LOCUS       sequence-190091-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP4.2.2
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1833..1850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1839..2063
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2195..2783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2275..2294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3112..3159
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3184..3203
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3391..4185)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3391..4185)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3502..3521)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4112..4131
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4220..4577)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4234..4369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4386..4577
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4534..4554
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4637..5428,5429..5497))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5260..5279
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5498..5602)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5629..6084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5716..5735)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5926..5947
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6096..6475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6476..6679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6630..6650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6724..6743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6724..6742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggccgccat gggttgctct ttttctatct tccttttggc acttctctcg tgcctgactg
       61 tccccgcgtc ggccactaac tatcgcaatg tctcgggcat ctaccacgtc accaatgact
      121 gcccgaactc aagtatagtg tatgaggccg accatcacat cttgcacctc ccaggctgcg
      181 tgccctgcgt gaggacgggg aatcagtcac actgctgggt ggctcttact cctactgtcg
      241 cagcgccata catcggcgca ccgcttgagt ctcttcggag tcatgtggat ttgatggtgg
      301 gaactgccac tgcttgctcg gccctttaca tcggggatct gtgtggtggc ttgttcctgg
      361 ttggtcagat gttctctttc cgaccgcgac gccactggac tacccaggag tgcaattgtt
      421 ccatctatac agggcacctc actggccaca gaatggcttg ggacatgatg atgaactgga
      481 gtccaacaac cactttagtt cttgcccagg tcatgaggat cccaggtact ctggtagacc
      541 tgctcgctgg aggtcactgg ggcgtcctcg tgggagtagc ctatttcagc atgcaagcca
      601 attgggccaa ggtcatctta gtcctattcc tctttgcagg agttgacgcc agcactcaca
      661 tatctggagg gacagctggc cgagctaccg ctggcttcac tgagctcttt acaccaggag
      721 ctaagcaaaa tgtgcagctc attaacagca atgggagttg gcatataaac aggactgccc
      781 tcaactgcaa tgatagttta aacactggat tcctggctgg cctgttctac cactacagct
      841 ttaatagctc agggtgcccc gaacggctcg cgagctgcaa gagccttgac gaccatcacc
      901 aaggctgggg cccactcggg atcgctaaca tcagcggccc gtctaacgac aggccttatt
      961 gctggcacta cgcgcctcgg ccgtgcggga ttgtgccggc atccagtgtg tgtggccccg
     1021 tctactgttt cactcccagc cccgtcgtgg tcggaaccac tgaccgcctc ggggtcccca
     1081 cttacaactg gggggagaat gagactgatg tcttcttact caactcaacc agaccgccgc
     1141 ggggggcatg gtttggatgc gtgtggatga atggcaccgg gtttacaaag acttgtggcg
     1201 cccctccatg cgtggtcaac gccaatacca ataacacttg gcactgcccc accgattgct
     1261 tcaggaagca tccggggacc acttacgcca agtgcgggtc agggccgtgg atcacaccac
     1321 ggtgcttgat tgactacccg taccggctgt ggcatttccc atgtaccatc aattttacca
     1381 tctttaaggt caggacattt gtcggcggga tagagcatcg gatggcagcg gcatgcaact
     1441 ggaccagggg ggaagtctgt ggcttggagc atagggatcg tgcagagtta tcacctctgc
     1501 tcctttccac caccacgtgg caaatcctcc cctgctcttt caccactcta cctgcccttt
     1561 ccaccggctt gatccacctc caccaaaata tcgtggacgt ccagtacctc tacggtgttg
     1621 gatctgcggt ggtatcttgg gccctcaaat gggaatatgt ggtgctcgtg ttcctgcttc
     1681 tcgcggacgc gagagtctcc gcttgcctat ggatgatgtt tatggtaagt caagttgagg
     1741 cgtaatctag aaaccttaag ccgaattctg cagatatcca gcacagtggc ggccgctcga
     1801 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     1861 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1921 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1981 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2041 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagtggcg gtaatacggt
     2101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2761 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2881 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     2941 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     3001 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     3061 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     3121 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     3181 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     3241 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     3301 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     3361 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3421 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3481 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3541 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3601 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     3661 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3721 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3781 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3841 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3901 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3961 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc
     4021 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     4081 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     4141 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     4201 tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4261 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4321 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4381 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4441 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4501 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     4561 ggttctttcc gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt
     4621 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4681 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4741 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4801 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4861 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4921 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4981 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5041 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5101 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5161 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5221 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5281 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5341 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5401 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5461 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5581 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     5641 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5701 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5761 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5821 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5881 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5941 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6001 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6061 acaaaatatt aacgcttaca atttacgcgc gcgttgacat tgattattga ctagttatta
     6121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     6181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     6241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     6301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     6361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     6421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     6661 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     6721 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
     6781 accgagctcg gatccactag tccagtgtgg tggaattcgg cttacag
//
