LOCUS       sequence-190100-         511 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the catalytically inactive version of ThermoCas9 
            (Thermo(d)Cas9) and its sgRNA module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, 
            Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der 
            Oost J
  TITLE     Characterizing a thermostable Cas9 for bacterial genome editing and 
            silencing.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4.
  PUBMED    29162801
REFERENCE   2  (bases 1 to 511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1647. doi"; pages: " 
            10.1038/s41467-017-01591-4"
COMMENT     Sequence Label: pThermoCas9i_ctrl
FEATURES             Location/Qualifiers
     source          1..511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgccggaat tctttcacag ccgcatccct ttcttccgca ggcagcgcct tccagcccgc
       61 ccagtcgaga gaacggaaat catgcagtga gtaccagcca tctagtgttt ctgctgcttc
      121 gctcattttt ctccctccaa atgtttttct cttcccttac tataacacag tttagaagag
      181 ggagaaaata ttacggctgc cgcgaaattg tctaaaagaa gccttctttt gcctttgatt
      241 ttccggaaaa atggattatt cttaaaatgg ataccgttat acggatccca tgacgctagt
      301 atccagctgg gtcatagttc ccctgagatt atcgctgtgg tataatgaaa gttataccac
      361 agcaatgatc tcagggttac tatgataagg gctttctgcc tataggcaga ctgacccgtg
      421 gcgttgggga tcgcctatcg cccgctttct tcgggcattc cccactctta ggcgaacgaa
      481 gaccttggcg gaaaacgtca aggtcttttt t
//
