LOCUS       sequence-190011-        6824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP4.1.1
FEATURES             Location/Qualifiers
     source          1..6824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     635..654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        635..653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      741..750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2568..2585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2574..2798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2930..3518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3010..3029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3847..3894
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3919..3938
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4126..4920)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4126..4920)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4237..4256)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4847..4866
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4955..5312)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4969..5104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5121..5312
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5269..5289
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(5372..6163,6164..6232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5995..6014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6233..6337)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6364..6819
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6451..6470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6661..6682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      601 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      661 aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg
      721 gtggaattcg gcttacaggg gccgccatgg gttgctcttt ttctatcttc ctcttggcac
      781 ttctctcgtg cctgactgtc cccgcttcgg ccattaacta tcgcaatgtc tcgggcatct
      841 accatgtcac caacgattgc ccgaattcaa gcatagtgta tgagaccgaa catcacatct
      901 tgcacattcc aggttgcgtg ccctgtgtga gagtggggaa tcagtcaagc tgctgggtgg
      961 cccttactcc taccgtcgct acgccataca tcggcgcgcc gcttgagtcc ttgcggagtc
     1021 atgtggatct gatggtggga gctgccactg cctgctcagc cctttacatc ggggatttgt
     1081 gtggcggtgt gtttttggtt ggtcagatgt tctccttccg accgcggcgc cactggacta
     1141 cccaggattg caattgttcc atctatacag ggcacatcac tggccacaga atggcctggg
     1201 acatgatgat gaactggagt ccaacagcca ccttagttct cgcccagctt atgaggatcc
     1261 caggtgcctt agtagaccta cttgctggag gtcactgggg tgtcctcgtg ggagtggcct
     1321 atttctgcat gcaagccaat tgggccaaag tcatcttagt cctattcctc tttgcagggg
     1381 ttgatgctca gacacgtttg actgggggca cacctgccca cggcgcacag agcatagcta
     1441 aattgtttag cgctggacct cggcaaaatg tacagctcat taacagcaac gggagctggc
     1501 atataaacag gactgccctc aactgcaatg acagcttaaa cactgggttc ttgactggct
     1561 tgttctacgt ctccaggttc aacagctcag ggtgtcccga gcggctcgca agttgcaaga
     1621 gccttgacag ctttaaccag ggctggggcc cactaggggc cgctaacatc agcggcccgt
     1681 ctgacgacaa gccttattgc tggcactacg cgcctcgacc gtgcgggatt gtgccggcat
     1741 ccagtgtgtg tggccccgtg tattgtttca ctcccagccc tgtcgtggtc ggaaccacag
     1801 accgtttcgg ggtcccgact tacacctggg gggataatga gtctgatgtc ttccttttga
     1861 acgcgaccag accgccacgt ggtgcgtggt tcgggtgcgt atggatgaac agtaccgggt
     1921 ttacaaaaac ctgtggtgct cccccatgtg aggtcaagac caacaatgga acctggagct
     1981 gccccaccga ttgcttcagg aagcacccag cgaccacgta caccaagtgc gggtcggggc
     2041 cctggatcac accacgatgc ttggtccact atccgtaccg gctgtggcat ttcccatgta
     2101 ccctcaattt taccatcttc aaggtcagga catacatcgg cggcatagag caccggatgg
     2161 aagtggcatg caactggact aggggggaag tctgtggctt agagcatagg gatcgtgcag
     2221 aactgtcacc tctgctcctt tccactacga cgtggcaagt cctcccctgc tcttttacca
     2281 ctctacctgc cctttccacc ggcttgatcc acctccacca aaatatcgtg gacgtccaat
     2341 acctctacgg tgttggatct gcggtagtat cttgggctct caagtgggag tatgtggtgc
     2401 ttgtgtttct gcttcttgca gacgcgagga tctctgcttg cctgtggatg atgttcatgg
     2461 taagtcaagt tgaggcgtaa tctagaaacc ttaagccgaa ttctgcagat atccagcaca
     2521 gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     2581 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2641 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2701 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2761 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2821 tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3661 gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc
     3721 ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt
     3781 ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc
     3841 gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag
     3901 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg
     3961 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     4021 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     4081 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     4141 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     4201 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     4261 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     4321 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     4381 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     4441 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     4501 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     4561 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     4621 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     4681 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     4741 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     4801 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     4861 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     4921 gcgaaacgat cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa
     4981 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     5041 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     5101 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     5161 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     5221 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     5281 acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat aaatcaatct
     5341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6361 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6781 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcg
//
