LOCUS       sequence-190177-       12780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant 
            N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 
            under the control of GAL1/GAL2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Peng B, Nielsen LK, Kampranis SC, Vickers CE
  TITLE     Engineered protein degradation of farnesyl pyrophosphate synthase is
            an effective regulatory mechanism to increase monoterpene production
            in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005.
  PUBMED    29471044
REFERENCE   2  (bases 1 to 12780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005."
COMMENT     Sequence Label: pJT10R
FEATURES             Location/Qualifiers
     source          1..12780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(788..835)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1208..1226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1264..1286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1386..1405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1899..2993)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2994..3401)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3702..4157)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3839..3860)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4051..4070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4283..4305
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4297..4314
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4298..4314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4324..4343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4324..4342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5755..6196)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(5761..5784)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    6079..6196
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(10413..10433)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10413..10431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10452..10468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10452..10468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10465..10487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10476..10492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10500..10506,10507..10524,10525..10530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10545..10566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10683..10700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10854..11442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10934..10953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11613..12404,12405..12473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12236..12255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12474..12578)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(12605..12780,1..1167)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
       61 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
      121 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
      181 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
      241 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
      301 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
      361 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
      421 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
      481 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
      541 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
      601 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
      661 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
      721 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
      781 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
      841 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
      901 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
      961 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
     1021 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
     1081 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
     1141 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac
     1201 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     1261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     1321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     1381 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atcgactacg tcgttaaggc
     1441 cgtttctgac agagtaaaat tcttgaggga actttcacca ttatgggaaa tggttcaaga
     1501 aggtattgac ttaaactcca tcaaatggtc aggtcattga gtgtttttta tttgttgtat
     1561 tttttttttt ttagagaaaa tcctccaata tataaattag gaatcatagt ttcatgattt
     1621 tctgttacac ctaacttttt gtgtggtgcc ctcctccttg tcaatattaa tgttaaagtg
     1681 caattctttt tccttatcac gttgagccat tagtatcaat ttgcttacct gtattccttt
     1741 acatcctcct ttttctcctt cttgataaat gtatgtagat tgcgtatata gtttcgtcta
     1801 ccctatgaac atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata
     1861 aagtttatgt acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact
     1921 tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt
     1981 ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc
     2041 aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc
     2101 ttattctttg gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg
