LOCUS       sequence-190175-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and 
            Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the 
            control of CUP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pJT3
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        558..792
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(2945..2965)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2945..2963)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2984..3000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2984..3000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2997..3019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3008..3024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3032..3038,3039..3056,3057..3062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3077..3098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3215..3232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3386..3974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3466..3485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4145..4936,4937..5005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4768..4787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5006..5110)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5137..6479
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6100..6147)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6520..6538
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6576..6598)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6698..6717
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7211..8305)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(8306..8713)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(9014..9469)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9151..9172)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9363..9382
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9595..9617
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9609..9626
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9610..9626
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9636..9655
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9636..9654
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(11112..11185,1..161))
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
ORIGIN
        1 ttctctttta tatgattctg acaatccata ttgcgttggt agtctttttt gctggaacgg
       61 ttcagcggaa aagacgcatc gctctttttg cttctagaag aaatgccagc aaaagaatct
      121 cttgacagtg actgacagca aaaatgtctt tttctaacta gtaacaaggc taagatatca
      181 gcctgaaata aagggtggtg aagtaataat taaatcatcc gtataaacct atacacatat
      241 atgaggaaaa ataatacaaa agtgttttaa atacagatac atacatgaac atatgcacgt
      301 atagcgccca aatgtcggta atgggatcgg cttactcgag gatagataag cttaaggaat
      361 tgtcgactaa gtcgtaaggc atgctaagcc gatcccatta ccgacatttg ggcgctatac
      421 gtgcatatgt tcatgtatgt atctgtattt aaaacacttt tgtattattt ttcctcatat
      481 atgtgtatag gtttatacgg atgatttaat tattacttca ccacccttta tttcaggctg
      541 atatcttagc cttgttacta gttagaaaaa gacatttttg ctgtcagtca ctgtcaagag
      601 attcttttgc tggcatttct tctagaagca aaaagagcga tgcgtctttt ccgctgaacc
      661 gttccagcaa aaaagactac caacgcaata tggattgtca gaatcatata aaagagaagc
      721 aaataactcc ttgtcttgta tcaattgcat tataatatct tcttgttagt gcaatatcat
      781 atagaagtca tcgaaataga tattaagaaa aacaaactgt acaatcaatc aatcaatcat
      841 cacataaagg atccaaaatg gctaccgcag caggtcctat cgcaactaac aactccccac
      901 aaaactccaa cgcttacaga actccaatcg ctccttccgt accaattact cataaatggt
      961 ctatagctga agatttgaca tgtatttcca atcctagtaa gcacaataac cctcaaactg
     1021 gttacagatc attttctgac gaattatacg ttaagtacga agaaaagttg gaagatgtta
     1081 gaaaagcatt aagagaagtt gaagaaaacc ctttggaagg tttagttatg atagacgctt
     1141 tgcaaagatt gggtatcgat taccatttca gaggtgaaat tggtgcattc ttgcaaaagc
     1201 aacaaatcat atcttcaact ccagatggtt accctgaaca tggtttgtac gaagtttcaa
     1261 cattgtttag attcttaaga caagaaggtc acaatgttac cgctgacgtc tttaataact
     1321 tcaaggataa ggaaggtaga ttcagatcag aattgtcaac agatattaga ggtttgatgt
     1381 ccttatacga agcaagtcaa ttgagaatag aaggtgaaga catcttagat caagctgctg
     1441 atttctccag tcaattgtta ggtagatgga caaaagatcc taatcatcac gaagccagat
     1501 tggtttctaa cactttaaca catccatacc acaagtcatt ggctaccttt atgggtcaaa
     1561 aattgtccta catgaactgc aagggtccaa actgggacgg tgtcgataat ttgcaagaat
     1621 tagctaagat ggatttgact atcgtacaaa gtatccatca aaaggaagta ttccaagttt
     1681 ctcaatggtg gaaggataca ggtttagcca atgaattgaa attggctaga aaccaaccat
     1741 tgaagtggta tatgtggcct atggccgctt taaccgatcc aagattcagt gaagaaagag
     1801 ttgaattaac taagcctatt tcttttatat acatcataga tgacatcttc gacgtctatg
     1861 gtaccattga agaattgacc ttgtttactg atgcagttaa tagatgggaa ttgtctgccg
     1921 tcgaacaatt accagactac atgaaagtat gtttcaaggc tttgtacgat gttaccaacg
     1981 aaatcgcata caaaatctat aaaaagcatg gtcaaaaccc tattgattcc ttgcaaaaga
     2041 cttgggctag tttgtgcaat gcatttttag ttgaagcaaa gtggttcgcc tctggtcact
     2101 tgccaaatgc agaagaatac ttaaagaacg gtatcatctc ttcaggtgtt catgttgtct
     2161 tggcccacat gtttttcttg ttaggtgacg gtattacaca agaatcagtt gatttggtag
     2221 atgactatcc aggtatttcc acaagtatcg caaccatttt gagattatct gatgacttgg
     2281 gttcagccaa agatgaagac caagatggtt atgatggttc ttacatcgaa tgttacatga
     2341 aggaacataa gggttccagt gtcgattcag ccagagaaga agtaataaga atgatctccg
     2401 aagcatggaa atgtttgaat aaggaatgct tatcaccaaa ccctttttct gaatcattca
     2461 gaataggttc cttgaatatg gctagaatga tccctatgat gtactcttac gatgacaacc
     2521 ataacttgcc aattttagaa gaacacatga aggcaatgat ctataacaca tctttataat
     2581 agtgaccatg gcggattgag agcaaatcgt taagttcagg tcaagtaaaa attgatttcg
     2641 aaaactaatt tctcttatac aatcctttga ttggaccgtc atcctttcga atataagatt
     2701 ttgttaagaa tattttagac agagatctac tttatattta atatctagat attacataat
     2761 ttcctctcta ataaaatatc attaataaaa taaaaatgaa gcgatttgat tttgtgttgt
     2821 caacttagtt tgccgctatg cctcttgggt aatgctatta ttgaatcgaa gggctttatt
     2881 atattaccct ttagcttatt ctgaggtttc tgtggcgtcc gcggtggagc tccagctttt
