LOCUS       sequence-190066-        7832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PTEN 3'UTR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Kerwin J, Khan I, Iorns E, Tsui R, Denis A, Perfito N, Errington TM
  TITLE     Replication Study: A coding-independent function of gene and 
            pseudogene mRNAs regulates tumour biology.
  JOURNAL   Elife. 2020 Apr 21;9. pii: 51019. doi: 10.7554/eLife.51019.
  PUBMED    32314732
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-21"; volume: "9. pii: 51019. doi"; pages: " 
            10.7554/eLife.51019"
COMMENT     Sequence Label: pCMV-PTEN-3'UTR
FEATURES             Location/Qualifiers
     source          1..7832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        99..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     603..627
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          755..1133
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     polyA_signal    4616..5092
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4815..4834)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5141..5158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5312..5900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5392..5411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6071..6862,6863..6931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6694..6713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6932..7036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7104..7122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7160..7182)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7282..7301
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7318..7773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7455..7476)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7667..7686
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accgcatcag gcgctggcct aaagcggccg cacgcgtcta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      661 catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc gggaacggtg
      721 cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag
      781 gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact
      841 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca
      901 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa
      961 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac
     1021 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc
     1081 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg
     1141 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga ttcgaacatc
     1201 gattgaattc cccggggatc ctagaggagc cgtcaaatcc agaggctagc agttcaactt
     1261 ctgtaacacc agatgttagt gacaatgaac ctgatcatta tagatattct gacaccactg
     1321 actctgatcc agagaatgaa ccttttgatg aagatcagca tacacaaatt acaaaagtct
     1381 gaattttttt ttatcaagag ggataaaaca ccatgaaaat aaacttgaat aaactgaaaa
     1441 tggacctttt ttttttttta atggcaatag gacattgtgt cagattacca gttataggaa
     1501 caattctctt ttcctgacca atcttgtttt accctataca tccacagggt tttgacactt
     1561 gttgtccagt tgaaaaaagg ttgtgtagct gtgtcatgta tatacctttt tgtgtcaaaa
     1621 ggacatttaa aattcaatta ggattaataa agatggcact ttcccgtttt attccagttt
     1681 tataaaaagt ggagacagac tgatgtgtat acgtaggaat tttttccttt tgtgttctgt
     1741 caccaactga agtggctaaa gagctttgtg atatactggt tcacatccta cccctttgca
     1801 cttgtggcaa cagataagtt tgcagttggc taagagaggt ttccgaaggg ttttgctaca
     1861 ttctaatgca tgtattcggg ttaggggaat ggagggaatg ctcagaaagg aaataatttt
     1921 atgctggact ctggaccata taccatctcc agctatttac acacaccttt ctttagcatg
     1981 ctacagttat taatctggac attcgaggaa ttggccgctg tcactgcttg ttgtttgcgc
     2041 attttttttt aaagcatatt ggtgctagaa aaggcagcta aaggaagtga atctgtattg
     2101 gggtacagga atgaaccttc tgcaacatct taagatccac aaatgaaggg atataaaaat
     2161 aatgtcatag gtaagaaaca cagcaacaat gacttaacca tataaatgtg gaggctatca
     2221 acaaagaatg ggcttgaaac attataaaaa ttgacaatga tttattaaat atgttttctc
     2281 aattgtaacg acttctccat ctcctgtgta atcaaggcca gtgctaaaat tcagatgctg
     2341 ttagtaccta catcagtcaa caacttacac ttattttact agttttcaat cataatacct
     2401 gctgtggatg cttcatgtgc tgcctgcaag cttctttttt ctcattaaat ataaaatatt
     2461 ttgtaatgct gcacagaaat tttcaatttg agattctaca gtaagcgttt tttttctttg
     2521 aagatttatg atgcacttat tcaatagctg tcagccgttc cacccttttg accttacaca
     2581 ttctattaca atgaattttg cagttttgca cattttttaa atgtcattaa ctgttaggga
     2641 attttacttg aatactgaat acatataatg tttatattaa aaaggacatt tgtgttaaaa
     2701 aggaaattag agttgcagta aactttcaat gctgcacaca aaaaaaagac atttgatttt
     2761 tcagtagaaa ttgtcctaca tgtgctttat tgatttgcta ttgaaagaat agggtttttt
     2821 tttttttttt tttttttttt aaatgtgcag tgttgaatca tttcttcata gtgctccccc
