LOCUS       sequence-190181-       12964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the lower-part mevalonate pathway; overexpressing yeast 
            mevalonate pathway genes under the control of consitutive promoters.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers 
            CE
  TITLE     A squalene synthase protein degradation method for improved 
            sesquiterpene production in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. 
            Epub 2016 Dec 8.
  PUBMED    27939849
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-01"; volume: "39:209-219. doi"; pages: " 
            10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8"
COMMENT     Sequence Label: pPMVAd3
FEATURES             Location/Qualifiers
     source          1..12964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5042..5053
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(8111..8699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8191..8210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8870..9661,9662..9730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9493..9512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9731..9835)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9862..11204
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10825..10872)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11245..11263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11301..11323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11423..11442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11463..11744
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             11745..12419
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tccgtacatc ttagacaccc ttttcttttt ttcntttttt tttttttttt ttggcttccg
       61 aagtgccgac aactaaagaa ttatcgcatt ttttttgaat acatcatact atacgataga
      121 acgcattgaa acttttccca tctcaaaatc cagggacaat agtatgggat cgatattatt
      181 atcaagacat aagtattgtg atgcgcacgt tgaaattcac tatttttttt ttttatatct
      241 gtttttcaat acagtgaaca cgataactga aagaaacaat gtcattaccg ttcttaactt
      301 ctgcaccggg aaaggttatt atttttggtg aacactctgc tgtgtacaac aagcctgccg
      361 tcgctgctag tgtgtctgcg ttgagaacct acctgctaat aagcgagtca tctgcaccag
      421 atactattga attggacttc ccggacatta gctttaatca taagtggtcc atcaatgatt
      481 tcaatgccat caccgaggat caagtaaact cccaaaaatt ggccaaggct caacaagcca
      541 ccgatggctt gtctcaggaa ctcgttagtc ttttggatcc gttgttagct caactatccg
      601 aatccttcca ctaccatgca gcgttttgtt tcctgtatat gtttgtttgc ctatgccccc
      661 atgccaagaa tattaagttt tctttaaagt ctactttacc catcggtgct gggttgggct
      721 caagcgcctc tatttctgta tcactggcct tagctatggc ctacttgggg gggttaatag
      781 gatctaatga cttggaaaag ctgtcagaaa acgataagca tatagtgaat caatgggcct
      841 tcataggtga aaagtgtatt cacggtaccc cttcaggaat agataacgct gtggccactt
      901 atggtaatgc cctgctattt gaaaaagact cacataatgg aacaataaac acaaacaatt
      961 ttaagttctt agatgatttc ccagccattc caatgatcct aacctatact agaattccaa
     1021 ggtctacaaa agatcttgtt gctcgcgttc gtgtgttggt caccgagaaa tttcctgaag
     1081 ttatgaagcc aattctagat gccatgggtg aatgtgccct acaaggctta gagatcatga
     1141 ctaagttaag taaatgtaaa ggcaccgatg acgaggctgt agaaactaat aatgaactgt
     1201 atgaacaact attggaattg ataagaataa atcatggact gcttgtctca atcggtgttt
     1261 ctcatcctgg attagaactt attaaaaatc tgagcgatga tttgagaatt ggctccacaa
     1321 aacttaccgg tgctggtggc ggcggttgct ctttgacttt gttacgaaga gacattactc
     1381 aagagcaaat tgacagtttc aaaaagaaat tgcaagatga ttttagttac gagacatttg
     1441 aaacagactt gggtgggact ggctgctgtt tgttaagcgc aaaaaatttg aataaagatc
     1501 ttaaaatcaa atccctagta ttccaattat ttgaaaataa aactaccaca aagcaacaaa
     1561 ttgacgatct attattgcca ggaaacacga atttaccatg gacttcataa tagtgacaca
     1621 tttcttaaca gatggctgaa aaattttttt tttttttttg gttttgcttt gtatacacat