     2941 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     3001 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3061 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4261 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4981 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5041 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5101 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     5161 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     5221 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     5281 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     5341 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     5401 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     5461 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     5521 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     5581 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     5641 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     5701 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     5761 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     5821 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     5881 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     5941 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     6001 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     6061 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     6121 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
     6181 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
     6241 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
     6301 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
     6361 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
     6421 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
     6481 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     6541 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     6601 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6661 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     6721 agagtgcacc atatcgacta cgtcgttaag gccgtttctg acagagtaaa attcttgagg
     6781 gaactttcac cattatggga aatggttcaa gaaggtattg acttaaactc catcaaatgg
     6841 tcaggtcatt gagtgttttt tatttgttgt attttttttt ttttagagaa aatcctccaa
     6901 tatataaatt aggaatcata gtttcatgat tttctgttac acctaacttt ttgtgtggtg
     6961 ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc acgttgagcc
     7021 attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc ttcttgataa
     7081 atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt
     7141 tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa
     7201 gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg
     7261 tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa
     7321 ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg
     7381 cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct ggagcagaac
     7441 cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg
     7501 cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac
     7561 caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca
     7621 tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg
     7681 tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg
     7741 accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc
     7801 tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt
     7861 cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac
     7921 ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac
     7981 atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag
     8041 gtctaacact accggtaccc catttaggac cacccacagc acctaacaaa acggcatcag
     8101 ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca tcgatagcag
     8161 caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag
     8221 ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa
     8281 cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata tatatatata
     8341 ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa ccacctattg
     8401 gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat gcaagcattt
     8461 agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc acctttcctt
     8521 tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa
     8581 aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg
     8641 ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac gatacctgag
     8701 tattcccaca gttaactgcg gtcaagatat ttcttgaatc aggcgcctta gaccgctcgg
     8761 ccaaacaacc aattacttgt tgagaaatag agtataatta tcctataaat ataacgtttt
     8821 tgaacacaca tgaacaagga agtacaggac aattgatttt gaagagaatg tggattttga
     8881 tgtaattgtt gggattccat ttttaataag gcaataatat taggtatgta gatatactag
     8941 aagttctcct cgaccggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     9001 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     9061 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     9121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     9181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     9241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     9301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     9361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     9421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     9481 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     9541 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     9601 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
     9661 tgggtaccgg gcccattgta gcaaagattg taaggaaata gcaaaccaga ggtccaagtc
     9721 aaaacggcaa aaaagaagaa agtaagcaat ataatggacg ggtttacatg tttaaatata
     9781 tgtacatgta tgtagtctta tactttcaaa acataatcgc atgcgggtca tcaaaataaa
     9841 cctccatttt ggacttggga aaaagaaaat ttgatttaaa ctgttgtatg tagctaacaa
     9901 tacataaaac tatttaaata aaataggaat aagaatgctc aattaaaaaa tgatttattt
     9961 tctaacaaaa cttcctgcag gtcactatta tttgcttctc ttgtaaactt tgttcaaaaa
    10021 cgcagttaag acgtcggctt tgaagccacg agactcgtcg acttgggaga tcttggcctt
    10081 caaatccttg gcaacagact cttcatattc gtggtataac tggtcgattt tcaagtcatt
    10141 gaaaatcttt ttgcatttgg cttctgcgac tgagtccttc ttaccgtaat tttcgtctaa
    10201 agtctttctt tgttctgcgg aagcaagttc taatgccttg ttgattaccc aagaacattt
    10261 gttatcttgg atatctgtac cgatcttacc gatctgttct ggggtaccga agcagtctaa
    10321 gtagtcatct tgaatttgga aatattcacc caatggaatc aagacatctc tggcttgttt
    10381 caaatccttt tcatctgtga taccggcaac gtacatagcc aatgcgacag gcaagtagaa
    10441 agaatagtaa gcagtcttga aagtaactat gaaggagtgc ttctttaggg agaacttact
    10501 caagtcgact ttgtcttcag gtgcagtgat taagtccatc aattggccca attcggtttg
    10561 gaaggtgact tcatggaaca attcggtgat atctatgtag tatttttcgt ttctgaagtg
    10621 agatttcaaa agcttgtaga tagcagcctc taacatgaat gcgtcattga tggcaatttc
    10681 cccaacttca ggaaccttgt accaacatgg ttggcctctt ctggtaatgg acttgtccat
    10741 catatcatcg gcgaccaaga agtaagcctg caacaactca atgcaccaac ctagaatagc
    10801 aaccttttcg tattcttctt gccccaattg ttcaacggtc ttgttggaga gaatagcata
    10861 cgtgtccaca acggacaaac ctctatttaa cttaccgcct ggagtgttgt agttcaatga
    10921 gtgggcatac cagtcacatg cttccttagg cataccgtaa gccaaaagcg atgcgttcaa
    10981 ttcctctact aatttaggga aaacgttcaa gaatctctct ctcctaattt ctttttctga
    11041 agccattttg ctagctttat gtgatgattg attgattgat tgtacagttt gtttttctta
    11101 atatctattt cgatgacttc tatatgatat tgcactaaca agaagatatt ataatgcaat
    11161 tgatacaaga caaggagtta tttgc
//
