LOCUS       sequence-190099-        7251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP5.1.1
FEATURES             Location/Qualifiers
     source          1..7251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1802..1843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1853..1870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1893..1910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1899..2123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2169..2597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2256..2275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2466..2487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2606..2626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2611..2940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2791..2926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2853..2872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3007..3801
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3061..3080)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3671..3690
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3977..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4014..4033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4068..4087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4147..4163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4147..4163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4160..4182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4171..4187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4195..4201,4202..4219,4220..4225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4240..4261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4378..4395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4549..5137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4629..4648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5308..6099,6100..6168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5931..5950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6169..6273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6348..6367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6539..6918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6919..7122
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7073..7093
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7167..7186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7167..7185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctttctcta tctttatcct tgcacttctc tcgtgcctga ccgtcccagc ctctgcagtc
       61 ccctaccgga atgcctctgg gatctaccac gtcaccaatg attgcccaaa ctcttccata
      121 gtatatgagg cagataacct gattttacac gcacccggct gcgtgccctg tgttagggag
      181 ggtaatgtga gtaggtgctg ggtccaaatc acccccacac tgtcggcccc aaacctcgga
      241 gcggtcacgg ctcctcttcg gagagccgtt gactacttag cgggaggggc cgccctttgc
      301 tccgcgttat acgtcgggga cgcgtgcggg gcggtgttct tggtaggcca aatgttcacc
      361 tataggcctc gccagcatac tacggtgcag gactgcaact gttctattta cagtggccat
      421 gttagcggcc accggatggc atgggacatg atgatgaatt ggtcacctac atcggccttg
      481 ctggtagccc agatgctacg gatccctcaa gtggtcatgg acatcatagc cgggggccac
      541 tggggggtct tgttcgccgc cgcgtacttt gcatcagcgg ctaactgggc taaggttgtg
      601 ctggttctgt tcttgtttgc gggagttgac ggtaacaccc agactgtggg cggttctgct
      661 gcttattcta catcaggcct ggcctcgctc ttcagccccg ggccaaagca gaacctccag
      721 ctcatacaaa atgggagcag ctggcacatt aacaggactg cccttaactg taatgacagc
      781 ctcaagactg ggttcatagc cggcctcatg tacgtctata agttcaactc caccgggtgt
      841 ccggagcgga tggctagttg tagacccctc acagccttcg accagggctg gggaactatc
      901 agctatgcca acatatcggg ccctagtgac gacaaaccat attgctggca ctatccccca
      961 cgaccgtgcg gagtggtgcc agcccgagag gtctgcggtc cagtctactg tttcacacct
     1021 agcccagtgg tcgttggcac cactgatcgc aaaggatatc ccacttacaa ctggggtgaa
     1081 aatgacactg acgtccttct cttgaacaac acgaggcctc ccactggcaa ttggtttggc
     1141 tgcacctgga tgaacgcctc agggtttgtg aagacttgcg gagcgccacc ttgcatctta
     1201 gggccgacag gcaacaacag tcttaagtgc cctacagatt gcttccgcaa gcacccggac
     1261 gccacctaca ccaggtgcgg gtcaggaccc tggcttacgc cccggtgttt ggtggactac
     1321 ccttaccggt tgtggcatta cccgtgcacc gtaaattata ccatctttaa ggtgcgcatg
     1381 ttcattgggg gcctagagca caggtttgat gcggcatgta actggacccg aggtgaacga
     1441 tgcgacctcg atgataggga tagggccgag ctaagcccgc ttctacacac taccacacag
     1501 tgggccatat tgccgtgctc ttttacaccc acgcctgctc ttagtactgg cctcattcac
     1561 ttacaccaga atatagtaga cacccagtat ctttacggcc tgagctccag catcgtctcg
     1621 tgggccgtta agtgggagta catagtgctg gccttcctgc tacttgctga tgcccgtgtc
     1681 tgtacttgcc tatggatcat gctcctagtc tgtcaggctg aagcataaaa agggtcaaga
     1741 caattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     1801 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     1861 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     1921 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1981 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2041 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2101 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     2161 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2221 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2281 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2341 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2401 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2461 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2521 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2581 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2761 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     2881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2941 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     3001 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3061 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3121 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3181 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3241 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3301 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3361 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3421 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3481 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3541 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3601 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3661 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3721 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3781 cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac
     3841 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3901 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3961 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4021 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4081 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4141 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4201 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4261 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4981 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6301 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     6361 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     6421 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     6481 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     6541 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     6601 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6661 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6721 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6781 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6841 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6901 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6961 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     7021 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     7081 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     7141 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
     7201 tagttaagct tggtaccgag ctcggatcca gtacccttca ccatgggttg c
//
