LOCUS       sequence-190192-        4149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             524..993
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1058..1415
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1826..1848
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1864..1880
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1933..1966
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2074..2208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2111..2130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3991..4020)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4097..4121)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tacgccaagc tagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa
       61 agcatttgac ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac
      121 atttaaaatg ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat
      181 gtgcatgaat gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg
      241 tggaaattac ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc
      301 gctgctgaga agccagtttg catctgtcag gatcaatttc ccattatgcc agtcatatta
      361 attactagtc aattagttga tttttatttt tgacatatac atgtgaaaga ccccacctgt
      421 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      481 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      541 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
      601 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      661 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
      721 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      781 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      841 ctcactcggc gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
      901 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
      961 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     1021 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     1081 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     1141 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     1201 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     1261 taaaatcccg atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt
     1321 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1381 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     1441 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc
     1501 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     1561 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     1621 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     1681 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     1741 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     1801 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     1861 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatccga
     1921 agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcagat cataatcagc
     1981 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2041 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2101 tacaaataag gcaatagcat cacaaatttc acaaataagg catttttttc actgcattct
     2161 agttttggtt tgtccaaact catcaatgta tcttatcatg tctggatctc aaatccctcg
     2221 gaagctgcgc ctgtcttagg ttggagtgat acatttttat cacttttacc cgtctttgga
     2281 ttaggcagta gctctgacgg ccctcctgtc ttaggttagt gaaaaatgtc actctcttac
     2341 ccgtcattgg ctgtccagct tagctcgcag gggaggtggt ctggatccct aacgcgtctg
     2401 attttcgtct tccacgtcct gcagcgcttc tttattctgc tcttcaccgt caccctgcat
     2461 gtctgaagtc catagtgtca gattatcacg taacaactgc atgataagtg tagagtcctt
     2521 atagctttct tcactcagcg tatccagttc tgcaattgca tcatcaaaag ctgcttttgc
     2581 caacctgcag gcacggtcag gggaattaag aatttcgtag tagaatacgg aaaaattgag
     2641 agcaagacct aagcgaatag gatgcgttgg tggaagttct gtcattgcaa tatcactagc
     2701 agctttataa gccactaggc tgttctccgc agcctccttc ctgtcgtttc ctgtggcaaa
     2761 ttctgccaga tacctgtggt agtccccttt cattttataa tagaaaacct tggactcgcc
     2821 agtgttagct gctggaatga ggtgtttgtc cagtacatcc agaatgtcac aacagattaa
     2881 ctttagctca gtctcaacca tttgccgata ttcccgaatc atttttagct tgtcttctcc
     2941 tcccttgttt tcttctttct gttcaatgct gctgattatt ctccaggagg ctcttctagc
     3001 tccaatcaca ttcttatatg caacagatag gaggtttctt tcttcaactg tcagctccac
     3061 atccatccct gctactttct tcattgaccc caccatttcg tcgtatcgct cagcctgctc
     3121 ggccagcttc gcctggtaca ccagatcctc tcgatcatcc atatcgatgc cccgggaggc
     3181 gtaggagatc tggccgccgc cggaggagcc gccgccatgt ttctcctgct cgaacagctc
     3241 cagagcggct ctcagctctc tgatcagcat ggcggccagg gtgttccggt ccaggttgat
     3301 gccagcctcc tgcagggtga tccagccctg gttcaccacg ctctcctcca ctcttctcat
     3361 ggccatgttg attccggctc cgatcacgat ctgagcggca tcgcctgtct tgcctgtcag
     3421 ctccaccagg atgccagcca gcttgcggtc ctggaggtac aggtcattag gccacttcac
     3481 tctcaccttg tcggctccca gctttctcag cacctcggcc atcacgattc cgatcaccag
     3541 actcagtccg atggcagcag caggtccctg ctccagtctc cagaacatgg acaggtacag
     3601 gttggctccg aatgggctga accacttccg tcctctgcct cctctgccag cctgctggta
     3661 ctcggcgatg caggcgtcgc cggacttcag ctctccgatt ctgtccagca ggtactgatt
     3721 ggtggagtcg atcacaggca gcacggccac gcttccgcca tccagctgtc ccaggatctg
     3781 cttggcgttc agcagctgga taggctcagg caggctgtag cccttgccag gcacggtgaa
     3841 cacgtccact ccccagtctc tcagtgtctg gatgtgcttg ttgatggcgg ctctgctcat
     3901 tcccagggtc tctcccagct gctcgccaga gtggaactcg ccgttggcca gcagggcgat
     3961 cagcttcagg ggcacggtgt tgtccttgtc gagatcctct tctgagatga gtttttgttc
     4021 catctcgacc gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
     4081 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggcctccc
     4141 accgtacac
//
