LOCUS       sequence-190106-        9580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with 
            Dam-V5 using Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw EcoDam-V5-NKX2-5Y191C
FEATURES             Location/Qualifiers
     source          1..9580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..2008
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1955..1976
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2903..2944
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2960..2984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3996..4020)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             4551..4731
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4757..4774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4763..4987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5033..5461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5470..5490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5475..5804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5655..5790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5717..5736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5852..5899
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5918..6292
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6422..6543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6459..6478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6513..6532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6592..6608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6592..6608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6605..6627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6616..6632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6640..6646,6647..6664,6665..6670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6685..6706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6823..6840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6994..7582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7074..7093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7753..8544,8545..8613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8376..8395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8614..8718)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8793..8812)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8984..9363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9365..9563
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9518..9538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatccccc
     1561 gggctgcagg aattcagatc tcggccgcat attaagtgca ttgttctcga taccgctaag
     1621 tgcattgttc tcgttagctc gatggacaag tgcattgttc tcttgctgaa agctcgatgg
     1681 acaagtgcat tgttctcttg ctgaaagctc gatggacaag tgcattgttc tcttgctgaa
     1741 agctcagtac ccgggagtac cctcgaccgc cggagtataa atagaggcgc ttcgtctacg
     1801 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
     1861 taaacaagcg cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc
     1921 aattaaaagt aaccagcaac caagtaaatc aactgcaact actgaaatct gccaagaagt
     1981 aattattgaa tacaagaaga gaactctgaa tactttcaac aagttaccga gaaagaagaa
     2041 ctcacacaca gctagcgcca ccatgaagaa aaatcgcgct tttttgaagt gggcaggggg
     2101 caagtatccc ctgcttgatg atattaaacg gcatttgccc aagggcgaat gtctggttga
     2161 gccttttgta ggtgccgggt cggtgtttct caacaccgac ttttctcgtt atatccttgc
     2221 cgatatcaat agcgacctga tcagtctcta taacattgtg aagatgcgta ctgatgagta
     2281 cgtacaggcc gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg tttactatca
     2341 gttccgcgaa gagttcaaca aaagccagga tccgttccgt cgggcggtac tgtttttata
     2401 tttgaaccgc tacggttaca acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt
     2461 gccgttcggc cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga
     2521 aaaagcgcag aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga
     2581 tgatgcatcc gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt
     2641 tacggcgtat cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc
     2701 cgaaggtctg gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac
     2761 gcgtgagtgg tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag
     2821 caacggcggc acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt
     2881 ttcacccgcg aaaaaaaccg gtggtaagcc tatccctaac cctctcctcg gtctcgattc
     2941 tacgatcgat ttcgatcaaa caagtttgta caaaaaagca ggctggcgcc ggaaccaatt
     3001 cagtcgactg gatccgatgt tccccagccc tgcgctcaca cccacgcctt tctcagtcaa
     3061 agacatcctg aacctggagc agcagcagcg tagcctggcg tctggggacc tgtctgcgcg
     3121 cctcgaggcc accctggccc ctgcctcctg catgctggcc gccttcaagc ccgaggccta
     3181 ctctggcccc gaggcggcag cgtccggcct ggcagagctg cgcgcggaga tgggccccgc
     3241 gccttcgccc cccaagtgct ctcctgcttt cccagccgcc cccacatttt acccgggagc
     3301 ctacggtgac cctgacccag ccaaagaccc tcgggcggat aaaaaagagc tgtgcgcgct
     3361 gcagaaggca gtggagctgg acaaagccga gacggatggc gccgagagac cacgcgcacg
     3421 gcggcgacgg aagccacgcg tgctcttctc gcaggcgcag gtctacgagc tggagcggcg
     3481 cttcaagcaa cagcggtacc tgtcggcgcc agagcgcgac cagctggcca gcgtgctgaa
     3541 gctcacgtcc acgcaggtca agatctggtt ccagaaccgt cgctgcaagt gcaagcgaca
     3601 gcggcaggac cagactctgg agcttctggg gccgccgccg ccgcccgcgc gcaggatcgc
     3661 ggtgcccgtg ctggtgcgcg acgggaagcc ctgcctgggg gaccccgcgg cctacgctcc
     3721 cgcctacggc gtgggtctca atgcctatgg ctacaacgcc tacccctacc ccagctacgg
     3781 cggcgcggcc tgcagtcccg gctacagctg cgccgcctac cccgctgcgc cccccgccgc
     3841 gcagcccccc gccgcctccg ccaacagcaa cttcgtgaac tttggcgtcg gggacttgaa
     3901 caccgtgcag agtcccggga tgccgcaggg caattcgggc gtctccacgc tgcacggcat
     3961 ccgagcctgg tagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt
     4021 tcgatcgaac cgcggtctag actcgagacc tagaaaaaca tggagcaatc acaagtagca
     4081 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     4141 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     4201 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4261 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     4321 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     4381 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     4441 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     4501 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     4561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     4741 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4801 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4861 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4921 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4981 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5521 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5641 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5701 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5761 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5821 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     5881 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     5941 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     6001 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     6061 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     6121 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     6181 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     6241 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     6301 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6361 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6421 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6541 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8761 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8821 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8881 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8941 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     9001 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     9061 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     9241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     9301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     9361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     9421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     9481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     9541 cggtgggagg tctatataag cagcgcgttt tgcctgtact
//
