LOCUS       sequence-190068-        8348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNA1
FEATURES             Location/Qualifiers
     source          1..8348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(943..967)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(983..1009)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1022..1045)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1045..1054
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1067..1483)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1083..1099)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1115..1137)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    1550..1891
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             1955..2471
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(2671..2690)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2818..2840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3034..3053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3153..3175
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3213..3231)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3299..3403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3404..3472,3473..4264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3622..3641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4435..5023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4924..4943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5177..5194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5311..5332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5347..5352,5353..5370,5371..5377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5385..5401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             5563..6077
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(6153..6752)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6236..6256)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6733..6752
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6764..7316
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6766..7333
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6926..6945)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7155..7172
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    7346..7370
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(7369..8348,1..943))
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
ORIGIN
        1 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
       61 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
      121 cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
      181 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
      241 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
      301 ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
      361 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
      421 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
      481 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
      541 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctg
      601 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg
      661 ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt
      721 tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc
      781 ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc
      841 ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc
      901 ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgccaact tttttgtaca
      961 aacttgtttg ataacctcga gcagcgtaat ctggaacatc gtatgggtat cctccatcga
     1021 gcttgtcatc gtcatccttg tagtccatgg tggcagatct aattccggcg cctagagaag
     1081 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     1141 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     1201 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     1261 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     1321 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     1381 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     1441 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     1501 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     1561 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     1621 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     1681 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     1741 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     1801 ctaactagta cagacgcagg cgcaaacatt agatgccggc acaaacacgc acaaagacag
     1861 agacagacac gaaacgaccg ctggccagct tacctcccgg cgggggggtc ggtggtccct
     1921 gggcaggggt ctccaaatct cggtggaacc tccaaatgaa agacccccga ggtgggcagt
     1981 caatcaatct gaggagaccc tcccaaggat cagcgagtcc acgattcgga tgcaaacagc
     2041 aagaggcttt attgggaata cgggtacccg ggcgacgcag tctatcggag gactggcgcg
     2101 ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa
     2161 gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg
     2221 gcaccctgga aacatctgat ggttctctag aaactgctga gggcgggacc gcatctgggg
     2281 accatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     2341 cccatattct gctgtctctc tgttcctaac cttgatctga acttctctat tctcagttat
     2401 gtattttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg
     2461 ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt atctatggct
     2521 cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc tggaccactg
     2581 atatcctgtc tttaacaaat tggactaatc gcctctacgc cggacgcatc gtggccggca
     2641 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     2701 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     2761 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcgccgtcgt
     2821 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2881 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2941 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     3001 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3061 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3121 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3181 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3241 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4561 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5161 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5221 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     5281 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5341 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5401 atttcacaat tctccccagc tctttctgcg ctgtccataa tgaactattt caggagtatt
     5461 ggagtatgtg gattcaaaac agtggctaat tgagtcagga ttacttaata ttcgactcct
     5521 accctcagaa tgttgttctc caacttcaag aaactgttac ccatgaaaga cccccgctga
     5581 cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac aagtcggatg
     5641 caactgcaag agggtttatt ggatacacgg gtacccgggc gacgcagtct atcggaggac
     5701 tggcgcgccg agtgaggggt tgtgggctct tttattgagc tcggggagca gaagcgcgcg
     5761 aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt catttcaggt
     5821 ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg cgggaccgca
     5881 tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc acagatatcc
     5941 tgtttggccc atattctgct gtctctctgt tcctaacctt gatctgaact tctctattct
     6001 cagttatgta ttttccatgc cttgcaaaat ggcgttactt aagctagctt gccaaaccta
     6061 caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt ttattttatc
     6121 gataagcttg gctgcaggtc gacatcgatg catcaggcac cgggcttgcg ggtcatgcac
     6181 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     6241 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     6301 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     6361 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     6421 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     6481 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     6541 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     6601 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
     6661 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
     6721 gcgaggcgca ccgtgggctt gtactcggtc atggttgtgg ccatattatc atcgtgtttt
     6781 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     6841 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     6901 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     6961 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     7021 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     7081 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     7141 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     7201 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     7261 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     7321 gggggggggc ggaattcgtt atcgaaccac tttgtacaag aaagttggct cctgcccttc
     7381 ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc
     7441 agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca
     7501 cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc
     7561 atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat
     7621 ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg
     7681 actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta
     7741 aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt
     7801 agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa
     7861 tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt ttttgcgcct
     7921 gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt catctgcggg
     7981 gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac cttcttggtg
     8041 gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac ctggaggggg
     8101 cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg gcctcttttc
     8161 tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg ctctttcacg
     8221 tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac ctcctcgacc
     8281 ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc ctcctgctcc
     8341 tgcccctc
//
