LOCUS       sequence-190182-       14813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the upper-part mevalonate pathway; overexpressing 
            codon-optimized Enterococcus faecalis mevalonate pathway genes under
            the control of galactose-inducible promoters; integrated into genome
            HMG2 locus; overexpressing HMG2 K6R..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 14813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pIMVAu1
FEATURES             Location/Qualifiers
     source          1..14813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1549..2137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1629..1648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2308..3099,3100..3168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2931..2950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3169..3273)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3300..4642
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4263..4310)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4683..4701
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4739..4761)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4861..4880
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4901..5182
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5183..5857
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             complement(6375..7037)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(7038..7225)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        complement(8709..9150)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(8715..8738)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    9033..9150
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 agacagagct ttagcaattt tgaacgattt gagaaaacaa taatgatagt attgttcgtt
       61 ttttatcata gtttttcaaa tcttcatatg aatactaggt agaaatttgg cttatcagta
      121 cgaatgaatt acagtttgac tgatttgtac acgagaattg acgtataact tcttttgaaa
      181 gtctttgaaa ttacatggta gtacatacat atatatatat atatatatat gttatattat
      241 tgttaattaa atattattat gcgtattttc cttttcttta ttagtatagt attaatgaca
      301 gtaataataa taataatagt aacaatatct cttttttttt ttcagtgagc ttttattttt
      361 ttttcattgc tcttcttttg gcctcttttg tttttttctt gatttcctcc agtttcatct
      421 gttttttctt tggatcagat acaaaatctg gtttgaacgc gagctctttg ccagcttact
      481 atccttcttg aaaatatgca ctctatatct tttagttctt aattgcaaca catagatttg
      541 ctgtataacg aattttatgc tattttttaa atttggagtt cagtgataaa agtgtcacag
      601 cgaatttcct cacatgtagg gaccgaattg tttacaagtt ctctgtacca ccatggagac
      661 atcaaagatt gaaaatctat ggaaagatat ggacggtagc aacaagaata tagcacgagc
      721 cgcgaagttc atttcgttac ttttgatatc gctcacaact attgcgaagc gcttcagtga
      781 aaaaatcata aggaaaagtt gtaaatatta ttggtagtat tcgtttggta aagtagaggg
      841 ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct ctccttttgg
      901 aaagctatac ttcggagcac tgttgagcga aggctcatta gatatatttt ctgtcatttt
      961 ccttaaccca aaaataaggg aaagggtcca aaaagcgctc ggacaactgt tgaccgtgat
     1021 ccgaaggact ggctatacag tgttcacaaa atagccaagc tgaaaataat gtgtagctat
     1081 gttcagttag tttggctagc aaagatataa aagcaggtcg gaaatattta tgggcattat
     1141 tatgcagagc atcaacatga taaaaaaaaa cagttgaata ttccctcaaa aatgtcactt
     1201 cccttaagaa cgatagtaca tttggtaaag ccctttgctt gcactgctag gtttagtgcg
     1261 agatacccaa tccacgtcat tgttgttgct gttttattga gtgccgctgc ttatctatcc
     1321 gtgacacaat cttaccttaa cgaatggaag ctggactcta atcagtattc tacatactta
     1381 agcataaagc cggatgagtt gtttgaaaaa tgcacacact actataggtc tcctgtgtct
