LOCUS       sequence-190021-        2881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 11648 to 12279) in pENTR3C, Gateway Entry 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  TITLE     Shiang Lab CMYA5 project plasmids (will be published)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMYA5tnC/pENTR3C
FEATURES             Location/Qualifiers
     source          1..2881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      113..199
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      291..318
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     326..349
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    368..467
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1120..1219)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1331..1350)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1342..2151
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1409..1428)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2241..2829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2730..2749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca
       61 tggatctcgg ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa
      121 acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      181 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg
      241 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat
      301 cctgacggat ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct
      361 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      421 taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctctt tcatgggact
      481 ccagctgaaa gttaacctcc aacccaatga taactacttt ttctatgtga gggccatcaa
      541 tgcatttggg acaagtgaac agagtgaagc tgctctcatc tccaccagag gaaccagatt
      601 tctcttgttg agagaaacag ctcatcctgc tctacacatt tcctcaagtg ggacagtgat
      661 cagctttggt gagaggagac ggctgacgga aatcccgtca gtgctgggtg aggagctgcc
      721 ttcctgtggc cagcattact gggaaaccac agtcacagac tgcccagcat atcgactcgg
      781 catctgctcc agctcggctg tgcaggcagg tgccctagga caaggggaga cctcatggta
      841 catgcactgc tctgagccac agagatacac atttttctac agtggtattg tgagtgatgt
      901 tcatgtgact gagcgtccag ccagagtggg catcctgctg gactacaaca accagagact
      961 tatcttcatc aacgcagaga gcgagcagtt gctcttcatc atcaggcaca ggtttaatga
     1021 gggtgtccac cctgcctttg ccctggagaa acctggaaaa tgtactttgc acctggggat
     1081 agagcccccg gattctgtaa ggcacaagga attatctaga cccagctttc ttgtacaaag
     1141 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1201 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     1261 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1321 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1381 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1441 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1501 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1561 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1621 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1681 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1741 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1801 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1861 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1921 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1981 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2041 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2101 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2161 gttaattggt tgtaacatta ttcagattgg gccccgttcc actgagcgtc agaccccgta
     2221 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2281 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2341 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2401 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2461 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2521 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2581 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2641 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2701 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2761 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2821 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2881 g
//
