LOCUS       sequence-190107-        6819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP6.1.1
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1830..1847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1836..2060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2192..2780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2272..2291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3109..3156
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3181..3200
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3388..4182)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3388..4182)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3499..3518)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4109..4128
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4217..4574)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4231..4366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4383..4574
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4531..4551
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4634..5425,5426..5494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5257..5276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5495..5599)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5626..6081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5713..5732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5923..5944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6093..6472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6473..6676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6627..6647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6721..6740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6721..6739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaccatggg ttgctctttc tctatcttcc ttttggcact actctcgtgc ctcacaacgc
       61 cggcttcggc tcttacctac ggtaactcca gtgggctgta ccatcttaca aatgattgcc
      121 ccaactccag catcgtgctg gaagcggatg ctatgatctt gcatctgcct ggatgcttgc
      181 cttgtgtgag ggtcgataat cggtccacct gctggcatgc tgtgtccccc accctagcca
      241 taccaaatgc ttctacgccc gcaacgggat tccgcaggca tgtggatctt cttgcgggcg
      301 ccgcggtggt ctgctcatcc ctatacattg gggatctgtg cggctctctc tttttggcgg
      361 gacaactatt caccttccag ccccgccgac attggaccgt gcaagactgc aactgctcca
      421 tctatacagg ccacgttacc ggccacagga tggcttggga catgatgatg aattggtcac
      481 ccacaaccac tctggtccta tctagcatct tgagggtacc tgagatctgt gcgagtgtga
      541 tactcggtgg ccactggggg atactattgg ccgtcgccca ctttggtatg gctggcaact
      601 ggctaaaagt tctggctgtt ctgttcttat ttgcaggggt tgaagcaacc accaccaccg
      661 cccacgaagc ggcccgcgct actgccggct tcgttgggct tttcacctcc ggtgccaggc
      721 agaagctaca actcatcaac accaacggca gctggcatat aaacaggact gctttgaact
      781 gcaatgattc cctccagacg gggttcatag cgtcactctt ttacttcaac aaattcaact
      841 cctcgggttg cccggagcgg atggctgcgt gtaagcccct cgcggatttc cggcaggggt
      901 ggggccaaat aacctacaag gtcaacatct cgggcccctc tgacgaccgt ccctactgct
      961 ggcattacgc tcctaggtca tgtgacgtgg tgccggcccg cacggtgtgc ggccctgttt
     1021 actgcttcac gcccagccct gttgtggtag gaaccactga taggcggggc aaccccacat
     1081 acacctgggg ggagaatgag acggacgtat tcatgctgga aagccttcgg ccccctactg
     1141 gaggatggtt tgggtgcacg tggatgaact ctacgggctt taccaagacc tgtggtgccc
     1201 cgccatgtca gatagtccca ggagattaca acagctctgc caacgagctt ttgtgcccca
     1261 ccgactgctt ccgtaaacat ccggaagcta cataccagcg gtgcggatcg ggaccctggc
     1321 tgacacctag gtgtctggtg gattaccctt acaggctatg gcactacccc tgcactgtca
     1381 actttaccgt gcataaagtc aggatgttcg tgggaggcat tgagcaccgg tttgacgctg
     1441 catgtaattg gaccaggggc gagcggtgcg agctacatga cagagacagg attgaaatga
     1501 gcccgctgct tttctcaact acgcagctcg ccatacttcc ctgctcattt tccaccatgc
     1561 cggccttgtc aactggcctc atccatctgc atcagaacat agtggacgtg cagtacctct
     1621 acggagtatc ctcgagcgtc acctcgtggg tggtgaagtg ggagtacatt gtcctgatgt
     1681 tcctggttct ggcagatgct cggatttgta cgtgtctctg gttaatgctg ctcataacca
     1741 acgttgaagc ataaaagccg aattctgcag atatccagca cagtggcggc cgctcgagtc
     1801 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1861 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1921 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1981 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2041 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agtggcggta atacggttat
     2101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg
     2941 aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
     3001 ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
     3061 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     3121 caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta
     3181 ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga
     3241 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     3301 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
     3361 cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     3421 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     3481 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     3541 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     3601 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt
     3661 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     3721 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     3781 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     3841 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     3901 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     3961 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc
     4021 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4081 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4141 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     4201 tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4261 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4321 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4381 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4441 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4501 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     4561 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
     4621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4921 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5221 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5821 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5941 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6061 aaatattaac gcttacaatt tacgcgcgcg ttgacattga ttattgacta gttattaata
     6121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     6181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     6301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     6481 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     6541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     6601 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     6661 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     6721 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
     6781 gagctcggat ccactagtcc agtgtggtgg aattcggct
//