     1441 gatacatgga agttactcag ctctaaagaa gccgccgata tttaaataca tgtgagcaaa
     1501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1981 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3301 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     3361 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     3421 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     3481 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     3541 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     3601 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     3661 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     3721 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     3781 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     3841 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     3901 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     3961 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     4021 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     4081 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     4141 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     4201 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
     4261 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     4321 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     4381 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     4441 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     4501 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     4561 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     4621 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
     4681 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4741 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4801 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4861 cggcatcaga gcagattgta ctgagagtgc accatagatc aacgacatta ctatatatat
     4921 aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg
     4981 ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa
     5041 tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa
     5101 aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa
     5161 gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt
     5221 ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc
     5281 tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc
     5341 aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt
     5401 ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg
     5461 cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg
     5521 tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc
     5581 ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga
     5641 acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt
     5701 acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa
     5761 tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa
     5821 caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt
     5881 taagtattgt ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag
     5941 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     6001 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttattta
     6061 aatagaccat tgatcgttag agacgagcgc attgttttgt tgaatccctt tcaacagcgc
     6121 gacaaaagtt aatcgagaac aataagatgt tgcattcaag ggttagtcaa acatatcaga
     6181 aggccgtttt ctaaatcacc tcaagaacct accacagaaa gcgcgcctcg ttcagaatga
     6241 cacgtataga atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact
     6301 tactgacatt cataggtata catatataca catgtatata tatcgtatgc tgcagcttta
