LOCUS       sequence-190104-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter to monitor translation of 5'TOP mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Han K, Jaimovich A, Dey G, Ruggero D, Meyuhas O, Sonenberg N, Meyer 
            T
  TITLE     Parallel measurement of dynamic changes in translation rates in 
            single cells.
  JOURNAL   Nat Methods. 2014 Jan;11(1):86-93. doi: 10.1038/nmeth.2729. Epub 
            2013 Nov 10.
  PUBMED    24213167
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2729"; journalName: "Nat Methods"; date: "2014-01"; 
            volume: "11"; issue: "1"; pages: "86-93"
COMMENT     Sequence Label: TOP-H2B-mCherry-DD
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1163
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1154
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1111..1131
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1221..1598
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             1605..2309
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1747..1765)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2015..2034
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2316..2792
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    2940..3528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2993..3013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3411..3422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4047..4227
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4253..4270)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4259..4483
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4529..4957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4616..4635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4826..4847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4966..4986)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4971..5300
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5151..5286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5213..5232
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5348..5395
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5414..5788
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5918..6039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5955..5974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6009..6028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6088..6104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6088..6104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6101..6123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6112..6128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6136..6142,6143..6160,6161..6166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6181..6202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6319..6336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6490..7078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6570..6589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7249..8040,8041..8109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7872..7891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8110..8214)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8289..8308)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8480..8859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8861..9059
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9014..9034
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9077..9257
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9304..9429
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9921..10154
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10339..10383
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10657..10774
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg ggcgggcctt
      361 gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
      421 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
      481 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
      541 atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
      601 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
      661 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
      721 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
      781 gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg cgctcgggag
      841 agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca gccgtcgctt
      901 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tggagctttt
      961 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     1021 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     1081 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     1141 ttcttccatt tcaggtgtcg tgaacacgct accggtctcg agctcaagct tcgaattctg
     1201 cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg
     1261 gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc
     1321 gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg
     1381 gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca
     1441 tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg
     1501 agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg
     1561 agggtactaa ggccatcacc aagtacacca gcgctaagga tccagtgagc aagggcgagg
     1621 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1681 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1741 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1801 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1861 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1921 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1981 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2041 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     2101 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     2161 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     2221 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     2281 ccaccggcgg catggacgag ctgtacaagg tcgagatcag tctgattgcg gcgttagcgg
     2341 tagatcacgt tatcggcatg gaaaccgtca tgccgtggaa cctgcctgcc gatctcgcct
     2401 ggtttaaacg caacacctta aataaacccg tgattatggg ccgccatacc tgggaatcaa
     2461 tcggtcgtcc gttgccagga cgcaaaaata ttatcctcag cagtcaaccg agtacggacg
     2521 atcgcgtaac gtgggtgaag tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag
     2581 aaatcatggt tattggcggc ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac
     2641 tgtatctgac gcatatcgac gcagaagtgg aaggcgacac ccatttcccg gattacgagc
     2701 cggatgactg ggaatcggta ttcagcgaat tccacgatgc tgatgcgcag aactctcaca
     2761 gctattgctt tgagattctg gagcggcgat gagcggccgc gactctagat cataatcagc
     2821 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2881 ctgaaacata aaatgaatgc aattgttgtt gttaacatcg agggatcaag cttatcgata
     2941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3301 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3541 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     3601 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     3661 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3721 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     3781 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     3841 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     3901 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     3961 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     4021 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4081 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4141 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4201 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     4261 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4321 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4381 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4441 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4501 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4681 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4861 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4921 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4981 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5041 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5101 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5161 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5221 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5281 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5341 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     5401 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     5461 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     5521 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     5581 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     5641 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     5701 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     5761 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     5821 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5881 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6061 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8221 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     8281 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     8341 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     8401 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     8461 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     8521 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     8581 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     8641 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     8701 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     8761 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     8821 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     8881 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     8941 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9001 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     9061 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9121 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9181 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9241 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     9301 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     9361 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9421 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9481 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9541 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     9601 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     9661 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     9721 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagggcc gctgatcttc
     9781 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     9841 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     9901 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9961 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    10021 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    10081 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    10141 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    10201 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    10261 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    10321 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    10381 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    10441 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    10501 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    10561 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    10621 gtgaacggat ctacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    10681 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    10741 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaaa
    10801 ttcactttga attaattcaa gcttcgtga
//
