LOCUS       sequence-190179-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the upper-part mevalonate pathway; overexpressing 
            codon-optimized Enterococcus faecalis mevalonate pathway genes under
            the control of CUP1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pPMVAugw
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        296..530
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(3442..3462)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3442..3460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3481..3497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3481..3497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3494..3516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3505..3521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3529..3535,3536..3553,3554..3559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3574..3595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3712..3729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3883..4471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3963..3982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4642..5433,5434..5502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5265..5284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5503..5607)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5634..6976
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6597..6644)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7017..7035
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7073..7095)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7195..7214
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7361..7548
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             7549..8211
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10107..10341)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
ORIGIN
        1 tgttttaaat acagatacat acatgaacat atgcacgtat agcgcccaaa tgtcggtaat
       61 gggatcggct tactcgassw tagatawgct taaggaattg tcgactaagt cgtaaggcat
      121 gctaagccga tcccattacc gacatttggg cgctatacgt gcatatgttc atgtatgtat
      181 ctgtatttaa aacacttttg tattattttt cctcatatat gtgtataggt ttatacggat
      241 gatttaatta ttacttcacc accctttatt tcaggctgat atcttagcct tgttactagt
      301 tagaaaaaga catttttgct gtcagtcact gtcaagagat tcttttgctg gcatttcttc
      361 tagaagcaaa aagagcgatg cgtcttttcc gctgaaccgt tccagcaaaa aagactacca
      421 acgcaatatg gattgtcaga atcatataaa agagaagcaa ataactcctt gtcttgtatc
      481 aattgcatta taatatcttc ttgttagtgc aatatcatat agaagtcatc gaaatagata
      541 ttaagaaaaa caaactgtac aatcaatcaa tcaatcatca cataaaacta gtaaaatgaa
      601 gactgtagta ataatagacg cattgagaac accaatcggt aaatataaag gtagtttgtc
      661 ccaagtttcc gctgtagact taggtactca tgttactaca caattgttga agagacactc
      721 cacaatcagt gaagaaatcg atcaagtcat attcggtaac gtattacaag ctggtaacgg
      781 tcaaaaccca gctagacaaa tagcaatcaa ttctggtttg tcacatgaaa ttcctgctat
      841 gacagtaaac gaagtttgtg gttcaggcat gaaagccgta attttggcta agcaattgat
      901 ccaattgggt gaagccgaag ttttgatcgc tggtggtata gaaaacatga gtcaagctcc
      961 aaaattacaa agattcaatt acgaaactga atcttacgat gcacctttct cttcaatgat
     1021 gtatgatggt ttaactgacg cattttctgg tcaagccatg ggtttgacag cagaaaatgt
     1081 agccgaaaag taccatgtta ccagagaaga acaagatcaa ttttccgttc acagtcaatt
     1141 gaaagctgca caagcacaag ccgaaggtat tttcgctgac gaaatagcac cattagaagt
     1201 ttctggtaca ttggtcgaaa aggatgaagg tataagacct aactccagtg ttgaaaaatt
     1261 gggtactttg aagacagttt tcaaggaaga cggtacagtc accgccggta atgcttctac
     1321 cattaacgat ggtgctagtg cattgataat cgcttctcaa gaatatgccg aagctcatgg
     1381 tttgccatac ttagctatca ttagagatag tgtagaagtt ggtattgacc cagcatatat
     1441 gggtatttct cctataaaag ccatccaaaa gttgttggct agaaaccaat tgaccactga
     1501 agaaatcgat ttgtacgaaa ttaacgaagc atttgccgct acttcaatcg ttgtccaaag
     1561 agaattggca ttgcctgaag aaaaggttaa catctatggt ggtggtatct ccttaggtca
     1621 cgcaattggt gccaccggtg ctagattgtt gacttccttg agttaccaat tgaaccaaaa
