LOCUS       sequence-189974-        2602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of GFP spaghetti monster myc construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pAAV.CAG.GFPsm-myc.WPRE.SV40
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     388..410
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     472..491
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             536..565
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             569..598
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             602..631
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1163..1192
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1199..1228
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1241..1270
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1277..1306
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1520..1549
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1553..1582
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1586..1615
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1635..2223
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1688..1708)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2106..2117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2266..2387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2277..2296)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2331..2350
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      361 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      421 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
      481 tggttattgt gctgtctcat cattttggca aagaattgga tcggccgcca ccatggagca
      541 aaaactgata tcggaggaag acttggcaga gcagaaactg atttcggaag aggatcttgc
      601 cgaacaaaaa ctgatcagtg aggaagacct tgccggagtg tcgaagggtg aagagctgtt
      661 caccggcgtg gtgcccatcc tcgtcgagct ggacggagat gtcaatggac ataaattctc
      721 cgtacgggga gagggcgagg gcgacgcaac caacggtaaa ctgaccctga agttcatttg
      781 caccacgggt aaactgcccg taccctggcc caccctggtc acaaccttgg gtggcggagt
      841 tcagtgtttt tcgcggtatc cagatcacat gaagcagcat gactttttca agagcgctat
      901 gcccgagggt tacgttcaag agaggaccat cagtttcaag gacgacggca cctacaagac
      961 acgggctgag gtgaaattcg agggtgatac actcgtgaat cgtatcgagc tgaagggcat
     1021 agattttaag gaggatggca atatcctggg ccacaagttg gagtataact tcaacagtca
     1081 caatgtctat ataactgccg ataagcagaa gaatggcatt aaagccaatt tcaaaattcg
     1141 tcacaatgtc gaaggcggcg cagaacagaa gctcatcagt gaagaggatc tggccgcaga
     1201 gcagaaactc attagcgagg aggacttggg cggaggcggc gagcagaagc tgatatcgga
     1261 ggaagacctg gcggccgagc aaaagctcat ctcggaggag gatctcgcgg gaggagatgg
     1321 aagtgtccag ttggcggatc actaccagca gaatacgcca attggtgacg gccccgtcct
     1381 tctgccggat aaccactacc tctccaccca gtcggtgctg agcaaggacc cgaatgaaaa
     1441 acgtgaccac atggttcttc ttgagttcgt cacggctgcg ggcatcacgt tgggcatgga
     1501 cgaactgtac aagggcgctg aacagaaact tatctccgag gaggatctgg cggaacagaa
     1561 gctgatcagc gaagaggacc tggcggagca gaaacttatc agcgaagagg atctgtaaac
     1621 cggagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1681 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1741 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1801 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1861 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1921 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1981 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2041 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2101 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2161 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2221 cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg
     2281 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2341 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2401 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
     2461 acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag
     2521 tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc
     2581 tctctgcgcg ctcgctcgct ca
//
