LOCUS       sequence-189979-        6833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP2.5.1
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1841..1858)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1847..2071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2203..2791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2283..2302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3120..3167
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3192..3211
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3399..4193)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3399..4193)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3510..3529)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4120..4139
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4228..4585)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4242..4377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4394..4585
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4542..4562
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4645..5436,5437..5505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5268..5287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5506..5610)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5637..6092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5724..5743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5934..5955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6104..6483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6484..6687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6638..6658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6732..6751
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6732..6750
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgagccatg ggttgctctt tttctatctt cttacttgct cttctgtcat gcgtcacagt
       61 gccagtgtct gcagtggagg tcaggaacat cagttctagc tactacgcca ctaatgactg
      121 ctcgaacaac agcatcacct ggcagctcac taacgcagtt ctccacctcc ccggatgcgt
      181 cccgtgtgag aataacaatg gcaccttgca ttgctggata caagtaacac ctaatgtggc
      241 tgtaaagcat cgcggtgcac tcactcacag cctgcgagca catgtcgaca tgatcgtaat
      301 ggcagctacg gtctgctcgg ccttgtacgt aggagacgtg tgtggtgctg tgatgattgc
      361 gtctcaggcc ctcatagtat cgccagaata ccacaacttc acccaagagt gcaactgctc
      421 cgtctatcaa ggccatatca ccggccaccg catggcatgg gacatgatgc tgaactggtc
      481 tccaactctt accatgatcc tcgcctacgc tgctcgtgtt cctgagctag tccttgagat
      541 tgtcttcggc ggccattggg gtgtggtgtt tggcttggcc tatttctcca tgcagggagc
      601 gtgggccaaa gtctttgcca tcctcctttt tgtcgcagga gtggatgcac acacccgcct
      661 catcggttca gtggcgggtc gcaacactga cacccttcgt agcttatttg ctactggtgc
      721 ccagcagaag attagcctga tcaacaccaa cggcagctgg cacataaatc ggaccgccct
      781 caattgcaat gattccttgc aaacgggttt ccttgcttcc ctgttttatg tcaaaaactt
      841 caacagctct ggctgccccg agcgtatgtc ttcctgccgt gagctggatg attttcgcat
      901 cgggtgggga accttggaat atgagactaa tgccaccaat gatgaagaca tgaggccgta
      961 ttgttggcat tacccaccaa ggccttgcgg catagtctcg gctaggacgg tttgcgggcc
     1021 ggtttactgc tttaccccta gccctgttgt cgtgggtacc actgaccggc agggcgtgcc
     1081 cacctatagc tggggggaaa atgagaccga tgtcttcctg ctaaatagca caagaccccc
     1141 gcaaggagct tggttcggct gcacctggat gaacgggact gggtttacta agacttgcgg
     1201 cgcgccaccc tgccgcatca ggaaggacca taacagcacc ctcgatctat tgtgccccac
     1261 agactgtttt aggaagcatc cagatactac ctatctcaag tgcggagcag ggccttggtt
     1321 aacccccaga tgcttggtag attaccctta tagactgtgg cattatccgt gcacagtgaa
     1381 ttttaccatc ttcaaggtga ggatgtatgt ggggggggtg gagcatcgat tgaacgcggc
     1441 gtgcaacttc acgcgcggag atcgctgcag attggaggac agggataggg gtcagcagag
     1501 tccgctgcta cattccacca ctgagtgggc ggtgttgcca tgctctttct ctgacctacc
     1561 ggcactatcc actggtctat tgcacctcca ccaaaacatc gtggacgtgc agtacctcta
     1621 tggacttact ccggctatca caagatacat cgtgaagtgg gaatgggtgg tcctcctttt
     1681 cttgttattg gcggacgcca gagtctgtgc atgcctttgg atgctcatca tactgggcca
     1741 agccgaagcg taatctagaa agtttaagcc gaattctgca gatatccagc acagtggcgg
     1801 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     1861 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1921 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     1981 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2041 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagtggcggt
     2101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2941 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3001 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3061 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3121 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3181 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     3241 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3301 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     3361 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     3421 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     3481 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     3541 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     3601 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     3661 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     3721 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     3781 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     3841 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     3901 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     3961 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4021 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4081 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4141 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4201 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     4261 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4321 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     4381 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4441 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     4501 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     4561 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aataaatcaa tctaaagtat
     4621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4681 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5041 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5641 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5701 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5761 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5821 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5881 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5941 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6001 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6061 gaattttaac aaaatattaa cgcttacaat ttacgcgcgc gttgacattg attattgact
     6121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     6181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     6541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     6661 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     6721 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta
     6781 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattcggct tgc
//
