LOCUS       sequence-190119-        4580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing Pseudomonas aeruginosa FabH protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, 
            Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, 
            Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, 
            Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden 
            DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, 
            Naismith JH
  TITLE     The Scottish Structural Proteomics Facility: targets, methods and 
            outputs.
  JOURNAL   J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 
            10.1007/s10969-010-9090-y. Epub 2010 Apr 24.
  PUBMED    20419351
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10969-010-9090-y"; journalName: "J Struct Funct Genomics";
            date: "2010-06"; volume: "11"; issue: "2"; pages: "167-80"
COMMENT     Sequence Label: pEAHISPAFabH
FEATURES             Location/Qualifiers
     source          1..4580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(827..845)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      841..888
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      925..1380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1012..1031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1222..1243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1406..1510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1511..1579,1580..2371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1729..1748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2542..3130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3031..3050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3284..3301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3316..3455
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3381..3400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3541..3563
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3557..3748)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4257..4276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4257..4275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4307..4329
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4349..4366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4388..4408
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgcgcctggg gttggtcggc gcgctcgcgt tcgacctgtc cgccgcctgc accggcttcg
       61 tctacggcct ggccagcgtc ggctcgctga tcagcgccgg gctggcggac agcgcgctgc
      121 tggtcggggt ggacactttc agccataccc tcgaccccgc cgatcgctcg acccgcgcac
      181 tgttcggcga cggcgccgga gcggtggtgc tgcgtgccgg cgatgccgag gaagaaggcg
      241 cgctgctggc cttcgacctc ggcagcgacg gccaccagtt cgacctgctg atgacccccg
      301 ccgtcagtcg cgccgaacgc agttccggac aggcctccaa ctacttccgg atggacggca
      361 aggcagtgtt cggccaggcg gtgacgcaga tgagcgactc ggtgcggcgg gtgctcgacc
      421 gggtcggctg gcaagcttcg gacctccatc acctggtccc gcaccaggcc aacacacgca
      481 ttctcgcggc ggtcgccgac cagctcgacc ttcccgtcga gcgagtggtg agcaacatcg
      541 ccgaggtggg caataccgtc gccgcctcga ttcccctggc cctggcccac ggcctgcgcc
      601 aaggcatcct gcgcgacggc ggcaacatgg tcctcaccgg tttcggtgcc ggactgacct
      661 ggggttcggt cgccctgcgc tggccgaaga tcgttccgac aatggactga ggatccgaat
      721 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      781 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      841 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      901 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1081 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1141 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1201 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1261 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1321 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2041 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3301 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3361 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3421 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3481 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3541 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     3601 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     3661 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     3721 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     3781 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     3841 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     3901 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     3961 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     4021 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     4081 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     4141 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     4201 tcatgcgcac ccgtggccag gacccaacgc tgcccgagat ctcgatcccg cgaaattaat
     4261 acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta
     4321 agaaggagat atacatatgt cgtactacca tcaccatcac catcacgatt acgacatccc
     4381 aacgaccgaa aacctgtatt ttcagggcgc catggcgcgc gccgccgtgg tctgcggcct
     4441 gggcagctac ctgcccgagg ccgtgctcag caacgacatg ctcgccgccg agctggacac
     4501 ttccgacgcc tggatcagca gccgcaccgg cgtgcgccag cggcatatcg ccggcgacct
     4561 cggcagcggc gacctggccc
//
