LOCUS       sequence-189982-        6826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP3.1.1
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1837..1854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1843..2067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2199..2787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2279..2298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3116..3163
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3188..3207
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3395..4189)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3395..4189)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3506..3525)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4116..4135
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4224..4581)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4238..4373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4390..4581
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4538..4558
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4641..5432,5433..5501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5264..5283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5502..5606)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5633..6088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5720..5739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5930..5951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6100..6479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6480..6683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6634..6654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6728..6747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6728..6746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagacaagct tatgggttgc tctttttcta tcttccttct tgctctgttc tcttgcttaa
       61 tccatccagc agctagttta gagtggcgga acacgtctgg cctctacatc cttaccaacg
      121 actgccccaa tagcagtatt gtgtatgagg ccgatgacgt tattctgcac acacctggct
      181 gtataccttg tgttcaggac ggcaatacat ccacgtgctg gacctcagtg acacctacag
      241 tggcagtcag atacgtcgga gctaccaccg cttcgatacg cagtcatgta gacctgctgg
      301 taggcgcggc cacgatgtgc tctgcgctct acgtgggtga cgcgtgtggg gccgtcttcc
      361 tcgtgggaca agccttcacg ttcagacccc gtcgccatca aacggtccag acctgcaact
      421 gctcgctgta cccaggccat ctcacgggac atcgaatggc ttgggatatg atgatgaatt
      481 ggtcccccgc tgtgggtatg gtagtggcgc acgtcctgcg tctgccccag accttgttcg
      541 acataatagc cggggcccat tggggcatct tggcgggcct agcctattac tccatgcagg
      601 gcaactgggc caaggtcgtt atcatcatgg ttatgttttc aggggttgac gcttctacat
      661 acaccaccgg tggccataca gctcgtggtg ctcgtgggat agctagcttt tttgatctgg
      721 gcgcccgaca gaacctgcag ctggttaaca ccaatggctc gtggcacatc aacaggacag
      781 ctctgaactg caatgagtcc ataaacacgg ggttcatagc agggttgttt tattatcata
      841 agttcaactc tactggatgt cctcaaaggc tcagcagctg caagcccatc acttccttca
      901 ggcaggggtg gggccctttg acagatgcta acatcaccgg ttcctctgat gacaaaccgt
      961 actgctggca ctacgcacct agaccttgtg aatctgtccc agcatcaaaa gtctgcggcc
     1021 ctgtgtactg ctttacacca tcgccagtgg ttgtaggcac tactgatgct aagggcgtcc
     1081 caacctacac ctggggtgcg aatgagacag acgtgttcct gctgaattcc ctgcggcctc
     1141 ccaaaggtcg gtggttcggg tgcacctgga tgaactccac ggggtttacc aagacgtgcg
     1201 gagccccccc ttgtaacatc tatgggggtg gggggaattc caaaaatgag tcagacctct
     1261 tctgccccac cgactgcttc aggaaacatc ctgaggccac atacagccgg tgtggtgcgg
     1321 ggccttggtt gacacctcgg tgcatggtcg actatccata ccggctttgg cactacccat
     1381 gtacaatcaa tttcacattg ttccaagtga ggatgtttgt gggcgggttt gaacaccggt
     1441 ttaccgccgc ttgcaactgg accagggggg agcgctgcga tatcgaggat cgcgaccgca
     1501 gcgagcaaca tccgctgcta cattcaacaa ctgagctcgc tatactgcct tgctccttca
     1561 cgcccatgcc tgcattgtcg acaggtctaa tacacctcca ccaaaacatc gtggatgtcc
     1621 aataccttta tggcgttgga tctggcatgg tgggatgggc gttgaaatgg gagttcgttg
     1681 tcctcgtctt cctcctccta gcagatgcac gcgtgtgcgt tgccctttgg ttgatgctga
     1741 tgatatcaca agcagaagca taagccgaat tctgcagata tccagcacag tggcggccgc
     1801 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1861 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1921 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1981 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2041 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     2101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2941 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     3001 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     3061 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     3121 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     3181 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3241 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3301 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3361 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3421 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3481 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3541 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3601 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3661 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     3721 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     3781 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     3841 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     3901 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     3961 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     4021 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     4081 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     4141 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     4201 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     4261 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     4321 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     4381 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     4441 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     4501 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     4561 agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat
     4621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     5641 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5701 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5761 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5821 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5881 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5941 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6001 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6061 tttaacaaaa tattaacgct tacaatttac gcgcgcgttg acattgatta ttgactagtt
     6121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     6361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     6541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     6661 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     6721 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     6781 tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcggct
//
