LOCUS       sequence-189977-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Olson R, Huey-Tubman KE, Dulac C, Bjorkman PJ
  TITLE     Structure of a pheromone receptor-associated MHC molecule with an 
            open and empty groove.
  JOURNAL   PLoS Biol. 2005 Aug . 3(8):e257.
  PUBMED    16089503
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2005 Aug . 3(8):e257."
COMMENT     Sequence Label: pAcUW31-M10.5/human b2m
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    153..174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(189..194,195..212,213..219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    227..243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     232..254
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     251..267
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     251..267
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_recomb     1549..2376
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1549..1673
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1711..2316)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     polyA_signal    2382..2516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2406..2425)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2460..2479
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(3219..3328)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3476..3567
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3492..3509
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4481..4492
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4493..4510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     4522..5879
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(4538..5879)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7027..7044)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7027..7043)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7036..7058)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7256..7711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7343..7362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7553..7574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7728..7747)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7847..7869
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7907..7925)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7993..8097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8098..8166,8167..8958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8316..8335)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9129..9717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9618..9637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
       61 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      121 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      181 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      241 caatttcaca caggaaacag ctatgacatg attacgaatt tcgagcagtt cgttgacgcc
      301 ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg accagcggcg tgccgcacgc
      361 gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa ggcacgtcgg cctccaagtg
      421 gcaatattgg caaattcgaa aatatataca gttgggttgt ttgcgcatat ctatcgtggc
      481 gttgggcatg tacgtccgaa cgttgatttg catgcaagcc gaaattaaat cattgcgatt
      541 agtgcgatta aaacgttgta catcctcgct tttaatcatg ccgtcgatta aatcgcgcaa
      601 tcgagtcaag tgatcaaagt gtggaataat gttttctttg tattcccgag tcaagcgcag
      661 cgcgtatttt aacaaactag ccatcttgta agttagtttc atttaatgca actttatcca
      721 ataatatatt atgtatcgca cgtcaagaat taacaatgcg cccgttgtcg catctcaaca
      781 cgactatgat agagatcaaa taaagcgcga attaaatagc ttgcgacgca acgtgcacga
      841 tctgtgcacg cgttccggca cgagctttga ttgtaataag tttttacgaa gcgatgacat
      901 gacccccgta gtgacaacga tcacgcccaa aagaactgcc gactacaaaa ttaccgagta
      961 tgtcggtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
     1021 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
     1081 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
     1141 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
     1201 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
     1261 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
     1321 atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
     1381 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
     1441 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
     1501 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
     1561 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc
     1621 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     1681 aacaattata aatgtcaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     1741 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     1801 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     1861 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     1921 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     1981 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     2041 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     2101 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta
     2161 cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc
     2221 caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc
     2281 tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata
     2341 tcacaaactg gaaatgtcta tcaatatata gttgctgatc agatccagac atgataagat
     2401 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2461 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttccga
     2521 gtttgtcaga aagcagacca aacagcggtt ggaataatag cgagaacaga gaaatagcgg
     2581 caaaaataat acccgtatca cttttgctga tatggttgat gtcatgtagc caaatcggga
     2641 aaaacgggaa gtaggctccc atgataaaaa agtaaaagaa aaagaataaa ccgaacatcc
     2701 aaaagtttgt gttttttaaa tagtacataa tggatttcct tacgcgaaat acgggcagac
     2761 atggcctgcc cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg
     2821 cgctcagctg gaattcagat ctgcggccgc ttacatgtct cgatcccact taactatctt
     2881 gggctgtgac aaagtcacat ggttcacacg gcaggcatac tcatcttttt cagtgggggt
     2941 gaattcagtg tagtacaaga gatagaaaga ccagtccttg ctgaaagaca agtctgaatg
     3001 ctccactttt tcaattctct ctccattctt cagtaagtca acttcaatgt cggatggatg
     3061 aaacccagac acatagcaat tcaggaaatt tgactttcca ttctctgctg gatgacgtga
     3121 gtaaacctga atctttggag tacgctggat agcctccagg ccagaaagag agagtagcgc
     3181 gagcacagct aaggccacgg agcgagacat agatctgtga ttgtaaataa aatgtaattt
     3241 acagtatagt attttaatta atatacaaat gatttgataa taattcttat ttaactataa
     3301 tatattgtgt tgggttgaat taaaggtccc ggcatcctca aatgcataat ttcatagtcc
     3361 cccttgttgt aagtgatgcg tatttctgaa tctttgtaaa atagcacaca agactccaac
     3421 gcgtttggcg ttttattttc ttgctcgact ctagaggatc gatcccccgg atctgatcat
     3481 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     3541 agttttgtaa taaaaaaacc tataaatatc ggatcctata aatatgagga accctggaca