     2161 ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgggata
     2221 acggaggctt catcggagat gatatcacca aacatgttgc tggtgattat aataccattt
     2281 aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg atgttgaacc
     2341 ttcaatgtag ggaattcgtt cttgatggtt tcctccacag tttttctcca taatcttgaa
     2401 gaggccaaaa cattagcttt atccaaggac caaataggca atggtggctc atgttgtagg
     2461 gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta ttgttcacta
     2521 tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat acctcccact
     2581 aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat tggagataag
     2641 tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa ttgaagttct
     2701 ttacggattt ttagtaaacc ttgttcaggt ctaacactac cggtacccca tttaggacca
     2761 cccacagcac ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc ctcatctgga
     2821 agtggaacac ctgtagcatc gatagcagca ccaccaatta aatgattttc gaaatcgaac
     2881 ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc ggctgtgatt
     2941 tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggggcaga cattagaatg
     3001 gtatatcctt gaaatatata tatatatatt gctgaaatgt aaaaggtaag aaaagttaga
     3061 aagtaagacg attgctaacc acctattgga aaaaacaata ggtccttaaa taatattgtc
     3121 aacttcaagt attgtgatgc aagcatttag tcatgaacgc ttctctattc tatatgaaaa
     3181 gccggttccg gcgctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata
     3241 tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc
     3301 gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg ctaagagatt
     3361 cgaactcttg catcttacga tacctgagta ttcccacagt taactgcggt caagatattt
     3421 cttgaatcag gcgccttaga ccgctcggcc aaacaaccaa ttacttgttg agaaatagag
     3481 tataattatc ctataaatat aacgtttttg aacacacatg aacaaggaag tacaggacaa
     3541 ttgattttga agagaatgtg gattttgatg taattgttgg gattccattt ttaataaggc
     3601 aataatatta ggtatgtaga tatactagaa gttctcctcg accggtcgat atgcggtgtg
     3661 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     3721 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3781 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3841 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3901 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3961 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4021 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4081 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4141 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4201 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4261 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     4321 gcgtaatacg actcactata gggcgaattg ggtaccgggc ccattgtagc aaagattgta
     4381 aggaaatagc aaaccagagg tccaagtcaa aacggcaaaa aagaagaaag taagcaatat
     4441 aatggacggg tttacatgtt taaatatatg tacatgtatg tagtcttata ctttcaaaac
     4501 ataatcgcat gcgggtcatc aaaataaacc tccattttgg acttgggaaa aagaaaattt
     4561 gatttaaact gttgtatgta gctaacaata cataaaacta tttaaataaa ataggaataa
     4621 gaatgctcaa ttaaaaaatg atttattttc taacaaaact tcctgcaggt cactattatt
     4681 tgcttctctt gtaaactttg ttcaagaacg cagttaagac atcagctttg aagccacgag
     4741 actcatcgac ctgagaaatt ttggccttca aatccttggc aatagactct tcatattcgt
     4801 ggtatagctg ttcaattttc aagtcattga aaatcttttt gcatttggct tctgcgactg
     4861 agtccttctt accgtaattt tcgtctaaag tctttctttg ttctgcggaa gcaagttcta
     4921 atgccttgtt gattacccaa gaacatttgt tatcttggat atctgtaccg atcttaccga
     4981 tctgttctgg ggtaccgaag cagtctaagt agtcatcttg aatttggaag tattcaccca
     5041 atggaatcaa gacatctctg gcttgtttca aatccttttc atccgtgata ccggcaacgt
     5101 acatggccaa tgcgacaggc aagtagaaag aatagtaagc agtcttgaaa gtaactatga
     5161 aggagtgctt ctttagggag aacttactca agtcgacttt gtcttcaggt gcagtgatta
     5221 agtccatcaa ttggcccaat tcggtttgga aggtgacctc atggaacaat tcggtgatat
     5281 ctatgtagta tttttcgttt ctgaagtgag atttcaaaag cttgtagata gcagcctcta
     5341 acatgaatgc gtcccagatg gcaatttccc caacttcagg aaccttgtac caacatggtt
     5401 ggcctcttct ggtaatggac ttgtccatca tatcatcggc gaccaagaag taagcctgca
     5461 acaactcaat gcaccaacct agaatggcaa ccttttcgta ttcttcttgc cccaattgtt
     5521 caacggtctt gttggagaga atagcatacg tgtccacaac ggacaaacct ctatttagct
     5581 taccgcctgg agtgttgtag ttcaatgagt gggcatacca gtcacatgct tccttaggca
     5641 taccgtaagc caaaagcgat gcgttcaatt cctctactaa tttagggaaa acgttcaaga
     5701 atctctctct cctaatttct ttttctgaag ccattttgct agctatagtt ttttctcctt
     5761 gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatg acatttgaat
     5821 aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagcttttg
     5881 catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa
     5941 taaggctaaa aaactaatcg cattattatc ctatggttgt taatttgatt cgttgatttg
     6001 aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat
     6061 tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc
     6121 gaccggtgaa gaccaggacg cacggaggag agtcttccgt cggagggctg tcgcccgctc
     6181 ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt actgaaagtt
     6241 ccaaagagaa ggttttttta ggctaagata atggggctct ttacatttcc acaacatata
     6301 agtaagatta gatatggata tgtatatggt ggtattgcca tgtaatatga ttattaaact
     6361 tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcaatata aactagtaaa
     6421 gccctatact ttgcttcttt atgtaagaag gttggtattc cagctggtgt cgtcaacatc
     6481 gttccaggtc ctggtagaac tgttggtgct gctttgacca acgacccaag aatcagaaag
