LOCUS       sequence-190131-        5073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, 
            Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, 
            Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, 
            Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden 
            DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, 
            Naismith JH
  TITLE     The Scottish Structural Proteomics Facility: targets, methods and 
            outputs.
  JOURNAL   J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 
            10.1007/s10969-010-9090-y. Epub 2010 Apr 24.
  PUBMED    20419351
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10969-010-9090-y"; journalName: "J Struct Funct Genomics";
            date: "2010-06"; volume: "11"; issue: "2"; pages: "167-80"
COMMENT     Sequence Label: pDEHISSIRV119
FEATURES             Location/Qualifiers
     source          1..5073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..160
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(522..546)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(599..617)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      613..660
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1026..1031,1032..1048,1049..1054))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1081..1099)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1167..1271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1272..1340,1341..2132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1490..1509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2303..2891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2792..2811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3045..3062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3077..3216
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3142..3161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3302..3324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3318..3509)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4971..4990
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagatcacaa gtttgtacaa
       61 aaaagcaggc ttcgaaggag atatacatat gtcgtactac catcaccatc accatcacga
      121 ttacgatatc ccaacgaccg aaaacctgta ttttcagggc atggatttga aaaaagtttt
      181 aaattttcat ttttcttata tttacactta tttcattact attacaacta attataagta
      241 tggagatact gaaaaaatat ttagaaaatt tcgtagttat atttataatc atgataaaaa
      301 ttcacatgta ttttctataa aagaaactac aaaaaattca aatggtctac attatcatat
      361 tttagttttc acgaataaaa aattagatta ttctagagtt cacaaacata tgccttcaca
      421 ttctgacatt agaattgaat tagttccaaa gtctattagc gatataaaaa atgtatataa
      481 gtatatgcta aagacaaaaa aagatataaa aatgtcatag gacccagctt tcttgtacaa
      541 agtggtgatg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      601 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      661 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta
      721 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
      781 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
      841 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
      901 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
      961 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     1021 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     1081 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1261 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1321 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1381 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1441 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1501 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1561 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1621 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1681 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1741 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1801 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1861 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1921 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1981 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2041 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2101 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3001 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3061 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3121 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     3181 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     3241 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3301 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     3361 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     3421 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     3481 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     3541 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     3601 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     3661 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     3721 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     3781 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     3841 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     3901 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     3961 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     4021 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     4081 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     4141 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     4201 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     4261 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
     4321 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     4381 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     4441 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     4501 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     4561 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     4621 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     4681 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     4741 ctgggttgaa ggctctcaag ggcatcggtc gatcgacgct ctcccttatg cgactcctgc
     4801 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     4861 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     4921 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     4981 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     5041 gcgtagagga tcgagatctc gatcccgcga aat
//
