LOCUS       sequence-190155-        8167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression of TRAF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8167)
  TITLE     Karin Lab plasmids
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLU-CMV-PL3 Flag-humanTRAF3-WT (1114)
FEATURES             Location/Qualifiers
     source          1..8167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..37
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(75..97)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     197..216
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     410..432
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     424..441
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     425..441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        456..682
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             683..863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    910..1035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1528..1761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1946..1990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2257..2373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2396..2699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2700..2903
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2854..2874
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2900..2924
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3019..3042
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4684..5272
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4737..4757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5155..5166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5359..5592
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5664..5785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5701..5720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5755..5774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5825..5960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5992..6008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5992..6008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6005..6027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6016..6032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6040..6046,6047..6064,6065..6070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6085..6106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6223..6240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6394..6982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6474..6493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7153..7944,7945..8013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7776..7795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8014..8118)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
       61 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
      121 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      181 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
      301 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      361 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      421 cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa tactcttgta
      481 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      541 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      601 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      661 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1381 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1441 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1501 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1561 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1621 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1681 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1741 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1801 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1861 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1921 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1981 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2041 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2101 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2161 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2221 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     2281 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2341 attacaaaaa caaattacaa aattcaaaat tttatcgata agcttgggag ttccgcgtta
     2401 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2461 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2521 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2581 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2641 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2701 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2761 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2821 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2881 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2941 cacgctgttt tgacctccat agaagacacc gactctagaa cctgcagcaa gctttgatca
     3001 gctagcctcg agaccatgga ctacaaggac gacgatgaca aggagtcgag taaaaagatg
     3061 gactctcctg gcgcgctgca gactaacccg ccgctaaagc tgcacactga ccgcagtgct
     3121 gggacgccag tttttgtccc tgaacaagga ggttacaagg aaaagtttgt gaagaccgtg
     3181 gaggacaagt acaagtgtga gaagtgccac ctggtgctgt gcagcccgaa gcagaccgag
     3241 tgtgggcacc gcttctgcga gagctgcatg gcggccctgc tgagctcttc aagtccaaaa
     3301 tgtacagcgt gtcaagagag catcgttaaa gataaggtgt ttaaggataa ttgctgcaag
     3361 agagaaattc tggctcttca gatctattgt cggaatgaaa gcagaggttg tgcagagcag
     3421 ttaatgctgg gacatctgct ggtgcattta aaaaatgatt gccattttga agaacttcca
     3481 tgtgtgcgtc ctgactgcaa agaaaaggtc ttgaggaaag acctgcgaga ccacgtggag
     3541 aaggcgtgta aataccggga agccacatgc agccactgca agagtcaggt tccgatgatc
     3601 gcgctgcaga aacacgaaga caccgactgt ccctgcgtgg tggtgtcctg ccctcacaag
     3661 tgcagcgtcc agactctcct gaggagcgag gggacaaacc agcagatcaa ggcccacgag
     3721 gccagctccg ccgtgcagca cgtcaacctg ctgaaggagt ggagcaactc gctcgaaaag
     3781 aaggtttcct tgttgcagaa tgaaagtgta gaaaaaaaca agagcataca aagtttgcac
     3841 aatcagatat gtagctttga aattgaaatt gagagacaaa aggaaatgct tcgaaataat
     3901 gaatccaaaa tccttcattt acagcgagtg atagacagcc aagcagagaa actgaaggag
     3961 cttgacaagg agatccggcc cttccggcag aactgggagg aagcagacag catgaagagc
     4021 agcgtggagt ccctccagaa ccgcgtgacc gagctggaga gcgtggacaa gagcgcgggg
     4081 caagtggctc ggaacacagg cctgctggag tcccagctga gccggcatga ccagatgctg
     4141 agtgtgcacg acatccgcct agccgacatg gacctgcgct tccaggtcct ggagaccgcc
     4201 agctacaatg gagtgctcat ctggaagatt cgcgactaca agcggcggaa gcaggaggcc
     4261 gtcatgggga agaccctgtc cctttacagc cagcctttct acactggtta ctttggctat
     4321 aagatgtgtg ccagggtcta cctgaacggg gacgggatgg ggaaggggac gcacttgtcg
     4381 ctgttttttg tcatcatgcg tggagaatat gatgccctgc ttccttggcc gtttaagcag
     4441 aaagtgacac tcatgctgat ggatcagggg tcctctcgac gtcatttggg agatgcattc
     4501 aagcccgacc ccaacagcag cagcttcaag aagcccactg gagagatgaa tatcgcctct
     4561 ggctgcccag tctttgtggc ccaaactgtt ctagaaaatg ggacatatat taaagatgat
     4621 acaattttta ttaaagtcat agtggatact tcggatctgc ccgatccctg aggatccgtc
     4681 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4741 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4801 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4861 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4921 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4981 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5041 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5101 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5161 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5221 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     5281 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5401 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5461 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5521 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5581 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5641 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5761 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5881 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5941 aggaggcttt tttggaggcc taggcttttg cgggcccaaa ttcgtaatca tgtcatagct
     6001 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6061 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6121 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6181 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7141 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7201 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7261 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7321 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7381 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7441 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7501 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7561 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7621 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7681 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7741 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7801 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7861 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7921 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7981 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8041 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8101 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8161 tattatc
//
