LOCUS       sequence-190095-       10844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter to monitor translation of 5'TOP mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Han K, Jaimovich A, Dey G, Ruggero D, Meyuhas O, Sonenberg N, Meyer 
            T
  TITLE     Parallel measurement of dynamic changes in translation rates in 
            single cells.
  JOURNAL   Nat Methods. 2014 Jan;11(1):86-93. doi: 10.1038/nmeth.2729. Epub 
            2013 Nov 10.
  PUBMED    24213167
REFERENCE   2  (bases 1 to 10844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2729"; journalName: "Nat Methods"; date: "2014-01"; 
            volume: "11"; issue: "1"; pages: "86-93"
COMMENT     Sequence Label: TOP-H2B-YFP-DD
FEATURES             Location/Qualifiers
     source          1..10844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1163
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1154
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1111..1131
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1221..1598
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(1605..1607,1608..2318)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1647..1668)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1908..1927)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2255..2276
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2325..2801
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    2955..3543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3008..3028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3426..3437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4062..4242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4268..4285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4274..4498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4544..4972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4631..4650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4841..4862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4981..5001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4986..5315
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5166..5301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5228..5247
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5363..5410
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5429..5803
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5933..6054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5970..5989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6024..6043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6103..6119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6103..6119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6116..6138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6127..6143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6151..6157,6158..6175,6176..6181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6196..6217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6334..6351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6505..7093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6585..6604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7264..8055,8056..8124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7887..7906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8125..8229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8304..8323)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8495..8874
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8876..9074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9029..9049
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9092..9272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9319..9444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9936..10169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10354..10398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10672..10789
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg ggcgggcctt
      361 gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
      421 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
      481 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
      541 atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
      661 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
      721 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
      781 gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg cgctcgggag
      841 agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca gccgtcgctt
      901 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tggagctttt
      961 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     1021 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     1081 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     1141 ttcttccatt tcaggtgtcg tgaacacgct accggtctcg agctcaagct tcgaattctg
     1201 cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg
     1261 gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc
     1321 gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg
     1381 gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca
     1441 tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg
     1501 agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg
     1561 agggtactaa ggccatcacc aagtacacca gcgctaagga tccagtgagc aagggcgagg
     1621 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1681 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1741 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     1801 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1861 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1921 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1981 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2041 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2101 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2161 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     2221 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2281 ccgccgggat cactctcggc atggacgagc tgtacaagct cgagatcagt ctgattgcgg
     2341 cgttagcggt agatcacgtt atcggcatgg aaaccgtcat gccgtggaac ctgcctgccg
     2401 atctcgcctg gtttaaacgc aacaccttaa ataaacccgt gattatgggc cgccatacct
     2461 gggaatcaat cggtcgtccg ttgccaggac gcaaaaatat tatcctcagc agtcaaccga
     2521 gtacggacga tcgcgtaacg tgggtgaagt cggtggatga agccatcgcg gcgtgtggtg
     2581 acgtaccaga aatcatggtt attggcggcg gtcgcgttta tgaacagttc ttgccaaaag
     2641 cgcaaaaact gtatctgacg catatcgacg cagaagtgga aggcgacacc catttcccgg
     2701 attacgagcc ggatgactgg gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga
     2761 actctcacag ctattgcttt gagattctgg agcggcgatg actgcaggcg gccgcgactc
     2821 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa catcgaggga
     2941 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3001 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3061 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3121 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3181 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3241 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3301 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     3361 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3421 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3481 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3541 cgcatcgata ccgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     3601 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     3661 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3721 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     3781 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     3841 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     3901 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     3961 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     4021 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     4081 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4141 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4201 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     4261 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4501 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4561 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4621 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4681 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4741 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4801 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4861 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4921 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4981 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5041 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5161 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5221 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5281 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5341 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     5401 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     5461 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     5521 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     5581 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     5641 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     5701 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     5761 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     5821 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5881 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     5941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6061 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8281 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8341 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8401 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8461 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     8521 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     8581 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8641 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8701 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     8761 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     8821 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     8881 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     8941 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     9001 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     9061 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     9121 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9181 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9241 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9301 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9361 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9421 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9481 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9541 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9601 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9661 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9721 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     9781 gggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9841 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9901 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9961 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    10021 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    10081 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    10141 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    10201 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    10261 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    10321 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    10381 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    10441 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    10501 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    10561 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    10621 agatccattc gattagtgaa cggatctaca aatggcagta ttcatccaca attttaaaag
    10681 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    10741 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    10801 cagggacagc agaaattcac tttgaattaa ttcaagcttc gtga
//
