LOCUS       sequence-190102-        3549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the catalytically inactive version of ThermoCas9 
            (Thermo(d)Cas9) and its sgRNA module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Mougiakos I, Mohanraju P, Bosma EF, Vrouwe V, Finger Bou M, 
            Naduthodi MIS, Gussak A, Brinkman RBL, van Kranenburg R, van der 
            Oost J
  TITLE     Characterizing a thermostable Cas9 for bacterial genome editing and 
            silencing.
  JOURNAL   Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4.
  PUBMED    29162801
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-21"; volume: "8(1):1647. doi"; pages: " 
            10.1038/s41467-017-01591-4"
COMMENT     Sequence Label: pThermoCas9i_ctrl
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaacatcctc tttcttagtt tgtatagtca ataaacgaac taaattttca tttttatctt
       61 tttctagaca tttttattgt atcgtatttt ttattcaaaa aaaatattga attttaaaaa
      121 tgatggtgca gtatgaaggt gtacttagtt tattggttaa acaaagtacg catcaatata
      181 ttttttttga tgcgatttga attcgctttc attatctaaa acattcaatc gaggaggtaa
      241 cttaaatgaa gtataaaatc ggtcttgcta tcggcattac gtctatcggt tgggctgtca
      301 ttaatttgga cattcctcgc atcgaagatt taggtgtccg catttttgac agagcggaaa
      361 acccgaaaac cggggagtca ctagctcttc cacgtcgcct cgcccgctcc gcccgacgtc
      421 gtctgcggcg tcgcaaacat cgactggagc gcattcgccg cctgttcgtc cgcgaaggaa
      481 ttttaacgaa ggaagagctg aacaagctgt ttgaaaaaaa gcacgaaatc gacgtctggc
      541 agcttcgtgt tgaagcactg gatcgaaaac taaataacga tgaattagcc cgcatccttc
      601 ttcatctggc taaacggcgt ggatttagat ccaaccgcaa gagtgagcgc accaacaaag
      661 aaaacagtac gatgctcaaa catattgaag aaaaccaatc cattctttca agttaccgaa
      721 cggttgcaga aatggttgtc aaggatccga aattttccct gcacaagcgt aataaagagg
      781 ataattacac caacactgtt gcccgcgacg atcttgaacg ggaaatcaaa ctgattttcg
      841 ccaaacagcg cgaatatggg aacatcgttt gcacagaagc atttgaacac gagtatattt
      901 ccatttgggc atcgcaacgc ccttttgctt ctaaggatga tatcgagaaa aaagtcggtt
      961 tctgtacgtt tgagcctaaa gaaaaacgcg cgccaaaagc aacatacaca ttccagtcct
     1021 tcaccgtctg ggaacatatt aacaaacttc gtcttgtctc cccgggaggc atccgggcac
     1081 taaccgatga tgaacgtcgt cttatataca agcaagcatt tcataaaaat aaaatcacct
     1141 tccatgatgt tcgaacattg cttaacttgc ctgacgacac ccgttttaaa ggtcttttat
     1201 atgaccgaaa caccacgctg aaggaaaatg agaaagttcg cttccttgaa ctcggcgcct
     1261 atcataaaat acggaaagcg atcgacagcg tctatggcaa aggagcagca aaatcatttc
     1321 gtccgattga ttttgataca tttggctacg cattaacgat gtttaaagac gacaccgaca
     1381 ttcgcagtta cttgcgaaac gaatacgaac aaaatggaaa acgaatggaa aatctagcgg
     1441 ataaagtcta tgatgaagaa ttgattgaag aacttttaaa cttatcgttt tctaagtttg
     1501 gtcatctatc ccttaaagcg cttcgcaaca tccttccata tatggaacaa ggcgaagtct
     1561 actcaaccgc ttgtgaacga gcaggatata catttacagg gccaaagaaa aaacagaaaa
     1621 cggtattgct gccgaacatt ccgccgatcg ccaatccggt cgtcatgcgc gcactgacac
     1681 aggcacgcaa agtggtcaat gccattatca aaaagtacgg ctcaccggtc tccatccata
     1741 tcgaactggc ccgggaacta tcacaatcct ttgatgaacg acgtaaaatg cagaaagaac
     1801 aggaaggaaa ccgaaagaaa aacgaaactg ccattcgcca acttgttgaa tatgggctga
     1861 cgctcaatcc aactgggctt gacattgtga aattcaaact atggagcgaa caaaacggaa
     1921 aatgtgccta ttcactccaa ccgatcgaaa tcgagcggtt gctcgaacca ggctatacag
     1981 aagtcgacgc tgtgattcca tacagccgaa gcttggacga tagctatacc aataaagttc
     2041 ttgtgttgac aaaggagaac cgtgaaaaag gaaaccgcac cccagctgaa tatttaggat
     2101 taggctcaga acgttggcaa cagttcgaga cgtttgtctt gacaaataag cagttttcga
     2161 aaaagaagcg ggatcgactc cttcggcttc attacgatga aaacgaagaa aatgagttta
     2221 aaaatcgtaa tctaaatgat acccgttata tctcacgctt cttggctaac tttattcgcg
     2281 aacatctcaa attcgccgac agcgatgaca aacaaaaagt atacacggtc aacggccgta
     2341 ttaccgccca tttacgcagc cgttggaatt ttaacaaaaa ccgggaagaa tcgaatttgc
     2401 atcatgccgt cgatgctgcc atcgtcgcct gcacaacgcc gagcgatatc gcccgagtca
     2461 ccgccttcta tcaacggcgc gaacaaaaca aagaactgtc caaaaagacg gatccgcagt
     2521 ttccgcagcc ttggccgcac tttgctgatg aactgcaggc gcgtttatca aaaaatccaa
     2581 aggagagtat aaaagctctc aatcttggaa attatgataa cgagaaactc gaatcgttgc
     2641 agccggtttt tgtctcccga atgccgaagc ggagcataac aggagcggct catcaagaaa
     2701 cattgcggcg ttatatcggc atcgacgaac ggagcggaaa aatacagacg gtcgtcaaaa
     2761 agaaactatc cgagatccaa ctggataaaa caggtcattt cccaatgtac gggaaagaaa
     2821 gcgatccaag gacatatgaa gccattcgcc aacggttgct tgaacataac aatgacccaa
     2881 aaaaggcgtt tcaagagcct ctgtataaac cgaagaagaa cggagaacta ggtcctatca
     2941 tccgaacaat caaaatcatc gatacgacaa atcaagttat tccgctcaac gatggcaaaa
     3001 cagtcgccta caacagcaac atcgtgcggg tcgacgtctt tgagaaagat ggcaaatatt
     3061 attgtgtccc tatctataca atagatatga tgaaagggat cttgccaaac aaggcgatcg
     3121 agccgaacaa accgtactct gagtggaagg aaatgacgga ggactataca ttccgattca
     3181 gtctataccc aaatgatctt atccgtatcg aatttccccg agaaaaaaca ataaagactg
     3241 ctgtggggga agaaatcaaa attaaggatc tgttcgccta ttatcaaacc atcgactcct
     3301 ccaatggagg gttaagtttg gttagccatg ataacaactt ttcgctccgc agcatcggtt
     3361 caagaaccct caaacgattc gagaaatacc aagtagatgt gctaggcaac atctacaaag
     3421 tgagagggga aaagagagtt ggggtggcgt catcttctca ttcgaaagcc ggggaaacta
     3481 tccgtccgtt ataaatcaga caaaatggcc tgcttatgaa gcgggccatt tttgtttaat
     3541 cctgtcgac
//