     1681 gtatctattt ttataaagat gaaatatata cgtctaagag ctaaaatgaa aaactatact
     1741 aatcacttat atctattctg gtgattcatc atcttcagct tctgtatccg tatcgatatc
     1801 agtataatcg ctagcatcac tttcatcatc actttcggtt tcgtcgtcat cagtagagta
     1861 ctcatcatcc tcctcagaat acacctctac ttgttccact gcaggagaat cacgaagagc
     1921 tccagaggag tacacacggg acttgatcga acagatcgtg ttacagttgc gctcgcaaag
     1981 aatgaaaatg gttcagacaa aggatcagtt cctatttatc taccatgctg ccaagtatct
     2041 taacagtctt tccgtgaacc aatagacagc tatataaaag ttcctaattg tgcatttttt
     2101 caataacaat acttattcat ccttataatt atattctagc ttcgttgtca tgggaacata
     2161 gcccatacac cgcagttatt tatgatcatt tcgaacggga agtatggatg aatctttttt
     2221 tttttttttt tatagcacgc aactgaaaaa aaaaaagaaa aatttttcat cttcgctcga
     2281 cgtttctttt gtagtactca tctcttttta tataaagatt aattagttat tgtcgctttg
     2341 cttttccttc tttaaaaaat gtttcttgct tttggatttt cagatgtccc aagatcatta
     2401 cagtatttta attgaacaaa atgtcagagt tgagagcctt cagtgcccca gggaaagcgt
     2461 tactagctgg tggatattta gttttagatc cgaaatatga agcatttgta gtcggattat
     2521 cggcaagaat gcatgctgta gcccatcctt acggttcatt gcaagagtct gataagtttg
     2581 aagtgcgtgt gaaaagtaaa caatttaaag atggggagtg gctgtaccat ataagtccta
     2641 aaactggctt cattcctgtt tcgataggcg gatctaagaa ccctttcatt gaaaaagtta
     2701 tcgctaacgt atttagctac tttaagccta acatggacga ctactgcaat agaaacttgt
     2761 tcgttattga tattttctct gatgatgcct accattctca ggaggacagc gttaccgaac
     2821 atcgtggcaa cagaagattg agttttcatt cgcacagaat tgaagaagtt cccaaaacag
     2881 ggctgggctc ctcggcaggt ttagtcacag ttttaactac agctttggcc tccttttttg
     2941 tatcggacct ggaaaataat gtagacaaat atagagaagt tattcataat ttatcacaag
     3001 ttgctcattg tcaagctcag ggtaaaattg gaagcgggtt tgatgtagcg gcggcagcat
     3061 atggatctat cagatataga agattcccac ccgcattaat ctctaatttg ccagatattg
     3121 gaagtgctac ttacggcagt aaactggcgc atttggttaa tgaagaagac tggaatataa
     3181 cgattaaaag taaccattta ccttcgggat taactttatg gatgggcgat attaagaatg
     3241 gttcagaaac agtaaaactg gtccagaagg taaaaaattg gtatgattcg catatgccgg
     3301 aaagcttgaa aatatataca gaactcgatc atgcaaattc tagatttatg gatggactat
     3361 ctaaactaga tcgcttacac gagactcatg acgattacag cgatcagata tttgagtctc
     3421 ttgagaggaa tgactgtacc tgtcaaaagt atcctgagat cacagaagtt agagatgcag
     3481 ttgccacaat tagacgttcc tttagaaaaa taactaaaga atctggtgcc gatatcgaac
     3541 ctcccgtaca aactagctta ttggatgatt gccagacctt aaaaggagtt cttacttgct
     3601 taatacctgg tgctggtggt tatgacgcca ttgcagtgat tgctaagcaa gatgttgatc
     3661 ttagggctca aaccgctgat gacaaaagat tttctaaggt tcaatggctg gatgtaactc
     3721 aggctgactg gggtgttagg aaagaaaaag atccggaaac ttatcttgat aaataatagt
     3781 gatcgaattt acgtagccca atctaccact tttttttttc attttttaaa gtgttatact
     3841 tagttatgct ctaggataat gaactacttt tttttttttt tttttactgt tatcataaat
     3901 atatatacct tattgttgtt tgcaaccgtc ggttaattcc ttatcaaggt tccccaagtt
     3961 cggatcatta ccatcaattt ccaacatttt catgagttct tcttcttcat taccgtgttt
     4021 tagggggctg ttcgcacttc taatagggct atcaccaagc tgttctaatt cgtccaaaag
     4081 ttactagttg ccattacgta cggtaggcaa aggcaaacaa gaatagcact ttttcacttg
     4141 ctgctttttt ttgtgaatga agtttatcgt atattctgtt agtgccgctc gtatcagcca
     4201 cttacgttat cattctaaag taagtatacg aaatgtgcaa accttagtag acgttcaaga
     4261 attgctcgat aattgcgaac aaaccagctg ctaagccggg aagttaagtc ctcaacacag
     4321 aaatgatgca aacaatttat taaattcagt ggtaatgcaa cagcaagagg aaaggtggag
     4381 ggattaacgc atttcagaca gctttatagg gggaaagaaa gcactcgcaa acttgctgcc
     4441 tgttcgcagt cattggttgc aaaaactaaa ctctactcac gcacactgga atgaatggca
     4501 atattctttt ttaggttaac cggccggaca gtaatatagt aatcgttttg tacgtttttc
     4561 aagaagcgac gcacaactgt tttccatttt tttttttttt tttcagtgat catcgtccat
     4621 gaaaaaaatt tttcatttgt ctctttcgtg cttcctggat atataaaata cgatttattt
     4681 agttgtcttt gtcaatcctc atctttcttt actcattatt tcatttcggt tttgtcatct
