LOCUS       sequence-189978-        6833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP2.4.1
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             703..711
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1842..1859)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1848..2072
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2204..2792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2284..2303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3121..3168
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3193..3212
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3400..4194)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3400..4194)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3511..3530)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4121..4140
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4229..4586)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4243..4378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4395..4586
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4543..4563
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4646..5437,5438..5506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5269..5288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5507..5611)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5638..6093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5725..5744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5935..5956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6105..6484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6485..6688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6639..6659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6733..6752
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6733..6751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcgagccat gggttgctct ttttctatct tcttacttgc ccttctgtcg tgccttacag
       61 tgccagcgtc tgcggtggaa gttaggaacg tcagttctag ctactatgcc accaatgatt
      121 gctcgaacag cagcatcacc tggcaactca acaacgcagt tctccacctt cctggatgcg
      181 tcccatgtga gaatgataac ggcaccttgc actgctggat acaagtaaca ccaaatgtgg
      241 ctgtgaaaca ccgcggcgcg ctcactcata acctgcggac gcacgtcgat atgatcgtaa
      301 tggcagctac ggtctgttcg gccctgtatg tgggagacat gtgcggggcc gtgatgattg
      361 tgtcgcaggc tctcataata tcgccagaac gccacaactt cacccaagag tgcaactgtt
      421 ccatctacca aggtcatatc actggccatc gcatggcatg ggacatgatg ctaaactggt
      481 caccaactct taccatgatc cttgcttatg ccgctcgtgt tcccgagcta gtccttgaag
      541 ttgtctttgg tggtcattgg ggtgtggtat ttggcttggc ctatttctcc atgcagggag
      601 cgtgggccaa ggtcattgcc atcctccttc ttgttgcggg agtggacgca gagactcata
      661 ccaccggctc agctgcgggt catgccgcaa gggggtttgc caacctcctt agtcctggtt
      721 ccaagcaaaa aataagtttg atcaatacca atggcagctg gcacataaac cggaccgccc
      781 ttaattgtaa tgacagcttg cagacgggtt tcctcgcttc tttgttttac gtcaacaact
      841 tcaacagctc tggctgcccc gagcgcatgt cttcctgccg tgggctggac gatttccgca
      901 tcgggtgggg aaccctggaa tacgagacca acgtcaccaa cgaggaggac atgaggccgt
      961 actgctggca ttaccctccg aagccttgcg gcattatctc ggctaggaca gtttgcgggc
     1021 cggtctactg tttcactcct agccctgttg ttgtgggtac tactgacgag cagggcttgc
     1081 ccacctatag ctggggggaa aatgagaccg atgtcttcct gttaaatagc acaagacccc
     1141 cgcaaggagc ttggttcggc tgcacttgga tgaatgggac tgggtttact aagacatgcg
     1201 gtgcaccacc ttgccgcatt aggagggatt tcaacagcac cctcgatcta ctgtgcccca
     1261 cagactgctt tagaaagcac ccggacacca cctatctcaa gtgtggagca gggccttggt
     1321 taacccccag atgcctggta gactaccctt atagattgtg gcattatccg tgcaccgtaa
     1381 attttaccat ctttaaggtg cggatgtatg tggggggggt ggagcatcga ttgtcagcag
     1441 catgcaactt cacgcgcggg gaccgctgca gattggagga cagagatagg ggtcagcaga
     1501 gcccactgct gcattccacc actgagtggg cggtattacc atgctctttc tccgacttac
     1561 cggcactgtc cactggtcta ttgcacctcc atcaaaatat cgtggacgtg cagtatctct
     1621 atggacttac tccggctatc acaaaataca tcgtgaagtg ggagtgggtg gttctcctct
     1681 ttttgctgtt ggcagacgcc agggtctgtg cgtgcctttg gatgctcatc atattgggcc
     1741 aagccgaagc gtaatctaga aagtttaagc cgaattctgc agatatccag cacagtggcg
     1801 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     1861 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     1921 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     1981 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2041 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagtggcgg
     2101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2641 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2941 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     3001 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     3061 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     3121 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     3181 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     3241 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     3301 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     3361 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3421 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3481 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3541 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3601 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     3661 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     3721 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     3781 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     3841 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     3901 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     3961 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     4021 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4081 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4141 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     4201 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc
     4261 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4321 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4381 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     4441 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     4501 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     4561 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta
     4621 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4681 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4741 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4801 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4861 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4921 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4981 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5041 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5101 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5161 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5221 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5281 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5341 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5401 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5461 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5521 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5581 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6061 cgaattttaa caaaatatta acgcttacaa tttacgcgcg cgttgacatt gattattgac
     6121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     6181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     6241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     6301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     6361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     6421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     6481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     6541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     6601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     6661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
     6721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt
     6781 aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcggc ttg
//
