LOCUS       sequence-190053-        9694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BMP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: BMP4_pLX307
FEATURES             Location/Qualifiers
     source          1..9694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2545..2569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2577..2618
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2729..3317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2782..2802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3200..3211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3389..3622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3700..3821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3737..3756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3791..3810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3861..3996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4016..4035)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4017..4035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4045..4062)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4045..4061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4054..4076)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4203..4658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4290..4309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4500..4521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4684..4788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4789..4857,4858..5649)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5007..5026)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5820..6408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6309..6328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6562..6579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6696..6717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6732..6737,6738..6755,6756..6762)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6770..6786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6775..6797
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6794..6810
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6794..6810
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6829..6849
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6831..6849
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6877..7103
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7104..7284
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7331..7456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7949..8182
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8367..8411
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8642..8852
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8664..8860
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8711..8846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8892..9494
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8892..8911)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9388..9408
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9537..9654
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgattcctgg taaccgaatg ctgatggtcg ttttattatg ccaagtcctg ctaggaggcg
     1381 cgagccatgc tagtttgata cctgagacgg ggaagaaaaa agtcgccgag attcagggcc
     1441 acgcgggagg acgccgctca gggcagagcc atgagctcct gcgggacttc gaggcgacac
     1501 ttctgcagat gtttgggctg cgccgccgcc cgcagcctag caagagtgcc gtcattccgg
     1561 actacatgcg ggatctttac cggcttcagt ctggggagga ggaggaagag cagatccaca
     1621 gcactggtct tgagtatcct gagcgcccgg ccagccgggc caacaccgtg aggagcttcc
     1681 accacgaaga acatctggag aacatcccag ggaccagtga aaactctgct tttcgtttcc
     1741 tctttaacct cagcagcatc cctgagaacg aggcgatctc ctctgcagag cttcggctct
     1801 tccgggagca ggtggaccag ggccctgatt gggaaagggg cttccaccgt ataaacattt
     1861 atgaggttat gaagccccca gcagaagtgg tgcctgggca cctcatcaca cgactactgg
     1921 acacgagact ggtccaccac aatgtgacac ggtgggaaac ttttgatgtg agccctgcgg
     1981 tccttcgctg gacccgggag aagcagccaa actatgggct agccattgag gtgactcacc
     2041 tccatcagac tcggacccac cagggccagc atgtcaggat tagccgatcg ttacctcaag
     2101 ggagtgggaa ttgggcccag ctccggcccc tcctggtcac ctttggccat gatggccggg
     2161 gccatgcctt gacccgacgc cggagggcca agcgtagccc taagcatcac tcacagcggg
     2221 ccaggaagaa gaataagaac tgccggcgcc actcgctcta tgtggacttc agcgatgtgg
     2281 gctggaatga ctggattgtg gccccaccag gctaccaggc cttctactgc catggggact
     2341 gcccctttcc actggctgac cacctcaact caaccaacca tgccattgtg cagaccctgg
     2401 tcaattctgt caattccagt atccccaaag cctgttgtgt gcccactgaa ctgagtgcca
     2461 tctccatgct gtacctggat gagtatgata aggtggtact gaaaaattat caggagatgg
     2521 tagtagaggg atgtgggtgc cgctgcccaa ctttcttgta caaagtggtt gatatcggta
     2581 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac
     2641 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat ccgatcgtac
     2701 tgatttaata aatacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     2761 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2821 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2881 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2941 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3001 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3061 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3121 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3181 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3241 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3301 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     3361 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     3421 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3601 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     3661 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     3721 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3781 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3961 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     4021 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4081 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4141 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4201 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4381 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4441 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4501 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4561 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4621 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6781 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6841 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6901 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6961 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     7021 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     7081 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     7141 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7201 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7261 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7321 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7381 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7441 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7501 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7561 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7621 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7681 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7741 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7801 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7861 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7921 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7981 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8041 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8101 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8161 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8221 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8281 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8341 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8401 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8461 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8521 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8581 gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8641 agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct
     8701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8761 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac
     8881 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     8941 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     9001 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     9061 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
     9121 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     9181 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     9241 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     9301 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     9361 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     9421 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     9481 ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     9541 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     9601 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     9661 tattacaggg acagcagaga tccactttgg cgcc
//