     1681 ggaaaagaaa tacggtgttg cttctttgtg cattggtggt ggtttgggtt tagcaatgtt
     1741 gttagaaaga ccacaacaaa agaaaaattc aagattctac caaatgtccc ctgaagaaag
     1801 attggcatca ttgttgaacg aaggtcaaat ttccgccgat actaagaaag aatttgaaaa
     1861 caccgcattg tcttcacaaa tcgccaacca tatgatcgaa aaccaaatct ctgaaacaga
     1921 agttccaatg ggtgtcggtt tgcacttaac tgtagatgaa acagactatt tggttccaat
     1981 ggctaccgaa gaacctagtg ttatcgcagc cttgtctaat ggtgcaaaaa ttgcccaagg
     2041 ttttaagact gttaaccaac aaagattgat gagaggtcaa attgtattct acgatgttgc
     2101 agacgccgaa tcattgatcg atgaattaca agttagagaa actgaaatct ttcaacaagc
     2161 tgaattatct tacccatcaa tcgttaagag aggtggtggt ttgagagatt tgcaatacag
     2221 agcttttgac gaatccttcg tcagtgtaga tttcttggtt gatgtcaaag acgctatggg
     2281 tgcaaatatt gtcaacgcta tgttagaagg tgtagcagaa ttgtttagag aatggttcgc
     2341 tgaacaaaag attttgtttt ctatcttgtc aaactatgct acagaatctg tagttaccat
     2401 gaaaactgca attccagttt ccagattatc taagggttct aacggtagag aaatcgctga
     2461 aaagattgtc ttagcctcaa gatatgcttc cttggaccct tacagagcag ttactcataa
     2521 taagggtata atgaacggta tcgaagcagt cgtattggcc accggtaatg atactagagc
     2581 cgtttctgct tcatgtcacg ctttcgcagt caaggaaggt agatatcaag gtttgacatc
     2641 atggacctta gatggtgaac aattgattgg tgaaatatcc gttccattgg ccttagctac
     2701 tgtcggtggt gctacaaaag tattacctaa gagtcaagct gctgctgatt tgttagctgt
     2761 aacagacgca aaagaattgt caagagttgt cgctgctgtt ggtttggccc aaaatttggc
     2821 cgctttaaga gctttggtct cagaaggtat tcaaaagggt catatggcat tacaagccag
     2881 atccttggct atgacagttg gtgcaaccgg taaagaagtc gaagcagtag cccaacaatt
     2941 gaagagacaa aagactatga accaagacag agctttagca attttgaacg atttgagaaa
     3001 acaataatga tagtattgtt cgttttttat catagttttt caaatcttca tatgaatact
     3061 aggtagaaat ttggcttatc agtacgaatg aattacagtt tgactgattt gtacacgaga
     3121 attgacgtat aacttctttt gaaagtcttt gaaattacat ggtagtacat acatatatat
     3181 atatatatat atatgttata ttattgttaa ttaaatatta ttatgcgtat tttccttttc
     3241 tttattagta tagtattaat gacagtaata ataataataa tagtaacaat atctcttttt
     3301 tttttcagtg agcttttatt tttttttcat tgctcttctt ttggcctctt ttgttttttt
     3361 cttgatttcc tccagtttca tctgtttttt ctttggatca gatacaaaat ctggtttgaa
     3421 cgcgagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     3481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag
     5641 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     5701 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     5761 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca
     5821 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     5881 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     5941 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     6001 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     6061 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     6121 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     6181 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     6241 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     6301 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     6361 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     6421 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     6481 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg
     6541 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag
     6601 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa
     6661 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac
     6721 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt
     6781 atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct
     6841 cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta
     6901 gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt
     6961 cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc tataaaaata
     7021 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     7081 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7141 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     7201 cagagcagat tgtactgaga gtgcaccata aattcccgtt ttaagagctt ggtgagcgct