     3601 ctttgccatc ctttgcctcc tattggtgac catagaccgg ccccagtatt gtgaaggctc
     3661 acactggctg aagactttca ggattgtaat aatggagcct ggcatattgg agccccggtt
     3721 catccaggtc agctatgtag actccataca gtatcaggga ttcgacagca gatcagagac
     3781 tgcggggatg caacctcggg ctgcttggat gaagcaggag ccacctgaat attggaagaa
     3841 tgagacagaa catgccatgg gcgcgtcact gttagcaaga cgaactctta tatacatggt
     3901 gactgaaaat aataacaaga agaatgacta tcacaccctg caggaagtgt ttggctgcaa
     3961 tgtggcacat gatgggagct tccttggtgg tcactacggg ctcacctact atggctatga
     4021 ttacatcatc ctgaatgagg acctgaactc ttggactaca gagggaaaag taggtggaaa
     4081 atttaatcca gaccggacgc aaggaagcgt gacagaaggt tggaggactt acctgaaagg
     4141 ggagtgcaca gaaaggtttc ttagatgcct ggaccttgga aaggagacct tgctgcgctc
     4201 cgatgccccc cgaacacatg tgacccacaa agtaacacct gaagggaatg tcaccctgag
     4261 gtgctgggct ctgggtttct acccagctga catcaccctg acctggaaga gagatgggaa
     4321 aaaccacacc caggacatgg agctgccaga caccaggcct gcaggggatg gaaccttcca
     4381 gaagtgggca gctgtggtgg ttccttttgg agaagagctg agatacacat gtcatgtgca
     4441 ccatgaaggg ttacctgggc cactcactct gaaatggggg atcgaaggtc gccatcatca
     4501 tcatcatcat tagggatccg ggttattagt acatttatta agcgctagat tctgtgcgtt
     4561 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
     4621 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
     4681 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
     4741 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
     4801 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
     4861 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca
     4921 attgactcga cgtaaacacg ttaaataaag cttggacata tttaacatcg ggcgtgttag
     4981 ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag
     5041 acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca
     5101 aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca
     5161 atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg
     5221 gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta
     5281 ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg
     5341 atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta
     5401 ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt
     5461 cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt
     5521 ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag
     5581 gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt
     5641 ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa
     5701 cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg
     5761 aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga
     5821 tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcggat
     5881 cgatcccgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
     5941 acttcgctgt cgtcgacgta catgtatgct ttgttgtcaa aaacgtcgtt ggcaagcttt
     6001 aaaatattta aaagaacatc tctgttcagc accactgtgt tgtcgtaaat gttgtttttg
     6061 ataatttgcg cttccgcagt atcgacacgt tcaaaaaatt gatgcgcatc aattttgttg
     6121 ttcctattat tgaataaata agattgtaca gattcatatc tacgattcgt catggccacc
     6181 acaaatgcta cgctgcaaac gctggtacaa ttttacgaaa actgcaaaaa cgtcaaaact
     6241 cggtataaaa taatcaacgg gcgctttggc aaaatatcta ttttatcgca caagcccact
     6301 agcaaattgt atttgcagaa aacaatttcg gcgcacaatt ttaacgctga cgaaataaaa
     6361 gttcaccagt taatgagcga ccacccaaat tttataaaaa tctattttaa tcacggttcc
     6421 atcaacaacc aagtgatcgt gatggactac attgactgtc ccgatttatt tgaaacacta
     6481 caaattaaag gcgagctttc gtaccaactt gttagcaata ttattagaca gctgtgtgaa
     6541 gcgctcaacg atttgcacaa gcacaatttc atacacaacg acataaaact cgaaaatgtc
     6601 ttatatttcg aagcacttga tcgcgtgtat gtttgcgatt acggattgtg caaacacgaa
     6661 aactcactta gcgtgcacga cggcacgttg gagtatttta gtccggaaaa aattcgacac
     6721 cacaactatg cacgttcgtt tgactggtac gccgtcggcg tgttaacata caagttgcta
     6781 accggcggcc gacacccatt tgaaaaaagc gaagacgaaa tgttggactt gaatagcatg
     6841 aagcgtcgtc agcaatacaa tgacattggc gttttaaaac acgttcgtaa cgttaacgct
     6901 cgtgactttg tgtactgcct aacaagatac aacatagatt gtagactcac aaattacaaa
     6961 caaattataa aacatgagtt tttgtcgtaa aaatgccact tgttttacga gtagaattag
     7021 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7081 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7141 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     7201 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     7261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7441 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     7501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7561 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     7621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7681 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     7741 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7801 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7861 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     7921 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7981 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8041 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8101 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8161 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8221 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8281 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8341 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8401 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8461 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8521 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8581 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8641 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8701 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8761 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8821 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8881 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8941 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9001 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9061 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9121 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9181 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9241 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9301 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9361 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9421 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9481 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9541 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9661 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9721 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9781 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9841 accgctcgcc gc
//
