LOCUS       sequence-190108-        6819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP6.1.2
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1824..1841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1830..2054
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2186..2774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2266..2285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3103..3150
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3175..3194
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3382..4176)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3382..4176)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3493..3512)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4103..4122
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4211..4568)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4225..4360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4377..4568
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4525..4545
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4628..5419,5420..5488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5251..5270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5489..5593)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5620..6075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5707..5726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5917..5938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6087..6466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6467..6670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6621..6641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6715..6734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6715..6733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgggttgctc tttctctatc ttccttttgg cactactctc gtgcctcaca acgccggctt
       61 cggctcttac ctacggtaac tccagtgggc tgtaccatct tacaaatgat tgccccaact
      121 ccagcatcgt gctggaagcg gatgctatga tcttgcatct gcctggatgc ttgccttgtg
      181 tgagggtcga taatcggtcc acctgctggc atgctgtgtc ccccacccta gccataccaa
      241 atgcttctac gcccgcaacg ggattccgca ggcatgtgga tcttcttgcg ggcgccgcag
      301 tggtctgctc atccctatac attggggatc tgtgcggctc tctctttttg gcgggacaac
      361 tattcacctt ccagccccgc cgacattgga ccgtgcaaga ctgcaactgc tccatctata
      421 caggccacgt taccggccac aggatggctt gggacatgat gatgaattgg tcacccacaa
      481 ccactctggt cctatctagc atcttgaggg tacctgagat ctgtgcgagt gtgatactcg
      541 gtggccactg ggggatacta ttggccgtcg cctactttgg tatggctggc aactggctaa
      601 aagttctggc tgttctgttc ttatttgcag gggttgaagc aaccaccacc accgcccacg
      661 aagcggcccg cgctactgcc ggcttcgttg ggcttttcac ctccggtgcc aggcagaagc
      721 tacaactcat caacaccaac ggcagctggc atataaacag gactgctttg aactgcaatg
      781 attccctcca gacggggttc atagcgtcac tcttttactt caacaaattc aactcctcgg
      841 gttgcccgga gcggatggct gcgtgtaagc ccctcgcgga tttccggcag gggtggggcc
      901 aaataaccta caaggtcaac atctcgggcc cctctgacga ccgtccctac tgctggcatt
      961 acgctcctag gtcatgtgac gtggtgccgg cccgcacggt gtgcggccct gtttactgct
     1021 tcacgcccag ccctgttgtg gtaggaacca ctgataggcg gggcaacccc acatacacct
     1081 ggggggagaa tgagacggac gtattcatgc tggaaagcct tcggccccct actggaggat
     1141 ggtttgggtg cacgtggatg aactctacgg gctttaccaa gacctgtggt gccccgccat
     1201 gtcagatagt cccaggagat tacaacagct ctgccaacga gcttttgtgc cccaccgact
     1261 gcttccgtaa acatccggaa gctacatacc agcggtgcgg atcgggaccc tggctgacac
     1321 ctaggtgtct ggtggattac ccttacaggc tatggcacta cccctgcact gtcaacttta
     1381 ccgtgcataa agtcaggatg ttcgtgggag gcattgagca ccggtttgac gctgcatgta
     1441 attggaccag gggcgagcgg tgcgagctac atgacagaga caggattgaa atgagcccgc
     1501 tgcttttctc aactacgcag ctcgccatac ttccctgctc attttccacc atgccggcct
     1561 tgtcaactgg cctcatccat ctgcatcaga acatagtgga cgtgcagtac ctctacggag
     1621 tatcctcgag cgtcacctcg tgggtggtga agtgggagta cattgtcctg atgttcctgg
     1681 ttctggcaga tgctcggatt tgtacgtgtc tctggttaat gctgctcata accaacgttg
     1741 aagcataaaa gccgaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     1801 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1861 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1921 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1981 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     2041 ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg ttatccacag
     2101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta
     2941 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
     3001 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
     3061 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
     3121 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     3181 ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc
     3241 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     3301 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     3361 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3421 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3481 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3541 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3601 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     3661 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     3721 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     3781 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     3841 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     3901 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     3961 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     4021 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     4081 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     4141 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     4201 ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
     4261 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     4321 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     4381 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     4441 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4501 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
     4561 cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag taaacttggt
     4621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5641 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5701 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5761 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5821 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5881 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5941 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6001 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6061 taacgcttac aatttacgcg cgcgttgaca ttgattattg actagttatt aatagtaatc
     6121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6661 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
     6721 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc
     6781 ggatccacta gtccagtgtg gtggaattcg gcttcacca
//
