LOCUS       sequence-190028-        5250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV genome that ubiquitously expresses the fluorescent protein 
            mNeonGreen from the hSyn1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
  PUBMED    28671695
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593"; 
            journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue: 
            "8"; pages: "1172-1179"
COMMENT     Sequence Label: pAAV-hSyn1-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..5250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   423..552
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(614..1202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(694..713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1373..2164,2165..2233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1996..2015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2234..2338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2406..2424
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2462..2484)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2584..2603
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2620..3075)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2757..2778)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2969..2988
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3150..3290
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3161..3290
                     /label=AAV2 ITR
     polyA_signal    3330..3806
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     3588..3607
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    3838..4426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3944..3955
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4353..4373
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4451..5161)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4897..4906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5158..5167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(5181..5250,1..378))
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg gtgcggtccg
       61 gctgggccgg cggcggcgcg gacgcgacca aggtggccgg gaaggggagt ttgcggggga
      121 ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc ggtggcgcgc gccgccaggc
      181 gggggcgaag gcactgtccg cggtgctgaa gctggcagtg cgcacgcgcc tcgccgcatc
      241 ctgtttcccc tccccctctc tgatagggga tgcgcaattt ggggaatggg ggttgggtgc
      301 ttgtccagtg ggtcggggtc ggtcgtcagg taggcacccc caccccgcct catcctggtc
      361 ctaaaaccca cttgcactca tacgcagggc cctctgcagt ctagacacac gcgtgcggcc
      421 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
      481 ggccgggcga ccaaaggtcg cccgacgccc gggcggcctc agtgagcgag cgagcgcgca
      541 gctgcctgca ggacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      841 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1321 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1381 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1441 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1501 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1561 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1621 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1681 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1741 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1801 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1861 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1921 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1981 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2041 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2101 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2161 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2221 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2281 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2341 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2401 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2461 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2521 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2581 atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt
     2641 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa
     2701 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag
     2761 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2821 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
     2881 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2941 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg
     3001 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3061 cgctacaggg cgcgtactat ggttgctttg acgtatgcgg tgtgaaatac cgcacagatg
     3121 cgtaaggaga aaataccgca tcaggcgccc ctgcaggcag ctgcgcgctc gctcgctcac
     3181 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
     3241 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgctc
     3301 ggtccgcacg tggttaccta caaaatcaga aggacaggga agggagcagt ggttcacgcc
     3361 tgtaatccca gcaatttggg aggccaaggt gggtagatca cctgagatta ggagttggag
     3421 accagcctgg ccaatatggt gaaaccccgt ctctaccaaa aaaacaaaaa ttagctgagc
     3481 ctggtcatgc atgcctggaa tcccaacaac tcgggaggct gaggcaggag aatcgcttga
     3541 acccaggagg cggagattgc agtgagccaa gattgtgcca ctgcactcca gcttggttcc
     3601 caatagaccc cgcaggccct acaggttgtc ttcccaactt gccccttgct ccataccacc
     3661 cccctccacc ccataatatt atagaaggac acctagtcag acaaaatgat gcaacttaat
     3721 tttattagga caaggctggt gggcactgga gtggcaactt ccagggccag gagaggcact
     3781 ggggaggggt cacagggatg ccacccgtag atctctcgag cagcgctcgg tatcgatgcg
     3841 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     3901 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     3961 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac ataggcgagc agccaaggaa
     4021 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     4081 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     4141 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     4201 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     4261 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     4321 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     4381 gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg ataagcttga
     4441 tatcgaattc tcacttgtac agctcgtcca tgcccatcac atcggtaaag gccttttgcc
     4501 actccttgaa gttgagctcg gtcttggagt gcttgagctc cgtcttacgg aacacgtaca
     4561 tcggctggtt cttcagatag ttagccgcca ttggcttggc aaaggtgtag gtggtccgcg
     4621 cagtgctccg gtagcgcttg ccatttccag tggtgtaact ccacttaaag gtactgatga
     4681 tggttttgtc gttggggtaa gtcttcttcg acctgcacca gtccgcagcg gtcagcgagt
     4741 tggtcatcac aggaccgtca gcagggaaac cagtcccctt cacctgggcc tctcctttga
     4801 tgtggcttcc ctcgtaggtg tagcggtagt taacagtaag ggaggcacca tcttcaaact
     4861 gcattgtgcg atggacttgg tatccggagc catctaccat ggcggcctgg aaaggcgaca
     4921 tcccgtcagg gtagggcagg tactgatgga agccataccc gatatgaggg accagaatcc
     4981 agggggagaa ctggaggtca cccttggtgg acttcaggtt taactcctca taaccatcat
     5041 ttggattgcc ggtgccctga cccaccatgt caaagtccac accgttgatg gagccaaaga
     5101 tgtgtaactc atgtgtcgct gggagagagg ccatgttatc ctcctcgccc ttgctcacca
     5161 tggtggcggt accttctcga ctgcgctctc aggcacgaca cgactcctcc gctgcccacc
     5221 gcagactgag gcagcgctga gtcgccggcg
//