     6361 aataatcggt gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg
     6421 ggcgaggtgg cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga
     6481 tgatcattct tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag
     6541 ctttcaagaa aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa
     6601 ccaagttcga caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc
     6661 tcatccaaag gcgcaaatcc tgatccaaac ctttttactc cacgcacggc ccctagggcc
     6721 tctttaaaag cttgaccgag agcaatcccg cagtcttcag tggtgtgatg gtcgtctatg
     6781 tgtaagtcac caatgcactc aacgattagc gaccagccgg aatgcttggc cagagcatgt
     6841 atcatatggt ccagaaaccc tatacctgtg tggacgttaa tcacttgcga ttgtgtggcc
     6901 tgttctgcta ctgcttctgc ctctttttct gggaagatcg agtgctctat cgctagggga
     6961 ccacccttta aagagatcgc aatctgaatc ttggtttcat ttgtaatacg ctttactagg
     7021 gctttctgct ctgtcatctt tgccttcgtt tatcttgcct gctcattttt tagtatattc
     7081 ttcgaagaaa tcacattact ttatataatg tataattcat tatgtgataa tgccaatcgc
     7141 taagaaaaaa aaagagtcat ccgctaggtg gaaaaaaaaa aatgaaaatc attaccgagg
     7201 cataaaaaaa tatagagtgt actaggatcc tactgaaatt catgacacaa ataacgacat
     7261 attttgttaa agatttgtcg gaggtagaac ttcatgggca catgcattgt attgataaaa
     7321 aaatgctgga caagtcgaaa ttcatgtatt tatacgaaga gaaaagcaac gaggaagtga
     7381 aagcagcttc tcctcaaaag gagaagatag tagataatat cattggccta ttatcaaatg
     7441 atgaagaaca ttaacatgaa ttggaaaaag aaaaaattgc atgtaaatat atacatacgt
     7501 agaattttat acttaggcta cagatcaaag ctatcattaa tttctgtatg atctaactgt
     7561 gttattgata gcactgatgg agtacttcaa ttcatcttct aaagtttgat cgatgtcagt
     7621 atccaaggtt tcagcaaaca tggcttcgta ttctgcaata gataattcgg ttctgttatc
     7681 taacaaagcc aagtgtgttt ccttttgcaa atggttttgg taaccagcga ccaattcacc
     7741 agtgaaaaat tcggcaactg cacctgaacc ataagaaaac aaaccgattt ggttacctgc
     7801 ggtcaatgta gtagcgtttt ctaacaaact tatcaaacct aagtacaagg aacctgtata
     7861 caaattacca actcttctac tgtatatgat ggattcttcg tatctggcta atattctttc
     7921 ttgttctgct tcggtttgat ctgaaatctt tgctaacaaa gcctttttac ccatttttgt
     7981 gtatggaatg tggaaagcca aggcgtcata atctgcgaaa tctaaaccag ttctcttttt
     8041 atgttcgtcc caaacttgag cgaatgattg tatgtaggtt tcgttagaca aaggaccatc
     8101 aaccataggg tatggatgac ctgttggtct ccagaaatca tagatgtctt gagtcaacat
     8161 aacgttgtct tccttcaaag ccaagattct aggttctgag gcgaccaaca ttgcaacagc
     8221 accggcacct tgagttggtt caccaccaga atttaaaccg tatttagcaa tatcggctgc
     8281 aactaccaat acctttttgt cagggtgtaa ggcgacatga ttctttgcta attgcaaacc
     8341 tgctgtagca ccataacatg cttctttgat ttcgaaactt ctagcaaatg gttgtatacc
     8401 catcaatctg tgtaagacaa cagcggctgc tttggattca tcaattgaag attcagtacc
     8461 tacaatgacc atatcgattg cttccttgtc ttctttagtt aaaatggctt cagcggcgtt
     8521 tgcagcgaag gtaactatat cttgggaaat aggatttaca gccatttggt cttgaccaat
     8581 accgatatga aatttaccag ggtctacgtt tctggcttct gctaatgctg tcatgtcaat
     8641 gtaataagga gggacgaaaa aggagatttt gtcaataccg atggtcattt tgaattctat
     8701 agttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt
     8761 tatgacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg
     8821 ttatgcagct tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt
     8881 aattacccca gaaataaggc taaaaaacta atcgcattat tatcctatgg ttgttaattt
     8941 gattcgttga tttgaaggtt tgtggggcca ggttactgcc aatttttcct cttcataacc
     9001 ataaaagcta gtattgtaga atctttattg ttcggagcag tgcggcgcga ggcacatctg
     9061 cgtttcagga acgcgaccgg tgaagaccag gacgcacgga ggagagtctt ccgtcggagg
     9121 gctgtcgccc gctcggcggc ttctaatccg tacttcaata tagcaatgag cagttaagcg
