LOCUS       sequence-190109-        3091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector NKX2-5 _HD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Nkx2-5 delta HD-G8
FEATURES             Location/Qualifiers
     source          1..3091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(343..442)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(554..573)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             565..1374
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(632..651)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1464..2052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1953..1972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2217..2303
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2395..2422
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     2430..2453
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    2472..2571
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggatcgcggt gcccgtgctg gtgcgcgacg ggaagccctg cctgggggac cccgcggcct
       61 acgctcccgc ctacggcgtg ggtctcaatg cctatggcta caacgcctac ccctacccca
      121 gctacggcgg cgcggcctgc agtcccggct acagctgcgc cgcctacccc gctgcgcccc
      181 ccgccgcgca gccccccgcc gcctccgcca acagcaactt cgtgaacttt ggcgtcgggg
      241 acttgaacac cgtgcagagt cccgggatgc cgcagggcaa ttcgggcgtc tccacgctgc
      301 acggcatccg agcctggtcg cggccgcact cgagatatct agacccagct ttcttgtaca
      361 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      421 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
      481 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      541 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
      601 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      661 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
      721 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      781 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      841 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
      901 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      961 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1021 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1081 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1141 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1201 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1261 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1321 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     1381 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc
     1441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct
     2161 agcatggatc tcggggacgt ctaactacta agcgagagta gggaactgcc aggcatcaaa
     2221 taaaacgaaa ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2281 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc
     2341 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaact aagcagaagg
     2401 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctgttagt tagttactta
     2461 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     2521 ttgataagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tggcgccgga
     2581 accaattcag tcgactggat ccgaccatgt tccccagccc tgcgctcaca cccacgcctt
     2641 tctcagtcaa agacatcctg aacctggagc agcagcagcg tagcctggcg tctggggacc
     2701 tgtctgcgcg cctcgaggcc accctggccc ctgcctcctg catgctggcc gccttcaagc
     2761 ccgaggccta ctctggcccc gaggcggcag cgtccggcct ggcagagctg cgcgcggaga
     2821 tgggccccgc gccttcgccc cccaagtgct ctcctgcttt cccagccgcc cccacatttt
     2881 acccgggagc ctacggtgac cctgacccag ccaaagaccc tcgggcggat aaaaaagagc
     2941 tgtgcgcgct gcagaaggca gtggagctgg acaaagccga gacggatggc gccgagagac
     3001 cacgcgcacg gcggcgacgg aagccacgcg gcggcggcgg cggcggcggc ggcactctgg
     3061 agcttctggg gccgccgccg ccgcccgcgc g
//
