LOCUS       sequence-190042-       10441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd 
            generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22386"; journalName: "Nature"; date: "2017-06-8- 8"; 
            volume: "546"; issue: "7657"; pages: "243-247"
COMMENT     Sequence Label: pHR-tdMCP-YFP
FEATURES             Location/Qualifiers
     source          1..10441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      442..451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1204..1206,1207..1920)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1246..1267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1507..1526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1854..1875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1986..2574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2039..2059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2457..2468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2577..2593)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2578..2594)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2684..2917
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2942..2960)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2943..2960)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3250..3269)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3483..3502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3602..3624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3662..3680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3748..3852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3853..3921,3922..4713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4071..4090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4884..5472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5373..5392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5626..5643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5713..5733)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5718..6047
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5898..6033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7181..7246
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7376..7396
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7821..7955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7858..7877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7912..7931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8123..8756
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8803..8928
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9425..9658
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9843..9887
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10172..10289
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tgccaccatg gccagcaact tcacccagtt cgtgctggtg
      481 gacaacggcg gcaccggcga cgtgaccgtg gcccccagca acttcgccaa cggcatcgcc
      541 gagtggatca gcagcaacag ccgcagccag gcctacaagg tgacctgcag cgtgcgccag
      601 agcagcgccc agaaccgcaa gtacaccatc aaggtggagg tgcccaaggg cgcctggcgc
      661 agctacctga acatggagct gaccatcccc atcttcgcca ccaacagcga ctgcgagctg
      721 atcgtgaagg ccatgcaggg cctgctgaag gacggcaacc ccatccccag cgccatcgcc
      781 gccaacagcg gcatctacgg cggcgctgga ggtgcaggtg gatccatggc cagcaacttc
      841 acccagttcg tgctggtgga caacggcggc accggcgacg tgaccgtggc ccccagcaac
      901 ttcgccaacg gcatcgccga gtggatcagc agcaacagcc gcagccaggc ctacaaggtg
      961 acctgcagcg tgcgccagag cagcgcccag aaccgcaagt acaccatcaa ggtggaggtg
     1021 cccaagggcg cctggcgcag ctacctgaac atggagctga ccatccccat cttcgccacc
     1081 aacagcgact gcgagctgat cgtgaaggcc atgcagggcc tgctgaagga cggcaacccc
     1141 atccccagcg ccatcgccgc caacagcggc atctacggcg gcgctggagg tgcaggtgga
     1201 tccgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1261 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1321 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1381 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     1441 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1501 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1561 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1621 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1681 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1741 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1801 tacctgagct accagtccaa actgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1861 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     1921 tgaattgcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta
     1981 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2041 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2101 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2161 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2221 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2281 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2341 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2401 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     2461 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     2521 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     2581 accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag
     2641 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2701 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     2761 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     2821 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     2881 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     2941 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     3001 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     3061 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     3121 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     3181 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     3241 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     3301 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     3361 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     3421 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     3481 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3541 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3601 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3661 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3841 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3901 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3961 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4021 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4081 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4141 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4201 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4261 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4321 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4381 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4441 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4501 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4561 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4621 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4681 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5701 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5761 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5821 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5881 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5941 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6001 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     6061 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     6121 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     6181 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     6241 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     6301 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     6361 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     6421 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     6481 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     6541 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     6601 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     6661 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     6721 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     6781 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     6841 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     6901 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     6961 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     7021 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     7081 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     7141 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     7201 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     7261 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     7321 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     7381 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     7441 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     7501 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     7561 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     7621 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     7681 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     7741 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7801 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     7861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7921 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     7981 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     8041 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     8101 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     8161 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     8221 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     8281 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     8341 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     8401 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     8461 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     8521 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     8581 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8641 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8701 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     8761 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8821 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8881 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8941 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9001 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9061 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     9121 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     9181 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9241 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     9301 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     9361 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9421 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9481 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9541 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9601 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9661 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     9721 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     9781 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     9841 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     9901 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     9961 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
    10021 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
    10081 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
    10141 ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10201 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10261 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    10321 tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa agattttatt
    10381 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc
    10441 a
//
