LOCUS       sequence-190193-        2720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e
FEATURES             Location/Qualifiers
     source          1..2720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1853..1900
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
       61 gaccgatcca gcctccgcgg aattcggccg gcctcaatta tggactacaa ggacgacgat
      121 gacaagatta acggactggc tccttacctg agcagatggg agaagctgga caacttcatc
      181 aacagacctg tgaagctgat catcggcgac aaggaaatct tcggcatctc cagaggaatc
      241 gacaagcagg gagctctgct gctggagcag gacggaatca tcaagccctg gatgggcgga
      301 gaaatctccc tgagaagcgc agagaagctc gaggcgaacc agatctccta cgcctcccgg
      361 ggcggcggct cctccggcgg cggcgtttgt ttcatgtcct ctacggaact cttctcatcc
      421 acaagagagg aaggaagctc tggctcagga cccagtttta ggtctaatca aaggaaaatg
      481 ttaaacctgc tcctggagag agacacttcc tttaccgtct gtccagatgt ccctagaact
      541 ccagtgggca aatttcttgg tgattctgca aacctaagca ttttgtctgg aggaacccca
      601 aaatgttgcc tcgatctttc gaatcttagc agtggggaga taactgccac tcagcttacc
      661 acttctgcag accttgatga aactggtcac ctggattctt caggacttca ggaagtgcat
      721 ttagctggga tgaatcatga ccagcaccta atgaaatgta gcccagcaca gcttctttgt
      781 agcactccga atggtttgga ccgtggccat agaaagagag atgcaatgtg tagttcatct
      841 gcaaataaag aaaatgacaa tggaaacttg gtggacagtg aaatgaaata tttgggcagt
      901 cccattacta ctgttccaaa attggataaa aatccaaacc taggagaaga ccaggcagaa
      961 gagatttcag atgaattaat ggagttttcc ctgaaagatc aagaagcaaa ggtgagcaga
     1021 agtggcctat atcgctcccc gtcgatgcca gagaacttga acaggccaag actgaagcag
     1081 gtggaaaaat tcaaggacaa cacaatacca gataaagtta aaaaaaagta tttttctggc
     1141 caaggaaagc tcaggaaggg cttatgttta aagaagacag tctctctgtg tgacattact
     1201 atcactcaga tgctggagga agattctaac caggggcacc tgattggtga tttttccaag
     1261 gtatgtgcgc tgccaaccgt gtcagggaaa caccaagatc tgaagtatgt caacccagaa
     1321 acagtggctg ccttactgtc ggggaagttc cagggtctga ttgagaagtt ttatgtcatt
     1381 gattgtcgct atccatatga gtatctggga ggacacatcc agggagcctt aaacttatat
     1441 agtcaggaag aactgtttaa cttctttctg aagaagccca tcgtcccttt ggacacccag
     1501 aagagaataa tcatcgtgtt ccactgtgaa ttctcctcag agaggggccc ccgaatgtgc
     1561 cgctgtctgc gtgaagagga caggtctctg aaccagtatc ctgcattgta ctacccagag
     1621 ctatatatcc ttaaaggcgg ctacagagac ttctttccag aatatatgga actgtgtgaa
     1681 ccacagagct actgccctat gcatcatcag gaccacaaga ctgagttgct gaggtgtcga
     1741 agccagagca aagtgcagga aggggagcgg cagctgcggg agcagattgc ccttctggtg
     1801 aaggacatga gcccattaat taaatagcga ttaataacga ggatctggcc gcgaagttcc
     1861 tattccgaag ttcctattct ctagaaagta taggaacttc agatccggat tagtccaatt
     1921 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
     1981 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
     2041 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctcgacttc tgttcgcgcg
     2101 cttctgctcc ccgagctcga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     2161 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     2221 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     2281 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     2341 ggccatagta cttaaagtta cattggcttc cttgaaataa acatggagta ttcagaatgt
     2401 gtcataaata tttctaattt taagatagta tctccattgg ctttctactt tttcttttat
     2461 ttttttttgt cctctgtctt ccatttgttg ttgttgttgt ttgtttgttt gtttgttggt
     2521 tggttggtta attttttttt aaagatccta cactatagtt caagctagac tattagctac
     2581 tctgtaaccc agggtgacct tgaagtcatg ggtagcctgc tgttttagcc ttcccacatc
     2641 taagattaca ggtatgagct atcatttttg gtatattgat tgattgattg attgatgtgt
     2701 gtgtgtgtga ttgtgtttgt
//