     9181 tattactgaa agttccaaag agaaggtttt tttaggctaa gataatgggg ctctttacat
     9241 ttccacaaca tataagtaag attagatatg gatatgtata tggtggtatt gccatgtaat
     9301 atgattatta aacttctttg cgtccatcca aaaaaaaagt aagaattttt gaaaattcaa
     9361 tataagtcga caaaatgaca atcaaggaac ataaagtagt ttatgaagct cacaacgtaa
     9421 aggctcttaa ggctcctcaa catttttaca acagccaacc cggcaagggt tacgttactg
     9481 atatgcaaca ttatcaagaa atgtatcaac aatctatcaa tgagccagaa aaattctttg
     9541 ataagatggc taaggaatac ttgcattggg atgctccata caccaaagtt caatctggtt
     9601 cattgaacaa tggtgatgtt gcatggtttt tgaacggtaa attgaatgca tcatacaatt
     9661 gtgttgacag acatgccttt gctaatcccg acaagccagc tttgatctat gaagctgatg
     9721 acgaatccga caacaaaatc atcacatttg gtgaattact cagaaaagtt tcccaaatcg
     9781 ctggtgtctt aaaaagctgg ggcgttaaga aaggtgacac agtggctatc tatttgccaa
     9841 tgattccaga agcggtcatt gctatgttgg ctgtggctcg tattggtgct attcactctg
     9901 ttgtctttgc tgggttctcc gctggttcgt tgaaagatcg tgtcgttgac gctaattcta
     9961 aagtggtcat cacttgtgat gaaggtaaaa gaggtggtaa gaccatcaac actaaaaaaa
    10021 ttgttgacga aggtttgaac ggagtcgatt tggtttcccg tatcttggtt ttccaaagaa
    10081 ctggtactga aggtattcca atgaaggccg gtagagatta ctggtggcat gaggaggccg
    10141 ctaagcagag aacttaccta cctcctgttt catgtgacgc tgaagatcct ctatttttat
    10201 tatacacttc cggttccact ggttctccaa agggtgtcgt tcacactaca ggtggttatt
    10261 tattaggtgc cgctttaaca actagatacg tttttgatat tcacccagaa gatgttctct
    10321 tcactgccgg tgacgtcggc tggatcacgg gtcacaccta tgctctatat ggtccattaa
    10381 ccttgggtac cgcctcaata attttcgaat ccactcctgc ctacccagat tatggtagat
    10441 attggagaat tatccaacgt cacaaggcta cccatttcta tgtggctcca actgctttaa
    10501 gattaatcaa acgtgtaggt gaagccgaaa ttgccaaata tgacacttcc tcattacgtg
    10561 tcttgggttc cgtcggtgaa ccaatctctc cagacttatg ggaatggtat catgaaaaag
    10621 tgggtaacaa aaactgtgtc atttgtgaca ctatgtggca aacagagtct ggttctcatt
    10681 taattgctcc tttggcaggt gctgtcccaa caaaacctgg ttctgctacc gtgccattct
    10741 ttggtattaa cgcttgtatc attgaccctg ttacaggtgt ggaattagaa ggtaatgatg
    10801 tcgaaggtgt ccttgccgtt aaatcaccat ggccatcaat ggctagatct gtttggaacc
    10861 accacgaccg ttacatggat acttacttga aaccttatcc tggtcactat ttcacaggtg
    10921 atggtgctgg tagagatcat gatggttact actggatcag gggtagagtt gacgacgttg
    10981 taaatgtttc cggtcataga ttatccacat cagaaattga agcatctatc tcaaatcacg
    11041 aaaacgtctc ggaagctgct gttgtcggta ttccagatga attgaccggt caaaccgtcg
    11101 ttgcatatgt ttccctaaaa gatggttatc tacaaaacaa cgctactgaa ggtgatgcag
    11161 aacacatcac accagataat ttacgtagag aattgatctt acaagttagg ggtgagattg
    11221 gtcctttcgc ctcaccaaaa accattattc tagttagaga tctaccaaga acaaggtcag
    11281 gaaagattat gagaagagtt ctaagaaagg ttgcttctaa cgaagccgaa cagctaggtg
    11341 acctaactac tttggccaac ccagaagttg tacctgccat catttctgct gtagagaacc
    11401 aatttttctc tcaaaaaaag aaataactta aatgagaaaa atttcgtaat gagataaaat
    11461 ttcgctcctt ttctgttttc tattttctat tttcccaact tttgctctat tcagttataa
    11521 attactattt atccatcagt taaaaaacaa gatcttttac tggtcagcta ggaaagcgaa
    11581 aatacaaaga ctttatgcac ttagtgatat atatgtatag atatatccat ttttacgcac
    11641 ttatcatata tcttagttat ctaaatacaa tctagttatt cgtacacaat cgcccctgtt
    11701 atccctatag tgggaataaa gtaatgcatg ctgtactaat ccaaggaggt ttacggacca
    11761 gaggaacttt ccagattcag atcacagcaa tataggactg gaaaacatca ggtagccgca
    11821 ctcaacttgt aactggcaac tactttgcat taaactccaa ttaaatgcgg tagaatcttt
    11881 tcagaaaagg tattcaacgt caattcggaa agcttccttc cggaatggct taagtaggtt
    11941 gcaatttctt tttctattag tagctaaaaa tgggtcacgt gatctatatt cgaaaggggc
