LOCUS       sequence-190110-        3093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector NKX2-5 _HD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label: pENTR-2B-Nkx2-5 delta HD-G8
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(965..1064)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1176..1195)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1187..1996
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1254..1273)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2086..2674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2575..2594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2839..2925
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3017..3044
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3052..3075
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cgaccatgtt ccccagccct gcgctcacac ccacgccttt ctcagtcaaa
      181 gacatcctga acctggagca gcagcagcgt agcctggcgt ctggggacct gtctgcgcgc
      241 ctcgaggcca ccctggcccc tgcctcctgc atgctggccg ccttcaagcc cgaggcctac
      301 tctggccccg aggcggcagc gtccggcctg gcagagctgc gcgcggagat gggccccgcg
      361 ccttcgcccc ccaagtgctc tcctgctttc ccagccgccc ccacatttta cccgggagcc
      421 tacggtgacc ctgacccagc caaagaccct cgggcggata aaaaagagct gtgcgcgctg
      481 cagaaggcag tggagctgga caaagccgag acggatggcg ccgagagacc acgcgcacgg
      541 cggcgacgga agccacgcgg cggcggcggc ggcggcggcg gcactctgga gcttctgggg
      601 ccgccgccgc cgcccgcgcg caggatcgcg gtgcccgtgc tggtgcgcga cgggaagccc
      661 tgcctggggg accccgcggc ctacgctccc gcctacggcg tgggtctcaa tgcctatggc
      721 tacaacgcct acccctaccc cagctacggc ggcgcggcct gcagtcccgg ctacagctgc
      781 gccgcctacc ccgctgcgcc ccccgccgcg cagccccccg ccgcctccgc caacagcaac
      841 ttcgtgaact ttggcgtcgg ggacttgaac accgtgcaga gtcccgggat gccgcagggc
      901 aattcgggcg tctccacgct gcacggcatc cgagcctggt cgcggccgca ctcgagatat
      961 ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1021 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct
     1081 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1141 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1201 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     1261 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     1321 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     1381 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     1441 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     1501 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1561 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     1621 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     1681 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     1741 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1801 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1861 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     1921 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     1981 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc
     2041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2761 tgtggataac cgtattaccg ctagcatgga tctcggggac gtctaactac taagcgagag
     2821 tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt
     2881 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     2941 ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     3001 aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     3061 cttcctgtta gttagttact taagctcggg ccc
//