     4741 ctagaacaac acagttacta caacaatcaa acatgaccgt ttacacagca tccgttaccg
     4801 cacccgtcaa catcgcaacc cttaagtatt gggggaaaag ggacacgaag ttgaatctgc
     4861 ccaccaattc gtccatatca gtgactttat cgcaagatga cctcagaacg ttgacctctg
     4921 cggctactgc acctgagttt gaacgcgaca ctttgtggtt aaatggagaa ccacacagca
     4981 tcgacaatga aagaactcaa aattgtctgc gcgacctacg ccaattaaga aaggaaatgg
     5041 aatcgaagga cgcctcattg cccacattat ctcaatggaa actccacatt gtctccgaaa
     5101 ataactttcc tacagcagct ggtttagctt cctccgctgc tggctttgct gcattggtct
     5161 ctgcaattgc taagttatac caattaccac agtcaacttc agaaatatct agaatagcaa
     5221 gaaaggggtc tggttcagct tgtagatcgt tgtttggcgg atacgtggcc tgggaaatgg
     5281 gaaaagctga agatggtcat gattccatgg cagtacaaat cgcagacagc tctgactggc
     5341 ctcagatgaa agcttgtgtc ctagttgtca gcgatattaa aaaggatgtg agttccactc
     5401 agggtatgca attgaccgtg gcaacctccg aactatttaa agaaagaatt gaacatgtcg
     5461 taccaaagag atttgaagtc atgcgtaaag ccattgttga aaaagatttc gccacctttg
     5521 caaaggaaac aatgatggat tccaactctt tccatgccac atgtttggac tctttccctc
     5581 caatattcta catgaatgac acttccaagc gtatcatcag ttggtgccac accattaatc
     5641 agttttacgg agaaacaatc gttgcataca cgtttgatgc aggtccaaat gctgtgttgt
     5701 actacttagc tgaaaatgag tcgaaactct ttgcatttat ctataaattg tttggctctg
     5761 ttcctggatg ggacaagaaa tttactactg agcagcttga ggctttcaac catcaatttg
     5821 aatcatctaa ctttactgca cgtgaattgg atcttgagtt gcaaaaggat gttgccagag
     5881 tgattttaac tcaagtcggt tcaggcccac aagaaacaaa cgaatctttg attgacgcaa
     5941 agactggtct accaaaggaa taatagtgaa cagaagacgg gagacactag cacacaactt
     6001 gacaaaggat ctacttctcg attgacgaca ctagtaggtg tcaaatttag tgtcatttat
     6061 gattttttgt aataggatat aaatatatac ggcgctccaa ataatgcagt tgccccaaaa
     6121 acaccacgga acctcatctg ttctcgtact ttgttgtgac aaagtagctc actgccttat
     6181 tatcacattt tcattatgca acgcttcgga aaatacgatg ttgaaaatgc ctctagagat
     6241 gaaaaacaat cgtaaaaggg tcctgcgtaa ttgaaacagt cgacatacat aacattttaa
     6301 gataagcaag tgaatgttga ttcccgtttc ttagtcaaca cttctttcta ttttacccgg
     6361 tcgttaccct attaaaaaaa caacttacaa tcattgttcg ccccttccat acttactgcc
     6421 actcgcaaaa gggcccaacc agggcaatta cgtatcaaaa aatcatgaca ggctgggtaa
     6481 taaatattcg tgaagaaaga agaaattaaa aaaagaaacg aagaagcaaa aaaaagaaaa
     6541 gactccgttt aatcactttc aaccgcggtt tatccggccc cacccatgca taaccctaaa
     6601 ttattagatc acttagcacg tgaaaaagaa acgtttttaa tgtttttttt ttttttttct
     6661 ttttcttttt ttgcgttggt gaaaattttt tcgcttcctc gagtataatt atctcatctc
     6721 atctttcata taagataaga agttttataa aaaccttttg catcaaaatt ttgtagaata
     6781 tctctttttc ttacgctctc tttctttcct taattgtttt ctaaagaacc gtgtattttt
     6841 ctagttcgaa tccatcgata acattaaaac atgactgccg acaacaatag tatgccccat
     6901 ggtgcagtat ctagttacgc caaattagtg caaaaccaaa cacctgaaga cattttggaa
     6961 gagtttcctg aaattattcc attacaacaa agacctaata cccgatctag tgagacgtca
     7021 aatgacgaaa gcggagaaac atgtttttct ggtcatgatg aggagcaaat taagttaatg
     7081 aatgaaaatt gtattgtttt ggattgggac gataatgcta ttggtgccgg taccaagaaa
     7141 gtttgtcatt taatggaaaa tattgaaaag ggtttactac atcgtgcatt ctccgtcttt
     7201 attttcaatg aacaaggtga attactttta caacaaagag ccactgaaaa aataactttc
     7261 cctgatcttt ggactaacac atgctgctct catccactat gtattgatga cgaattaggt
     7321 ttgaagggta agctagacga taagattaag ggcgctatta ctgcggcggt gagaaaacta
     7381 gatcatgaat taggtattcc agaagatgaa actaagacaa ggggtaagtt tcacttttta
     7441 aacagaatcc attacatggc accaagcaat gaaccatggg gtgaacatga aattgattac
     7501 atcctatttt ataagatcaa cgctaaagaa aacttgactg tcaacccaaa cgtcaatgaa
     7561 gttagagact tcaaatgggt ttcaccaaat gatttgaaaa ctatgtttgc tgacccaagt
     7621 tacaagttta cgccttggtt taagattatt tgcgagaatt acttattcaa ctggtgggag
     7681 caattagatg acctttctga agtggaaaat gacaggcaaa ttcatagaat gctataatag
     7741 tgagctggtt gatggaaaat ataattttat tgggcaaact tttgtttatc tgatgtgttt
