattattgac tagttattaa
     6121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     6181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     6361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     6481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     6541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     6601 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     6661 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
     6721 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta
     6781 ccgagctcgg atccactagt ccagtgtggt ggaattcggc ttgctcgagc cat
//
