LOCUS       sequence-190105-        6807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP5.2.1
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1813..1830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1819..2043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2175..2763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2255..2274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3092..3139
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3164..3183
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3371..4165)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3371..4165)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3482..3501)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4092..4111
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4200..4557)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4214..4349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4366..4557
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4514..4534
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4617..5408,5409..5477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5240..5259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5478..5582)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5609..6064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5696..5715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5906..5927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6076..6455
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6456..6659
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6610..6630
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6704..6723
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6704..6722
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgggttgct ctttctctat cttcatcctt gcacttctct cgtgcttgac cgtcccggcc
       61 tctgcagttc cctaccgaaa tgcctctggg gtctatcacg tcaccaatga ttgcccaaac
      121 tcttccatag tttacgaggc taatgacctg atcttacacg cacctggttg tgtgccttgt
      181 gttaggacag gtaatgtcag taggtgctgg gtccagatca cccccacgct gtcagccccg
      241 agccttggag caatcacggc ccctcttcgg agggccgtcg actaccttgc gggaggggct
      301 gccctttgct ccgcgttata cgtcggagac gcgtgtgggg cagtgttctt ggtaggccaa
      361 atgttcacct ataggcctcg ccagcatgct acggtgcagg actgcaactg ttctatctac
      421 agtggccacg tcaccggcca ccggatggca tgggacatga tgatgaattg gtcacccacg
      481 acagccttgc tgatggccca gttgctacgg atcccccagg tggtcgttga catcatcgcc
      541 ggcggccact ggggggtctt gttcgccgcc gcatactttg cgtcagcggc taactgggct
      601 aaggttgtgc tggtcttgtt cctgttcgca ggggttgatg ccgaaaccca ctcagtgggc
      661 ggtgttgtcg cccgcgactt gcgcagcctg accagcattt tcacccccgg gccgcggcag
      721 aacttgcagc tcgtgaacac caacgggagc tggcacatca acagaactgc tcttaattgt
      781 caagacagcc tccagactgg gtttatagct ggcctcctgt acttcaacaa gttcaactcc
      841 tctgggtgtc cagagcggat ggctagctgc aggcccctta ctgcatttga ccagggctgg
      901 ggaaccatca gctatgctga catgtcgggc cccagcgatg acaagccata ctgctggcac
      961 tatcccccac gaccgtgcgg agtggtgcca gcccaaagcg tctgtggccc agtctactgt
     1021 tttacaccca gcccagtggt cgtcggcacc actgaccgca gagggtatcc cacttacaac
     1081 tggggcagca atgagactga cgtcattctc ttgaacagca cgaggccccc tgctggcagt
     1141 tggtttggct gtacttggat gaacgccaca gggtttgtga agacttgcgg agccccgccc
     1201 tgcaacttag ggccgacagg caacaccagc cttaagtgtc ctactgattg cttccggaag
     1261 cacccagaag ccacctacac taggtgcggg tcagggccct ggctcacccc ccggtgtttg
     1321 gtggattacc cttacaggtt gtggcattat ccgtgcaccg taaactatac catcttcaag
     1381 gtgcgcatgt acattggagg cttggagcac aggctcgatg cagcttgcaa ctggacccgc
     1441 ggtgagcgat gtgatctcga agacagagat agggccgagc tgagcccgct cctgcacacc
     1501 accacacagt gggccatatt gccgtgctct ttcacaccca cgcctgctct tagcactggc
     1561 ctcatacact tacatcaaaa tatagtagac acccagtatc tttacggcct gagctccagc
     1621 atcatctcgt gggccgttaa gtgggagtat gtagtactgg cctttttact actcgctgat
     1681 gcccgtatct gtacctgcct atggatcatg ctcctagttt gccaggctga agcataaaag
     1741 ccgaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     1801 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1861 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1921 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1981 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2041 tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga atcaggggat
     2101 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2161 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2221 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2281 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2341 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2401 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2461 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2521 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2581 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2641 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2701 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2761 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2821 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2881 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct
     2941 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
     3001 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
     3061 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta
     3121 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     3181 tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     3241 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     3301 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     3361 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     3421 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     3481 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     3541 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     3601 catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     3661 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     3721 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     3781 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     3841 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     3901 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     3961 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     4021 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     4081 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     4141 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     4201 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     4261 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     4321 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     4381 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     4441 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     4501 ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcaggac
     4561 tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4741 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4921 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5221 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5581 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5641 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5701 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5761 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5821 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5881 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5941 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6001 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6061 atttacgcgc gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     6121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     6181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     6241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     6301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     6361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     6421 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     6481 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     6541 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     6601 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     6661 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
     6721 gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg gatccactag
     6781 tccagtgtgg tggaattcgg cttcacc
//