     7801 tatactatta tctttttaat taatgattct atatacaaaa ctgtatattt tttctttaac
     7861 caattttttt ttatagacct agagctgtac ttttattctg ctatcaagca aacccctacc
     7921 ccctcttctc aatcctcccc tcaggcagaa cttatctacc tgtatcaagg agcggacgag
     7981 ggagtcctag ttgttctacg tataccaatg ctagcagctt acataggtgg tggcactacc
     8041 agcggccgca catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9841 ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc
     9901 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     9961 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
    10021 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
    10081 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
    10141 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
    10201 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
    10261 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
    10321 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
    10381 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
    10441 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
    10501 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
    10561 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
    10621 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
    10681 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
    10741 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
    10801 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
    10861 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
    10921 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
    10981 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
    11041 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
    11101 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
    11161 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt
    11221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    11281 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    11341 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    11401 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataga
    11461 tcaacgacat tactatatat ataatatagg aagcatttaa tagaacagca tcgtaatata
    11521 tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt tattgaaaaa
    11581 tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa
    11641 ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
    11701 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
    11761 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
    11821 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
    11881 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
    11941 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt
    12001 tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc
    12061 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
    12121 gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
    12181 cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa
    12241 ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg
    12301 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
    12361 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
    12421 ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg
    12481 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
    12541 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
    12601 aatcggcaaa atcccttata acctgcaggg ccttgcggag gataaccacc aacgaactca
    12661 aatacctacc gcgtaccttc ctacggttat gtacttttgc actgctattg ctttgagatg
    12721 ctatgagaac tgccgtcaaa attgggtggc aaagagggat agcttcactg ccaatcacta
    12781 cactctgttc tgaaggaggg gaattactgt tgtctcataa aaaaacacct ttgaaacact
    12841 ttcactcata gagaagccaa gaactctgat gagttcttta tttgttgccg ctaactttat
    12901 cttatcttct ccattgtaat aattactatt ctttttactt gcctgttttc catcccaatg
    12961 aaga
//
