LOCUS       sequence-190098-        9377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter to monitor translation of unstructured mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Han K, Jaimovich A, Dey G, Ruggero D, Meyuhas O, Sonenberg N, Meyer 
            T
  TITLE     Parallel measurement of dynamic changes in translation rates in 
            single cells.
  JOURNAL   Nat Methods. 2014 Jan;11(1):86-93. doi: 10.1038/nmeth.2729. Epub 
            2013 Nov 10.
  PUBMED    24213167
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2729"; journalName: "Nat Methods"; date: "2014-01"; 
            volume: "11"; issue: "1"; pages: "86-93"
COMMENT     Sequence Label: UnReg-H2B-YFP-DD
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     123..142
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             186..563
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             join(570..572,573..1283)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(612..633)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(873..892)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1220..1241
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1290..1766
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    complement(1781..1805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1836..2424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1889..1909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2307..2318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2438..2937
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2473..2496)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2870..2889
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2958..3557
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2958..2977)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3454..3474
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3695..3928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4000..4121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4037..4056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4091..4110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4161..4296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4316..4335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4317..4335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4345..4362)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4345..4361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4354..4376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4503..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4590..4609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4800..4821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4984..5088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5089..5157,5158..5949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5307..5326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6120..6708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6609..6628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6862..6879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6996..7017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7032..7037,7038..7055,7056..7062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7070..7086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7075..7097
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7094..7110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7094..7110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7129..7149
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7131..7149
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7177..7403
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7404..7584
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7631..7756
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8249..8482
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8667..8711
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8978..9094
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9128..9152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
       61 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      121 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgc tcgagaccta
      181 ggaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
      241 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
      301 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
      361 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
      421 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
      481 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
      541 tcaccaagta caccagcgct aaggatccag tgagcaaggg cgaggagctg ttcaccgggg
      601 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      661 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
      721 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
      781 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      841 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      901 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      961 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1021 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1081 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1141 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     1201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1261 tcggcatgga cgagctgtac aaggtcgaga tcagtctgat tgcggcgtta gcggtagatc
     1321 acgttatcgg catggaaacc gtcatgccgt ggaacctgcc tgccgatctc gcctggttta
     1381 aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa tcaatcggtc
     1441 gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgagtacg gacgatcgcg
     1501 taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta ccagaaatca
     1561 tggttattgg cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc
     1621 tgacgcatat cgacgcagaa gtggaaggcg acacccattt cccggattac gagccggatg
     1681 actgggaatc ggtattcagc gaattccacg atgctgatgc gcagaactct cacagctatt
     1741 gctttgagat tctggagcgg cgatgagtcg agatatctag acccagcttt cttgtacaaa
     1801 gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     1861 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     1921 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     1981 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2041 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2101 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2161 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     2221 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     2281 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     2341 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     2401 tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca
     2461 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     2521 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     2581 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     2641 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     2701 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     2761 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     2821 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     2881 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     2941 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     3001 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     3061 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     3121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     3181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     3241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     3301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     3361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     3421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     3481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     3541 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     3601 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
     3661 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
     3721 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3901 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     3961 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     4021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4081 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     4141 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4261 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     4321 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4381 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4441 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4501 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4861 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4921 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6781 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6841 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6901 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6961 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7021 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7081 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     7141 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     7201 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     7261 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     7321 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     7381 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     7441 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7501 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7561 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7621 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7681 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7741 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7801 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7861 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7921 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7981 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8041 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8101 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8161 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8221 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8281 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8341 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8401 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8461 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8521 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8581 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8641 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8701 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8761 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8821 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8881 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8941 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     9001 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9061 aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca
     9121 gatatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag tcgacattga
     9181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     9241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     9301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     9361 tgacgtcaat gggtgga
//