     2881 gagttgggac tagggcttca atttcacttc ttaaaaaaaa tcatcatata tttgatatgc
     2941 ccagactgca tacgatttta agcggagtac aactactatt gtaaagctaa tgtgaagata
     3001 ttattaaaaa ggtttttttt tccagaaatt tggtgtcttc aaattatacc ttcaccttga
     3061 catttgaata tccagccatt ttgtttctta atggtataaa attccatttt caataactta
     3121 ttggtgctga aattgttcac tagctgtggt ctgacctagt taatttacaa atacagattg
     3181 aataggacct actagagcag catttataga gtttgatggc aaatagatta ggcagaactt
     3241 catctaaaat attcttagta aataatgttg acacgttttc cataccttgt cagtttcatt
     3301 caacaatttt taaattttta acaaagctct taggatttac acatttatat ttaaacattg
     3361 atatatagag tattgattga ttgctcataa gttaaattgg taaagttaga gacaactatt
     3421 ctaacacctc accattgaaa tttatatgcc accttgtctt tcataaaagc tgaaaattgt
     3481 tacctaaaat gaaaatcaac ttcatgtttt gaagatagtt ataaatattg ttctttgtta
     3541 caatttcggg caccgcatat taaaatgtaa ctttattgtt ccaatatgta acatggaggg
     3601 ccaggtcata aataatgaca ttataatggg cttttgcact gttattattt ttcctttgga
     3661 atgtgaaggt ctgaatgagg gttttgattt tgaatgtttc aatgtttttg agaagccttg
     3721 cttacatttt atggtgtagt cattggaaat ggaaaaatgg cattatatat attatatata
     3781 taaatatata ttatacatac tctccttact ttatttcagt taccatcccc atagaatttg
     3841 acaagaattg ctatgactga aaggttttcg agtcctaatt aaaactttat ttatggcagt
     3901 attcataatt agcctgaaat gcattctgta ggtaatctct gagtttctgg aatattttct
     3961 tagacttttt ggatgtgcag cagcttacat gtctgaagtt acttgaaggc atcactttta
     4021 agaaagctta cagttgggcc ctgtaccatc ccaagtcctt tgtagctcct cttgaacatg
     4081 tttgccatac ttttaaaagg gtagttgaat aaatagcatc accattcttt gctgtggcac
     4141 aggttataaa cttaagtgga gtttaccggc agcatcaaat gtttcagctt taaaaaataa
     4201 aagtagggta caagtttaat gtttagttct agaaattttg tgcaatatgt tcataacgat
     4261 ggctgtggtt gccacaaagt gcctcgttta cctttaaata ctgttaatgt gtcatgcatg
     4321 cagatggaag gggtggaact gtgcactaaa gtgggggctt taactgtagt atttggcaga
     4381 gttgccttct acctgccagt tcaaaagttc aacctgtttt catatagaat atatatacta
     4441 aaaaatttca gtctgttaaa cagccttact ctgattcagc ctcttcagat actcttgtgc
     4501 tgtgcagcag tggctctgtg tgtaaatgct atgcactgag gatacacaaa aataccaata
     4561 tgatgtgtac aggataatgc ctcatcccaa tcagatgtcc actcgagaga tctacgggtg
     4621 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     4681 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     4741 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     4801 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     4861 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     4921 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     4981 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     5041 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     5101 gtaggtaacc acgtgcggac cgagcggccg cgaccgcttc ctcgctcact gactcgctgc
     5161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6001 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6061 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6121 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6181 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6241 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6301 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6361 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6421 tggcttcatt caactccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6481 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6541 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6601 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6661 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6721 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6781 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6841 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6901 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6961 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7021 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7081 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7141 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7201 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7261 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccataaaa
     7321 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     7381 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag
     7441 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     7501 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat
     7561 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7621 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7681 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7741 ccgccgcgct taatgcgccg ctacagggcg cgtactatgg ttgctttgac gtatgcggtg
     7801 tgaaataccg cacagatgcg taaggagaaa at
//
