LOCUS       sequence-190069-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-EGFP
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(72..89)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(243..831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(323..342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1002..1793,1794..1862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1625..1644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1863..1967)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1998..2377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2379..2655
                     /label=chicken beta-actin promoter
     intron          2656..3672
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3596..3618
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3680..3699
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3727..3736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3733..3735,3736..3738,3739..4452)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3733..3735,3736..3738,3739..4449)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3778..3799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4039..4058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4386..4407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4484..5072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4537..4557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4955..4966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5162..5181)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5227..5282
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5281..5300)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5643..5659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5643..5659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5656..5678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5667..5683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5691..5697,5698..5715,5716..5721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5736..5757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5846..5879
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6340..8265
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    8433..8466
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8653..10442
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
       61 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1981 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     2041 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2101 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2161 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2221 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2281 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2341 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     2401 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2461 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
     2521 gcggggcgag gggcggggcg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     2581 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     2641 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     2701 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     2761 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     2821 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     2881 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     2941 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     3001 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     3061 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     3121 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     3181 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     3241 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     3301 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     3361 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     3421 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     3481 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     3541 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     3601 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     3661 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     3721 gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3781 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3841 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3901 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3961 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4021 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4081 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4141 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4201 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4261 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4321 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4381 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4441 ctgtacaagt aagaattcaa gcttcgggga ctagtcatat gataatcaac ctctggatta
     4501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4861 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     4921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5041 gagtcggatc tccctttggg ccgcctcccc gcatcggtaa attcactcct caggtgcagg
     5101 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     5161 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     5221 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     5281 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     5341 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     5401 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     5461 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     5521 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     5581 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
     5641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatctcaatt
     5821 ccgatcatat tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
     5881 aggtctgaag aggagtttac gtccagccaa gcttaggatc aattctcatg tttgacagct
     5941 tatcatcgat aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag
     6001 catctctgtc tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac
     6061 cgtccagcaa aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc
     6121 ttgaggatgt tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg
     6181 gttcggagga tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg
     6241 agggtggggg ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg
     6301 tctaataaag atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt
     6361 acaggacctg gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc
     6421 ggcagtggac ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga
     6481 ggacgaggag gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga
     6541 gatggtgtcc ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt
     6601 ggaacaggag caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga
     6661 ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggaggggc aggagcagga
     6721 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
     6781 gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga
     6841 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
     6901 ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca
     6961 ggaggggcag gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggaggggca
     7021 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca
     7081 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga
     7141 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
     7201 ggagcaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg
     7261 gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagc aggaggtgga
     7321 ggccggggtc gaggaggcag tggaggccgg ggtcgaggag gtagtggagg ccggggtcga
     7381 ggaggtagtg gaggccgccg gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa
     7441 agagccaggg ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag
     7501 tcatcatcat ccgggtctcc accgcgcagg ccccctccag gtagaaggcc atttttccac
     7561 cctgtagggg aagccgatta ttttgaatac caccaagaag gtggcccaga tggtgagcct
     7621 gacgtgcccc cgggagcgat agagcagggc cccgcagatg acccaggaga aggcccaagc
     7681 actggacccc ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag
     7741 catcgtggtc aaggaggttc caacccgaaa tttgagaaca ttgcagaagg tttaagagct
     7801 ctcctggcta ggagtcacgt agaaaggact accgacgaag gaacttgggt cgccggtgtg
     7861 ttcgtatatg gaggtagtaa gacctccctt tacaacctaa ggcgaggaac tgcccttgct
     7921 attccacaat gtcgtcttac accattgagt cgtctcccct ttggaatggc ccctggaccc
     7981 ggcccacaac ctggcccgct aagggagtcc attgtctgtt atttcatggt ctttttacaa
     8041 actcatatat ttgctgaggt tttgaaggat gcgattaagg accttgttat gacaaagccc
     8101 gctcctacct gcaatatcag ggtgactgtg tgcagctttg acgatggagt agatttgcct
     8161 ccctggtttc cacctatggt ggaaggggct gccgcggagg gtgatgacgg agatgacgga
     8221 gatgaaggag gtgatggaga tgagggtgag gaagggcagg agtgatgtaa cttgttagga
     8281 gacgccctca atcgtattaa aagccgtgta ttcccccgca ctaaagaata aatccccagt
     8341 agacatcatg cgtgctgttg gtgtatttct ggccatctgt cttgtcacca ttttcgtcct
     8401 cccaacatgg ggcaattgcc ggaaccctta atataacttc gtataatgta tgctatacga
     8461 agttattagg tccctcgaag aggttcacta gcggatctca attgggcata cccatgttgt
     8521 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
     8581 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
     8641 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
     8701 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
     8761 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
     8821 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     8881 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     8941 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
     9001 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
     9061 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
     9121 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     9181 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     9241 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     9301 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
     9361 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
     9421 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
     9481 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
     9541 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
     9601 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
     9661 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
     9721 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
     9781 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
     9841 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
     9901 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
     9961 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
    10021 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
    10081 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
    10141 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
    10201 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
    10261 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
    10321 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
    10381 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
    10441 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
    10501 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
    10561 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
    10621 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
    10681 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
    10741 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
    10801 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
    10861 aggtttagtt cgtccggggc ggggatcgat cctctagagt cgacgctagc g
//
