LOCUS       sequence-190125-        7545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, 
            Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, 
            Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, 
            Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden 
            DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, 
            Naismith JH
  TITLE     The Scottish Structural Proteomics Facility: targets, methods and 
            outputs.
  JOURNAL   J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 
            10.1007/s10969-010-9090-y. Epub 2010 Apr 24.
  PUBMED    20419351
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10969-010-9090-y"; journalName: "J Struct Funct Genomics";
            date: "2010-06"; volume: "11"; issue: "2"; pages: "167-80"
COMMENT     Sequence Label: pDEHISSIRV55
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(492..519)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(611..697)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     746..768
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     760..777
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     761..777
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    793..892
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             922..939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             961..981
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1151..1250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1409..1428)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1420..2229
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1487..1506)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2376..2964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3150..3289
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3215..3234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3375..3397
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3391..3582)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5044..5063
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5147..5165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5193..5217
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5247..5264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5286..5306
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(5476..5500)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5553..5571)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5567..5614
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5980..5985,5986..6002,6003..6008))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6035..6053)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6121..6225
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6226..6294,6295..7086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6444..6463)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
       61 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      421 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      481 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      541 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      601 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      661 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      721 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
      781 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      841 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcagg cttcgaagga
      901 gatatacata tgtcgtacta ccatcaccat caccatcacg attacgatat cccaacgacc
      961 gaaaacctgt attttcaggg catgaaaaag gaaatacaag ttcaaggcgt aagatattat
     1021 gtagagtcag aagatgactt agttagcgtg gctcacgagt tggctaagat gggttacaca
     1081 gttcaacaga tagcaaacgc tttaggcgtt tcagagagaa aggtacgcag atatttggag
     1141 agttgttagg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1201 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1381 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1441 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1501 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1561 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1621 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1681 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1741 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1801 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1861 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1921 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1981 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2041 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2101 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2161 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2221 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2281 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3121 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     3181 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3241 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     3301 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3361 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3421 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     3481 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     3541 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     3601 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     3661 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     3721 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     3781 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     3841 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     3901 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     3961 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     4021 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
     4081 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     4141 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     4201 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     4261 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     4321 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
     4381 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     4441 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     4501 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     4561 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     4621 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     4681 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     4741 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     4801 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgatcgac gctctccctt
     4861 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     4921 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     4981 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     5041 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     5101 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     5161 atagggagac cacaacggtt tccctctaga tcacaagttt gtacaaaaaa gcaggcttcg
     5221 aaggagatat acatatgtcg tactaccatc accatcacca tcacgattac gatatcccaa
     5281 cgaccgaaaa cctgtatttt cagggcatga aaaaggaaat acaagttcaa ggcgtaagat
     5341 attatgtaga gtcagaagat gacttagtta gcgtggctca cgagttggct aagatgggtt
     5401 acacagttca acagatagca aacgctttag gcgtttcaga gagaaaggta cgcagatatt
     5461 tggagagttg ttaggaccca gctttcttgt acaaagtggt gatgatccgg ctgctaacaa
     5521 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     5581 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     5641 tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag
     5701 cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata
     5761 gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg
     5821 aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     5881 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     5941 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
     6001 gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     6061 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6121 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6181 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6241 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6301 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6361 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6421 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6481 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6541 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6601 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6661 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6721 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6781 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6841 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6901 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6961 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7021 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7081 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7141 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7201 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7261 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7321 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7381 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7441 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7501 agtggcgata agtcgtgtct taccgggttg gactcaagac gatag
//
