LOCUS       sequence-189972-        6833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP2.1.2
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1836..1853)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1842..2066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2198..2786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2278..2297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3115..3162
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3187..3206
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3394..4188)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3394..4188)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3505..3524)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4115..4134
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4223..4580)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4237..4372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4389..4580
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4537..4557
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4640..5431,5432..5500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5263..5282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5501..5605)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5632..6087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5719..5738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5929..5950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6099..6478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6479..6682
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6633..6653
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6727..6746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6727..6745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccatgggttg ctctttttct atcttcctgc tagctctctt gtcctgcatc accgtgccag
       61 tttctgccgc ggaagtcaaa aacaccagcc aaacatacat ggtgaccaat gactgctcga
      121 acagcagcat tacctggcag cttgagaacg cggtgcttca cgttcctgga tgcgtcccct
      181 gtgaacgaga cggcaacaca tcccggtgtt ggttaccagt cgcgcccaac gtagccgtga
      241 gccgacctgg tgccttaact gcgggtttgc gatcgcacgt cgacaccatc gtggcgtctg
      301 cgacgctttg ttctgccctc tacatagggg atgtatgtgg cgcgataatg atagctgccc
      361 aagttgtcat cgtctcaccg cagcatcacc agtttgtcca ggactgcaat tgttccatct
      421 accctggtca tgtatctgga catcgtatgg cttgggacat gatgatgaac tggtcgccga
      481 cgactaccat ggttctgggc tatctgatgc gcgtgcctga agtggtcttg gacatcatcg
      541 ccgacggcca ctggggggtg atgttcggcc tggcctactt ctccatgcaa ggagcgtggg
      601 caaaagtcgt agtcatcctt ctactatgcg ctggggtcga cgcgtcgacg catcttattg
      661 cgggctcagc aggcaacact gtgcgcaggt ttgccggcgt gttttcctcg gggccccagc
      721 agaacatcca gctcattaat actaacggca gctggcacat caaccgcaca gccctaaact
      781 gcaatgactc ccttcagact ggctttattg cctcgctgtt ctacaccacc agcttcaatg
      841 catcaggctg tcctgaacgc atggcctctt gccgtaccat cgagtccttt cggattgggt
      901 ggggcacctt ggagtatgat gaaaatgtca ccaacgagga ggacatgagg ccttactgct
      961 ggcattaccc gcccaaaccg tgtggagtgg ttcctgcccg gtccgtgtgt ggcccagttt
     1021 actgtttcac cccctccccg gtggtggtgg gtacaaccaa caagcttggt gtccccacgt
     1081 acacttgggg ggaaaatgag acagatgtct tcctcttaaa cagcaccagg cctcctaggg
     1141 gagcctggtt tggatgcaca tggatgaaca gcaccggatt caccaaaaca tgcggcgcac
     1201 caccgtgccg cattaggcgg gacttcaaca ccagcgtgga cttactgtgc cccacagact
     1261 gcttcagaaa acacccagag gcaacataca gtaaatgcgg ggccgggcct tggctgacgc
     1321 ccaagtgcct gatcgattac ccctataggc tgtggcatta cccgtgcacc gtcaactata
     1381 ccatctttaa gataaggatg tttgtggggg gaatcgagca cagactccaa gcggcatgca
     1441 atttcactcg tggggaccgc tgtgaccttg acgacaggga taggagtcag ctgagcccac
     1501 tcttacactc caccaccgag tgggccatct taccctgcac ctattcagac ttgccagccc
     1561 tctccacggg gctcatccac ctccaccaga acaccgttga cgtccagtac ctgtatggcc
     1621 tgtcaccggc catcacaaaa tacgtcgtca agtgggagtg ggtagtgctc ctcttcttac
     1681 tactggcgga cgccagggta tgtgcctgcc tatggatgct catcctgttg ggccaagccg
     1741 aagcgtaatc tagaaagttt aagccgaatt ctgcagatat ccagcacagt ggcggccgct
     1801 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     1861 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1921 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1981 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2041 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac
     2101 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2161 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2221 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2401 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2521 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2581 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2641 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2701 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2821 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2881 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2941 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
     3001 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
     3061 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
     3121 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
     3181 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
     3241 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     3301 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     3361 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     3421 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     3481 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     3541 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     3601 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     3661 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     3721 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     3781 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     3841 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     3901 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     3961 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     4021 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     4081 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     4141 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     4201 tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact
     4261 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     4321 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     4381 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4441 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     4501 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     4561 gctggttctt tccgcctcag gactcttcct ttttcaataa atcaatctaa agtatatatg
     4621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4921 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5221 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     5641 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5701 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5761 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5821 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5881 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5941 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6001 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6061 ttaacaaaat attaacgctt acaatttacg cgcgcgttga cattgattat tgactagtta
     6121 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     6181 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     6241 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     6301 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     6361 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     6421 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     6481 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     6541 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     6601 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     6661 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     6721 cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt
     6781 ggtaccgagc tcggatccac tagtccagtg tggtggaatt cggcttgctc gag
//