    12001 ggttgcctca ggaaggcacc ggcggtcttt cgtccgtgcg gagatatctg cgccgttcag
    12061 gggtccatgt gccttggacg atattaaggc agaaggcagt atcggggcgg atcactccga
    12121 accgagatta gttaagccct tcccatctca agatggggag caaatggcat tatactcctg
    12181 ctagaaagtt aactgtgcac atattcttaa attatacaat gttctggaga gctattgttt
    12241 aaaaaacaaa catttcgcag gctaaaatgt ggagatagga ttagttttgt agacatatat
    12301 aaacaatcag taattggatt gaaaatttgg tgttgtgaat tgctcttcat tatgcacctt
    12361 attcaattat catcaagaat agcaatagtt aagtaaacac aagattaaca taataaaaaa
    12421 aataattctt tcataactag taaaatgaag actgtagtaa taatagacgc attgagaaca
    12481 ccaatcggta aatataaagg tagtttgtcc caagtttccg ctgtagactt aggtactcat
    12541 gttactacac aattgttgaa gagacactcc acaatcagtg aagaaatcga tcaagtcata
    12601 ttcggtaacg tattacaagc tggtaacggt caaaacccag ctagacaaat agcaatcaat
    12661 tctggtttgt cacatgaaat tcctgctatg acagtaaacg aagtttgtgg ttcaggcatg
    12721 aaagccgtaa ttttggctaa gcaattgatc caattgggtg aagccgaagt tttgatcgct
    12781 ggtggtatag aaaacatgag tcaagctcca aaattacaaa gattcaatta cgaaactgaa
    12841 tcttacgatg cacctttctc ttcaatgatg tatgatggtt taactgacgc attttctggt
    12901 caagccatgg gtttgacagc agaaaatgta gccgaaaagt accatgttac cagagaagaa
    12961 caagatcaat tttccgttca cagtcaattg aaagctgcac aagcacaagc cgaaggtatt
    13021 ttcgctgacg aaatagcacc attagaagtt tctggtacat tggtcgaaaa ggatgaaggt
    13081 ataagaccta actccagtgt tgaaaaattg ggtactttga agacagtttt caaggaagac
    13141 ggtacagtca ccgccggtaa tgcttctacc attaacgatg gtgctagtgc attgataatc
    13201 gcttctcaag aatatgccga agctcatggt ttgccatact tagctatcat tagagatagt
    13261 gtagaagttg gtattgaccc agcatatatg ggtatttctc ctataaaagc catccaaaag
    13321 ttgttggcta gaaaccaatt gaccactgaa gaaatcgatt tgtacgaaat taacgaagca
    13381 tttgccgcta cttcaatcgt tgtccaaaga gaattggcat tgcctgaaga aaaggttaac
    13441 atctatggtg gtggtatctc cttaggtcac gcaattggtg ccaccggtgc tagattgttg
    13501 acttccttga gttaccaatt gaaccaaaag gaaaagaaat acggtgttgc ttctttgtgc
    13561 attggtggtg gtttgggttt agcaatgttg ttagaaagac cacaacaaaa gaaaaattca
    13621 agattctacc aaatgtcccc tgaagaaaga ttggcatcat tgttgaacga aggtcaaatt
    13681 tccgccgata ctaagaaaga atttgaaaac accgcattgt cttcacaaat cgccaaccat
    13741 atgatcgaaa accaaatctc tgaaacagaa gttccaatgg gtgtcggttt gcacttaact
    13801 gtagatgaaa cagactattt ggttccaatg gctaccgaag aacctagtgt tatcgcagcc
    13861 ttgtctaatg gtgcaaaaat tgcccaaggt tttaagactg ttaaccaaca aagattgatg
    13921 agaggtcaaa ttgtattcta cgatgttgca gacgccgaat cattgatcga tgaattacaa
    13981 gttagagaaa ctgaaatctt tcaacaagct gaattatctt acccatcaat cgttaagaga
    14041 ggtggtggtt tgagagattt gcaatacaga gcttttgacg aatccttcgt cagtgtagat
    14101 ttcttggttg atgtcaaaga cgctatgggt gcaaatattg tcaacgctat gttagaaggt
    14161 gtagcagaat tgtttagaga atggttcgct gaacaaaaga ttttgttttc tatcttgtca
    14221 aactatgcta cagaatctgt agttaccatg aaaactgcaa ttccagtttc cagattatct
    14281 aagggttcta acggtagaga aatcgctgaa aagattgtct tagcctcaag atatgcttcc
    14341 ttggaccctt acagagcagt tactcataat aagggtataa tgaacggtat cgaagcagtc
    14401 gtattggcca ccggtaatga tactagagcc gtttctgctt catgtcacgc tttcgcagtc
    14461 aaggaaggta gatatcaagg tttgacatca tggaccttag atggtgaaca attgattggt
    14521 gaaatatccg ttccattggc cttagctact gtcggtggtg ctacaaaagt attacctaag
    14581 agtcaagctg ctgctgattt gttagctgta acagacgcaa aagaattgtc aagagttgtc
    14641 gctgctgttg gtttggccca aaatttggcc gctttaagag ctttggtctc agaaggtatt
    14701 caaaagggtc atatggcatt acaagccaga tccttggcta tgacagttgg tgcaaccggt
    14761 aaagaagtcg aagcagtagc ccaacaattg aagagacaaa agactatgaa cca
//
