LOCUS       sequence-19007-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, 
            Sabatini DM
  TITLE     mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms 
            define three distinct mTORC2s.
  JOURNAL   Curr Biol. 2006 Sep 19;16(18):1865-70. doi: 
            10.1016/j.cub.2006.08.001. Epub 2006 Aug 17.
  PUBMED    16919458
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2006.08.001"; journalName: "Curr Biol"; date: 
            "2006-09-19- 19"; volume: "16"; issue: "18"; pages: "1865-70"
COMMENT     Sequence Label: pMSCV-TAP mLST8 (aka: GbL)
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..245
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             246..419
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             456..476
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             483..560
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag attcaggcac catggcaggc cttgcgcaac
       61 acgatgaagc cgtggacaac aaattcaaca aagaacaaca aaacgcgttc tatgagatct
      121 tacatttacc taacttaaac gaagaacaac gaaacgcctt catccaaagt ttaaaagatg
      181 acccaagcca aagcgctaac cttttagcag aagctaaaaa gctaaatgat gctcaggcgc
      241 cgaaagtaga caacaaattc aacaaagaac aacaaaacgc gttctatgag atcttacatt
      301 tacctaactt aaacgaagaa caacgaaacg ccttcatcca aagtttaaaa gatgacccaa
      361 gccaaagcgc taacctttta gcagaagcta aaaagctaaa tgatgctcag gcgccgaaag
      421 tagacgcgaa ttgtgatata cctacaactg cttctgaaaa tttatatttt caaggtgaac
      481 taaagagaag atggaaaaag aatttcatag ccgtctcagc agccaaccgc tttaagaaaa
      541 tctcatcctc cggggcactt ggtgggtggt ggtggtccct cgacgatgaa cacctcccca
      601 ggcacggtgg gcagtgacac ggtcatcctg gccactgcag gctacgacca caccgtgcgc
      661 ttctg
//
