LOCUS       sequence-190058-       10504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FERMT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: FERMT1_pLX307
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3355..3379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3387..3428
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3539..4127
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3592..3612)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4010..4021)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4199..4432
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4510..4631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4547..4566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4601..4620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4671..4806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4826..4845)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4827..4845)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4855..4872)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4855..4871)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4864..4886)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5013..5468
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5100..5119)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5310..5331
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5494..5598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5599..5667,5668..6459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5817..5836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6630..7218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7119..7138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7372..7389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7506..7527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7542..7547,7548..7565,7566..7572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7585..7607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7604..7620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7604..7620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7639..7659
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7641..7659
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7687..7913
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7914..8094
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8141..8266
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8759..8992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9177..9221
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9452..9662
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9474..9670
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9521..9656
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9702..10304
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9702..9721)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10198..10218
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10347..10464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgctgtcatc cactgacttt acatttgctt cctgggagct tgtggtccgc gttgaccatc
     1381 ccaatgaaga gcagcagaaa gacgtcacac tgagagtatc tggagacctt cacgttggag
     1441 gagtgatgct caagttagta gaacagatca atatatccca agactggtca gactttgctc
     1501 tttggtggga acagaagcat tgctggcttc tgaaaaccca ctggaccctg gacaaatatg
     1561 gggtccaggc agatgcaaag cttctcttca cccctcagca taaaatgctg cgccttcgtc
     1621 tgccgaattt gaagatggtg aggttgcgag tcagcttctc agctgtggtt tttaaagctg
     1681 tcagtgatat ctgcaaaatc ctgaatatta gaagatcaga agagctttcc ttgttaaagc
     1741 cgtctggtga ctattttaag aagaagaaga aaaaagacaa aaataataag gaacccataa
     1801 ttgaagatat tctaaacctg gagagttctc caacagcttc aggttcatca gtaagtcctg
     1861 gtttatacag taaaaccatg acccctatat atgaccccat caatggaaca ccagcatcat
     1921 ccaccatgac ttggttcagt gacagccctt tgacggaaca aaactgcagc atcctcgcat
     1981 tcagccaacc cccccagtcc ccagaagcac ttgcggatat gtaccagcct cggtctctgg
     2041 ttgataaagc caagctcaat gcaggttggc tagactcctc acgctccctt atggaacaag
     2101 gcatccaaga ggatgagcag ctgctcttac gatttaaata ttattctttc ttcgacttga
     2161 atcctaaata tgatgctgtc cgaataaacc aactctatga gcaagccagg tgggccattc
     2221 tcttagaaga aattgattgc acagaggaag aaatgttgat ctttgcagct ctacagtacc
     2281 acattagcaa actgtcgttg tctgctgaaa cacaggattt tgcaggcgag tccgaggttg
     2341 atgaaataga agcggcgctt tctaatttgg aagtaaccct agaaggtgga aaagcggaca
     2401 gccttttgga ggacattact gatatcccta aacttgcaga taatctcaaa ttatttaggc
     2461 ccaagaagtt actaccaaaa gctttcaaac aatattggtt tatctttaaa gacacatcca
     2521 tagcatactt taaaaataag gaacttgaac aaggagaacc actagaaaaa ctaaatctta
     2581 gaggctgcga agttgtgccc gatgtaaatg tagcaggaag aaaatttgga atcaagttac
     2641 taatccctgt tgccgatggt atgaatgaaa tgtatttgag atgtgaccat gagaatcaat
     2701 acgcccaatg gatggctgcc tgcatgttgg catcgaaggg caaaaccatg gcagacagct
     2761 cctaccagcc agaggtcctc aacatccttt catttctgag gatgaaaaac aggaactctg
     2821 catctcaggt ggcttccagt ctcgaaaaca tggatatgaa cccagaatgt tttgtgtcac
     2881 cacggtgtgc aaaaagacac aaatccaaac agctggccgc ccggatcctg gaggcgcacc
     2941 agaacgtggc ccagatgccc ctggtcgaag ccaagctgcg gttcatccag gcgtggcagt
     3001 cactgcctga gtttggcctc acctactacc ttgtcagatt taaaggaagc aaaaaagatg
     3061 acattctggg agtttcatat aacaggttga ttaaaattga tgcagccacc gggattccag
     3121 tgacaacatg gagattcaca aatatcaaac agtggaatgt aaactgggaa acccggcagg
     3181 tggtcatcga gtttgaccaa aacgtcttta ctgctttcac ctgcctgagt gcagattgca
     3241 agattgtgca cgagtacatt ggcggctaca ttttcttgtc cacccgctcc aaggaccaga
     3301 atgaaacact cgatgaggac ttgttccaca aattgaccgg cggtcaggat agacccccaa
     3361 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     3421 attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata
     3481 tatcttgtgg aaaggacgat actaaaaccc ttcctcggaa ccgacgcgtt aagtcgacaa
     3541 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3601 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3661 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3721 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3781 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3841 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3901 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3961 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4021 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4081 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     4141 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4201 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4441 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4501 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4621 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4681 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4741 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4801 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4861 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4921 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4981 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5041 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5101 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5161 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5221 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5281 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5341 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5401 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5461 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5821 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6121 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7501 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7561 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7621 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7681 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7741 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7801 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7861 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8101 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8161 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8221 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8281 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8341 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8401 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8461 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8521 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8581 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8641 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8701 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8761 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8821 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8881 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8941 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9001 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9061 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9121 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9181 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9241 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9301 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9361 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
     9421 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
     9481 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9541 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9601 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9661 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
     9721 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9781 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     9841 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     9901 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     9961 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    10021 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    10081 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    10141 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    10201 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    10261 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
    10321 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    10381 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10441 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    10501 cgcc
//
