LOCUS       sequence-190013-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP4.2.1
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1826..1843)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1832..2056
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2188..2776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2268..2287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3105..3152
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3177..3196
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3384..4178)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3384..4178)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3495..3514)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4105..4124
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4213..4570)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4227..4362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4379..4570
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4527..4547
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4630..5421,5422..5490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5253..5272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5491..5595)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5622..6077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5709..5728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5919..5940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6089..6468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6469..6672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6623..6643
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6717..6736
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6717..6735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      join(6823..6827,1..5)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catgggttgc tctttttcta tcttcctttt ggcacttctc tcgtgcctga ctgtccccgc
       61 gtcggccact aactatcgca atgtctcggg catctaccac gtcaccaatg actgcccgaa
      121 ctcaagtata gtgtatgagg ccgaccatca catcttgcac ctcccaggct gcgtgccctg
      181 cgtgaggacg gggaatcagt cacactgctg ggtggctctt actcctactg tcgcagcgcc
      241 atacatcggc gcaccgcttg agtctcttcg gagtcatgtg gatttgatgg tgggaactgc
      301 cactgcttgc tcggcccttt acatcgggga tctgtgtggt ggcttgttcc tggttggtca
      361 gatgttctct ttccgaccgc gacgccactg gactacccag gagtgcaatt gttccatcta
      421 tacagggcac ctcactggcc acagaatggc ttgggacatg atgatgaact ggagtccaac
      481 aaccacttta gttcttgccc aggtcatgag gatcccaggt actctggtag acctgctcgc
      541 tggaggtcac tggggcgtcc tcgtgggagt agcctatttc agcatgcaag ccaattgggc
      601 caaggtcatc ttagtcctat tcctctttgc aggagttgac gccagcactc acatatctgg
      661 agggacagct ggccgagcta ccgccggctt cactgagctc tttacaccag gagctaagca
      721 aaatgtgcag ctcattaaca gcaatgggag ttggcatata aacaggactg ccctcaactg
      781 caatgatagt ttaaacactg gattcctggc tggcctgttc taccactaca gctttaatag
      841 ctcagggtgc cccgaacggc tcgcgagctg caagagcctt gacgaccatc accaaggctg
      901 gggcccactc gggatcgcta acatcagcgg cccgtctaac gacaggcctt attgctggca
      961 ctacgcgcct cggccgtgcg ggattgtgcc ggcatccagt gtgtgtggcc ccgtctactg
     1021 tttcactccc agccccgtcg tggtcggaac cactgaccgc ctcggggtcc ccacttacaa
     1081 ctggggggag aatgagactg atgtcttctt actcaactca accagaccgc cgcggggggc
     1141 atggtttgga tgcgtgtgga tgaatggcac cgggtttaca aagacttgtg gcgcccctcc
     1201 atgcgtggtc aacgccaata ccaataacac ttggcactgc cccaccgatt gcttcaggaa
     1261 gcatccggag accacttacg ccaagtgcgg gtcagggccg tggatcacac cacggtgctt
     1321 gattgactac ccgtaccggc tgtggcattt cccatgtacc atcaatttta ccatctttaa
     1381 gatcaggaca tttgtcggcg ggatagagca tcggatggca gcggcatgca actggaccag
     1441 gggggaagtc tgtggcttgg agcataggga tcgtgcagag ttatcacctc tgctcctttc
     1501 caccaccacg tggcaaatcc tcccctgctc tttcaccact ctacctgccc tttccaccgg
     1561 cttgatccac ctccaccaaa atatcgtgga cgtccagtac ctctacggtg ttggatctgc
     1621 ggtggtatct tgggccctca aatgggaata tgtggtgctc gtgttcctgc ttctcgcgga
     1681 cgcgagagtc tccgcttgcc tatggatgat gtttatggta agtcaagttg aggcgtaatc
     1741 tagaaacctt aagccgaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
     1801 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1861 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1921 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1981 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2041 gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac ggttatccac
     2101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc
     2941 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     3001 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     3061 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     3121 accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
     3181 ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc
     3241 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     3301 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     3361 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3421 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3481 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3541 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3601 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     3661 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3721 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3781 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3841 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     3901 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3961 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     4021 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4081 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4141 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     4201 tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     4261 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     4321 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     4381 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     4441 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     4501 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt
     4561 tccgcctcag gactcttcct ttttcaataa atcaatctaa agtatatatg agtaaacttg
     4621 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4681 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4741 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4801 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4861 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4921 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4981 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5041 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5101 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5161 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5221 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5281 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5341 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5401 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5461 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5521 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5581 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     5641 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5701 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5761 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5821 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5881 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5941 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6001 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6061 attaacgctt acaatttacg cgcgcgttga cattgattat tgactagtta ttaatagtaa
     6121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     6181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     6241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     6301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     6361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     6421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     6481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     6541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     6601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     6661 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     6721 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc
     6781 tcggatccac tagtccagtg tggtggaatt cggcttacag gggccgc
//
