LOCUS       sequence-190144-       11974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31,
            Vika, B3, and Bxb1 recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Weinberg BH, Pham NT, Caraballo LD, Lozanoski T, Engel A, Bhatia S, 
            Wong WW
  TITLE     Large-scale design of robust genetic circuits with multiple inputs 
            and outputs for mammalian cells.
  JOURNAL   Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805.
  PUBMED    28346402
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3805."
COMMENT     Sequence Label: pBW829_pCAG-pExpr-LUT
FEATURES             Location/Qualifiers
     source          1..11974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..26
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     88..107
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    206..239
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(241..274)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    287..320
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     polyA_signal    666..721
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(join(864..1577,1578..1580,1581..1583))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(867..1577,1578..1580,1581..1583))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      1580..1589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1699..1732)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             1815..2609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2789..2923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2969..2978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2975..2977,2978..2980,2981..3694)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2975..2977,2978..2980,2981..3691)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3837..3892
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    4292..4325
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(4327..4360)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4442..5236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5416..5550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      5595..5604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5601..5603,5604..5606,5607..6320)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5601..5603,5604..5606,5607..6317)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    6463..6518
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(6918..6951)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6958..7007
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     polyA_signal    7347..7402
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(join(7545..8258,8259..8261,8262..8264))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7548..8258,8259..8261,8262..8264))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      8261..8270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    8275..8322
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    8577..8632
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8993..9009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8993..9009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9006..9028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9017..9033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9041..9047,9048..9065,9066..9071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9086..9107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      9192..9326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9346..9480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9548..9565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9719..10307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9799..9818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10478..11269,11270..11338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11101..11120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11339..11443)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11474..11853
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
       61 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
      121 ttggcaaaga attgattaat tcgagcgaag gcgcgcccat tactcgcatc cattctcagg
      181 ctgtctcgtc tcgtctcgca cgcgtgaagt tcctattctc tagaaagtat aggaacttcg
      241 ataacttcgt ataggatact ttatacgaag ttataccggt gtgcgggtgc cagggcgtgc
      301 ccttgggctc cccgggcgcg tactccctgc aggtcgaggg atctccataa gagaagaggg
      361 acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa aacatgtaag
      421 gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa aaaaatctaa
      481 cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg tttcatatac
      541 tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc atatgttgcc
      601 aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt
      661 ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat
      721 tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga
      781 aaaagatctc agtggtattt gtgagccagg gcattggcca caccagccac caccttctga
      841 taggcagcct gcacctgagg agtttacttg tacagctcgt ccatgccgag agtgatcccg
      901 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      961 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     1021 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     1081 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     1141 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     1201 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     1261 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     1321 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     1381 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     1441 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     1501 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     1561 agctcctcgc ccttgctcac catggtggcg gcacgccccc aactgagaga actcaaaggt
     1621 taccccagtt ggggcactga attcggtacc gctgggagtt cgtagacgga aacaaacgca
     1681 gaatccaagc gcacgcgtat aacttcgtat aggatacttt atacgaagtt ataccggtaa
     1741 taggtctgag aacgcccatt ctcagacgta ttcggatttg atctgatcaa gagacaggat
     1801 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1861 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     1921 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     1981 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     2041 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     2101 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     2161 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2221 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     2281 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     2341 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     2401 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     2461 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     2521 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     2581 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     2641 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     2701 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     2761 gctggagttc ttcgcccacc ccatcgataa cttgtttatt gcagcttata atggttacaa
     2821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2881 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaaatccg aaataggtct
     2941 gagaacgccc attctcagac gtattgccgc caccatggtg agcaagggcg aggagctgtt
     3001 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3061 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3121 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3181 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3241 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3301 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3361 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3421 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3481 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3541 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     3601 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3661 gatcactctc ggcatggacg agctgtacaa gtaaactcct caggtgcagg ctgcctatca
     3721 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3781 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3841 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3901 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3961 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     4021 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     4081 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     4141 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     4201 tcttctctta tggagatccc tcgacctgca ggaattcggt accgcactga aggtcctcaa
     4261 tcgcactgga aacatcaagg tcggcacgcg tgaagttcct attctctaga aagtatagga
     4321 acttcgataa cttcgtatag catacattat acgaagttat accggtggtt gcttaagaat
     4381 aagtaattct taagcaaccc ggatttgatc tgatcaagag acaggatgag gatcgtttcg
     4441 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4501 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4561 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4621 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4681 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4741 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4801 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4861 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4921 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     4981 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5041 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5101 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5161 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5221 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5281 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5341 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5401 gcccacccca tcgataactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5521 ctcatcaatg tatcttatca tgtctggatc aaatccgagg ttgcttaaga ataagtaatt
     5581 cttaagcaac cgccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5641 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5701 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5761 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     5821 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5881 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5941 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6001 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6061 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6121 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6181 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     6241 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     6301 tggacgagct gtacaagtaa actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     6361 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     6421 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     6481 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     6541 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     6601 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     6661 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     6721 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     6781 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     6841 gatccctcga cctgcaggaa ttcggtaccc tgacctcctg ccagcaatag taagacaaca
     6901 cgcaaagtcg cacgcgtata acttcgtata gcatacatta tacgaagtta taccggttcg
     6961 gccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccgggcctg caggtcgagg
     7021 gatctccata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
     7081 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
     7141 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
     7201 gcagggggct gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc
     7261 agcatatggg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     7321 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     7381 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     7441 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
     7501 acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtttactt gtacagctcg
     7561 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     7621 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     7681 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     7741 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     7801 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     7861 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     7921 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     7981 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     8041 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     8101 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     8161 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     8221 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc ggcgggtttg
     8281 taccgtacac cactgagacc gcggtggttg accagacaaa ccacgagaat tcggtaccga
     8341 gccaactccc tttacaacct cactcaagtc cgttagaggc ggccgcccag gatacataga
     8401 ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg ctgcctatca
     8461 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     8521 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     8581 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     8641 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     8701 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     8761 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     8821 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     8881 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     8941 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     9001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     9181 agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     9361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9481 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11461 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
    11521 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
    11581 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
    11641 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
    11701 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
    11761 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
    11821 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
    11881 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
    11941 ttttgtgcag cgatgggggc gggggggggg gggg
//