     6541 ctggctttta ccggttctac agaagtcggt aagagtgttg ctgtcgactc ttctgaatct
     6601 aacttgaaga aaatcacttt ggaactaggt ggtaagtccg cccatttggt ctttgacgat
     6661 gctaacatta agaagacttt accaaatcta gtaaacggta ttttcaagaa cgctggtcaa
     6721 atttgttcct ctggttctag aatttacgtt caagaaggta tttacgacga actattggct
     6781 gctttcaagg cttacttgga aaccgaaatc aaagttggta atccatttga caaggctaac
     6841 ttccaaggtg ctatcactaa ccgtcaacaa ttcgacacaa ttatgaacta catcgatatc
     6901 ggtaagaaag aaggcgccaa gatcttaact ggtggcgaaa aagttggtga caagggttac
     6961 ttcatcagac caaccgtttt ctacgatgtt aatgaagaca tgagaattgt taaggaagaa
     7021 atttttggac cagttgtcac tgtcgcaaag ttcaagactt tagaagaagg tgtcgaaatg
     7081 gctaacagct ctgaattcgg tctaggttct ggtatcgaaa cagaatcttt gagcacaggt
     7141 ttgaaggtgg ccaagatgtt gaaggccggt accgtctgga tcaacacata caacgatttt
     7201 gactccagag ttccattcgg tggtgttaag caatctggtt acggtagaga aatgggtgaa
     7261 gaagtctacc atgcatacac tgaagtaaaa gctgtcagaa ttaagttgta atgtaccaac
     7321 ctgcatttct ttccgtcata tacacaaaat actttcatat aaacttactt ggtcttacgt
     7381 cataaataaa tatgtataca tataaattaa aaaatttggt tttatatttt tacaaaaaga
     7441 atcgtttact tcatttctcc cttttaagcg atacaatcca tgaaaaaaga gaaaaagaga
     7501 gaacaggctt gtgccttctt taaaacatcc cacacaaaat catattgaat tgaattttac
     7561 atcttaagct agtgtacaac aactgctata tccaaagaaa actaacctcg agtgtactaa
     7621 tccaaggagg tttacggacc agaggaactt tccagattca gatcacagca atataggact
     7681 ggaaaacatc aggtagccgc actcaacttg taactggcaa ctactttgca ttaaactcca
     7741 attaaatgcg gtagaatctt ttcagaaaag gtattcaacg tcaattcgga aagcttcctt
     7801 ccggaatggc ttaagtaggt tgcaatttct ttttctatta gtagctaaaa atgggtcacg
     7861 tgatctatat tcgaaagggg cggttgcctc aggaaggcac cggcggtctt tcgtccgtgc
     7921 ggagatatct gcgccgttca ggggtccatg tgccttggac gatattaagg cagaaggcag
     7981 tatcggggcg gatcactccg aaccgagatt agttaagccc ttcccatctc aagatgggga
     8041 gcaaatggca ttatactcct gctagaaagt taactgtgca catattctta aattatacaa
     8101 tgttctggag agctattgtt taaaaaacaa acatttcgca ggctaaaatg tggagatagg
     8161 attagttttg tagacatata taaacaatca gtaattggat tgaaaatttg gtgttgtgaa
     8221 ttgctcttca ttatgcacct tattcaatta tcatcaagaa tagcaatagt taagtaaaca
     8281 caagattaac ataataaaaa aaataattct ttcataggat ccaaaatggc taccgcagca
     8341 ggtcctatcg caactaacaa ctccccacaa aactccaacg cttacagaac tccaatcgct
     8401 ccttccgtac caattactca taaatggtct atagctgaag atttgacatg tatttccaat
     8461 cctagtaagc acaataaccc tcaaactggt tacagatcat tttctgacga attatacgtt
     8521 aagtacgaag aaaagttgga agatgttaga aaagcattaa gagaagttga agaaaaccct
     8581 ttggaaggtt tagttatgat agacgctttg caaagattgg gtatcgatta ccatttcaga
     8641 ggtgaaattg gtgcattctt gcaaaagcaa caaatcatat cttcaactcc agatggttac
     8701 cctgaacatg gtttgtacga agtttcaaca ttgtttagat tcttaagaca agaaggtcac
     8761 aatgttaccg ctgacgtctt taataacttc aaggataagg aaggtagatt cagatcagaa
     8821 ttgtcaacag atattagagg tttgatgtcc ttatacgaag caagtcaatt gagaatagaa
     8881 ggtgaagaca tcttagatca agctgctgat ttctccagtc aattgttagg tagatggaca
     8941 aaagatccta atcatcacga agccagattg gtttctaaca ctttaacaca tccataccac
     9001 aagtcattgg ctacctttat gggtcaaaaa ttgtcctaca tgaactgcaa gggtccaaac
     9061 tgggacggtg tcgataattt gcaagaatta gctaagatgg atttgactat cgtacaaagt
     9121 atccatcaaa aggaagtatt ccaagtttct caatggtgga aggatacagg tttagccaat
     9181 gaattgaaat tggctagaaa ccaaccattg aagtggtata tgtggcctat ggccgcttta
     9241 accgatccaa gattcagtga agaaagagtt gaattaacta agcctatttc ttttatatac
     9301 atcatagatg acatcttcga cgtctatggt accattgaag aattgacctt gtttactgat
     9361 gcagttaata gatgggaatt gtctgccgtc gaacaattac cagactacat gaaagtatgt
     9421 ttcaaggctt tgtacgatgt taccaacgaa atcgcataca aaatctataa aaagcatggt
     9481 caaaacccta ttgattcctt gcaaaagact tgggctagtt tgtgcaatgc atttttagtt
     9541 gaagcaaagt ggttcgcctc tggtcacttg ccaaatgcag aagaatactt aaagaacggt
     9601 atcatctctt caggtgttca tgttgtcttg gcccacatgt ttttcttgtt aggtgacggt
     9661 attacacaag aatcagttga tttggtagat gactatccag gtatttccac aagtatcgca
     9721 accattttga gattatctga tgacttgggt tcagccaaag atgaagacca agatggttat
     9781 gatggttctt acatcgaatg ttacatgaag gaacataagg gttccagtgt cgattcagcc
     9841 agagaagaag taataagaat gatctccgaa gcatggaaat gtttgaataa ggaatgctta
     9901 tcaccaaacc ctttttctga atcattcaga ataggttcct tgaatatggc tagaatgatc
     9961 cctatgatgt actcttacga tgacaaccat aacttgccaa ttttagaaga acacatgaag
    10021 gcaatgatct ataacacatc tttataatag tgaccatggc ggattgagag caaatcgtta
    10081 agttcaggtc aagtaaaaat tgatttcgaa aactaatttc tcttatacaa tcctttgatt
    10141 ggaccgtcat cctttcgaat ataagatttt gttaagaata ttttagacag agatctactt
    10201 tatatttaat atctagatat tacataattt cctctctaat aaaatatcat taataaaata
    10261 aaaatgaagc gatttgattt tgtgttgtca acttagtttg ccgctatgcc tcttgggtaa
    10321 tgctattatt gaatcgaagg gctttattat attacccttt agcttattct gaggtttctg
    10381 tggcgtccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
    10441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10561 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10621 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12601 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
    12661 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
    12721 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
//