     7261 aggagtcact gccaggtatc gtttgaacac ggcattagtc agggaagtca taacacagtc
     7321 ctttcccgca attttctttt tctattactc ttggcctcct ctagtacact ctatattttt
     7381 ttatgcctcg gtaatgattt tcattttttt ttttccacct agcggatgac tctttttttt
     7441 tcttagcgat tggcattatc acataatgaa ttatacatta tataaagtaa tgtgatttct
     7501 tcgaagaata tactaaaaaa tgagcaggca agataaacga aggcaaagat gacagagcag
     7561 aaagccctag taaagcgtat tacaaatgaa accaagattc agattgcgat ctctttaaag
     7621 ggtggtcccc tagcgataga gcactcgatc ttcccagaaa aagaggcaga agcagtagca
     7681 gaacaggcca cacaatcgca agtgattaac gtccacacag gtatagggtt tctggaccat
     7741 atgatacatg ctctggccaa gcattccggc tggtcgctaa tcgttgagtg cattggtgac
     7801 ttacacatag acgaccatca caccactgaa gactgcggga ttgctctcgg tcaagctttt
     7861 aaagaggccc taggggccgt gcgtggagta aaaaggtttg gatcaggatt tgcgcctttg
     7921 gatgaggcac tttccagagc ggtggtagat ctttcgaaca ggccgtacgc agttgtcgaa
     7981 cttggtttgc aaagggagaa agtaggagat ctctcttgcg agatgatccc gcattttctt
     8041 gaaagctttg cagaggctag cagaattacc ctccacgttg attgtctgcg aggcaagaat
     8101 gatcatcacc gtagtgagag tgcgttcaag gctcttgcgg ttgccataag agaagccacc
     8161 tcgcccaatg gtaccaacga tgttccctcc accaaaggtg ttcttatgta gtgacaccga
     8221 ttatttaaag ctgcagcata cgatatatat acatgtgtat atatgtatac ctatgaatgt
     8281 cagtaagtat gtatacgaac agtatgatac tgaagatgac aaggtaatgc atcattctat
     8341 acgtgtcatt ctgaacgagg cgcgctttcc ttttttcttt ttgctttttc tttttttttc
     8401 tcttgaactc gacggatcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8461 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     8521 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa ggatccaggt
     8581 acctactgaa attcatgaca caaataacga catattttgt taaagatttg tcggaggtag
     8641 aacttcatgg gcacatgcat tgtattgata aaaaaatgct ggacaagtcg aaattcatgt
     8701 atttatacga agagaaaagc aacgaggaag tgaaagcagc ttctcctcaa aaggagaaga
     8761 tagtagataa tatcattggc ctattatcaa atgatgaaga acattaacat gaattggaaa
     8821 aagaaaaaat tgcatgtaaa tatatacata cgtagaattt tatacttagg ctacagatca
     8881 aagctatcat taatttctgt atgatctaac tgtgttattg atagcactga tggagtactt
     8941 caattcatct tctaaagttt gatcgatgtc agtatccaag gtttcagcaa acatggcttc
     9001 gtattctgca atagataatt cggttctgtt atctaacaaa gccaagtgtg tttccttttg
     9061 caaatggttt tggtaaccag cgaccaattc accagtgaaa aattcggcaa ctgcacctga
     9121 accataagaa aacaaaccga tttggttacc tgcggtcaat gtagtagcgt tttctaacaa
     9181 acttatcaaa cctaagtaca aggaacctgt atacaaatta ccaactcttc tactgtatat
     9241 gatggattct tcgtatctgg ctaatattct ttcttgttct gcttcggttt gatctgaaat
     9301 ctttgctaac aaagcctttt tacccatttt tgtgtatgga atgtggaaag ccaaggcgtc
     9361 ataatctgcg aaatctaaac cagttctctt tttatgttcg tcccaaactt gagcgaatga
     9421 ttgtatgtag gtttcgttag acaaaggacc atcaaccata gggtatggat gacctgttgg
     9481 tctccagaaa tcatagatgt cttgagtcaa cataacgttg tcttccttca aagccaagat
     9541 tctaggttct gaggcgacca acattgcaac agcaccggca ccttgagttg gttcaccacc
     9601 agaatttaaa ccgtatttag caatatcggc tgcaactacc aatacctttt tgtcagggtg
     9661 taaggcgaca tgattctttg ctaattgcaa acctgctgta gcaccataac atgcttcttt
     9721 gatttcgaaa cttctagcaa atggttgtat acccatcaat ctgtgtaaga caacagcggc
     9781 tgctttggat tcatcaattg aagattcagt acctacaatg accatatcga ttgcttcctt
     9841 gtcttcttta gttaaaatgg cttcagcggc gtttgcagcg aaggtaacta tatcttggga
     9901 aataggattt acagccattt ggtcttgacc aataccgata tgaaatttac cagggtctac
     9961 gtttctggct tctgctaatg ctgtcatgtc aatgtaataa ggagggacga aaaaggagat
    10021 tttgtcaata ccgatggtca ttttgaattc tttatgtgat gattgattga ttgattgtac
    10081 agtttgtttt tcttaatatc tatttcgatg acttctatat gatattgcac taacaagaag
    10141 atattataat gcaattgata caagacaagg agttatttgc ttctctttta tatgattctg
    10201 acaatccata ttgcgttggt agtctttttt gctggaacgg ttcagcggaa aagacgcatc
    10261 gctctttttg cttctagaag aaatgccagc aaaagaatct cttgacagtg actgacagca
    10321 aaaatgtctt tttctaacta gtaacaaggc taagatatca gcctgaaata aagggtggtg
    10381 aagtaataat taaatcatcc gtataaacct atacacatat atgaggaaaa ataatacaaa
    10441 ag
//
