LOCUS       sequence-190207-       12962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the lower-part mevalonate pathway; overexpressing yeast 
            mevalonate pathway genes under the control of consitutive promoters 
            or CUP1 promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 12962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pPMVAd36
FEATURES             Location/Qualifiers
     source          1..12962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3349..3360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(5999..6233)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     CDS             complement(6617..7291)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(7292..7573)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(7594..7613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7713..7735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7773..7791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      7832..9174
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8164..8211
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9201..9305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9306..9374,9375..10166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9524..9543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10337..10925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10826..10845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11153..11566
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gcaaactacc tccgtacatt catgttgcac ccacacattt atacacccag accgcgacaa
       61 attacccata aggttgtttg tgacggcgtc gtacaagaga acgtgggaac tttttaggct
      121 caccaaaaaa gaaagaaaaa atacgagttg ctgacagaag cctcaagnaa aaaaaaaatt
      181 cttcttcgac tatgctggag gcagagatga tcgagccggt agttaactat atatagctaa
      241 attggttcca tcaccttctt ttctggtgtc gctccttcta gtgctatttc tggcttttcc
      301 tatttttttt tttccatttt tctttctctc tttctaatat ataaattctc ttgcattttc
      361 tatttttctc tctatctatt ctacttgttt attcccttca aggttttttt ttaaggagta
      421 cttgttttta gaatatacgg tcaacgaact ataattaact aaacatgtca gagttgagag
      481 ccttcagtgc cccagggaaa gcgttactag ctggtggata tttagtttta gatccgaaat
      541 atgaagcatt tgtagtcgga ttatcggcaa gaatgcatgc tgtagcccat ccttacggtt
      601 cattgcaaga gtctgataag tttgaagtgc gtgtgaaaag taaacaattt aaagatgggg
      661 agtggctgta ccatataagt cctaaaactg gcttcattcc tgtttcgata ggcggatcta
      721 agaacccttt cattgaaaaa gttatcgcta acgtatttag ctactttaag cctaacatgg
      781 acgactactg caatagaaac ttgttcgtta ttgatatttt ctctgatgat gcctaccatt
      841 ctcaggagga cagcgttacc gaacatcgtg gcaacagaag attgagtttt cattcgcaca
      901 gaattgaaga agttcccaaa acagggctgg gctcctcggc aggtttagtc acagttttaa
      961 ctacagcttt ggcctccttt tttgtatcgg acctggaaaa taatgtagac aaatatagag
     1021 aagttattca taatttatca caagttgctc attgtcaagc tcagggtaaa attggaagcg
     1081 ggtttgatgt agcggcggca gcatatggat ctatcagata tagaagattc ccacccgcat
     1141 taatctctaa tttgccagat attggaagtg ctacttacgg cagtaaactg gcgcatttgg
     1201 ttaatgaaga agactggaat ataacgatta aaagtaacca tttaccttcg ggattaactt
     1261 tatggatggg cgatattaag aatggttcag aaacagtaaa actggtccag aaggtaaaaa
     1321 attggtatga ttcgcatatg ccggaaagct tgaaaatata tacagaactc gatcatgcaa
     1381 attctagatt tatggatgga ctatctaaac tagatcgctt acacgagact catgacgatt
     1441 acagcgatca gatatttgag tctcttgaga ggaatgactg tacctgtcaa aagtatcctg
     1501 agatcacaga agttagagat gcagttgcca caattagacg ttcctttaga aaaataacta
     1561 aagaatctgg tgccgatatc gaacctcccg tacaaactag cttattggat gattgccaga
     1621 ccttaaaagg agttcttact tgcttaatac ctggtgctgg tggttatgac gccattgcag
     1681 tgattgctaa gcaagatgtt gatcttaggg ctcaaaccgc tgatgacaaa agattttcta
     1741 aggttcaatg gctggatgta actcaggctg actggggtgt taggaaagaa aaagatccgg
     1801 aaacttatct tgataaataa tagtgatcga atttacgtag cccaatctac cacttttttt
     1861 tttcattttt taaagtgtta tacttagtta tgctctagga taatgaacta cttttttttt
     1921 ttttttttta ctgttatcat aaatatatat accttattgt tgtttgcaac cgtcggttaa
     1981 ttccttatca aggttcccca agttcggatc attaccatca atttccaaca ttttcatgag
     2041 ttcttcttct tcattaccgt gttttagggg gctgttcgca cttctaatag ggctatcacc
     2101 aagctgttct aattcgtcca aaagttacta gttgtttcaa ttacgcagga cccttttacg
     2161 attgtttttc atctctagag gcattttcaa catcgtattt tccgaagcgt tgcataatga
     2221 aaatgtgata ataaggcagt gagctacttt gtcacaacaa agtacgagaa cagatgaggt
     2281 tccgtggtgt ttttggggca accgcactat ttggagcgct gtatatattt atatcctact
     2341 acaaaaaatc ataaatgaca ctgaattgga cacatgctag cgtcaaatac cttgcctggt
     2401 aaagttgtgt gctagtgtct cccgtcttct gttcactatt attcctttgg tagaccagtc
     2461 tttgcgtcaa tcaaagattc gtttgtttct tgtgggcctg aaccgacttg agttaaaatc
     2521 actctggcaa catccttttg caactcaaga tccaattcac gtgcagtaaa gttagatgat
     2581 tcaaattgat ggttgaaagc ctcaagctgc tcagtagtaa atttcttgtc ccatccagga
     2641 acagagccaa acaatttata gataaatgca aagagtttcg actcattttc agctaagtag
     2701 tacaacacag catttggacc tgcatcaaac gtgtatgcaa cgattgtttc tccgtaaaac
     2761 tgattaatgg tgtggcacca actgatgata cgcttggaag tgtcattcat gtagaatatt
     2821 ggagggaaag agtccaaaca tgtggcatgg aaagagttgg aatccatcat tgtttccttt
     2881 gcaaaggtgg cgaaatcttt ttcaacaatg gctttacgca tgacttcaaa tctctttggt
     2941 acgacatgtt caattctttc tttaaatagt tcggaggttg ccacggtcaa ttgcataccc
     3001 tgagtggaac tcacatcctt tttaatatcg ctgacaacta ggacacaagc tttcatctga
     3061 ggccagtcag agctgtctgc gatttgtact gccatggaat catgaccatc ttcagctttt
     3121 cccatttccc aggccacgta tccgccaaac aacgatctac aagctgaacc agaccccttt
     3181 cttgctattc tagatatttc tgaagttgac tgtggtaatt ggtataactt agcaattgca
     3241 gagaccaatg cagcaaagcc agcagcggag gaagctaaac cagctgctgt aggaaagtta
     3301 ttttcggaga caatgtggag tttccattga gataatgtgg gcaatgaggc gtccttcgat
     3361 tccatttcct ttcttaattg gcgtaggtcg cgcagacaat tttgagttct ttcattgtcg
     3421 atgctgtgtg gttctccatt taaccacaaa gtgtcgcgtt caaactcagg tgcagtagcc
     3481 gcagaggtca acgttctgag gtcatcttgc gataaagtca ctgatatgga cgaattggtg
     3541 ggcagattca acttcgtgtc ccttttcccc caatacttaa gggttgcgat gttgacgggt
     3601 gcggtaacgg atgctgtgta aacggtcatg tttagttaat tatagttcgt tgaccgtata
     3661 ttctaaaaac aagtactcct taaaaaaaaa ccttgaaggg aataaacaag tagaatagat
     3721 agagagaaaa atagaaaatg caagagaatt tatatattag aaagagagaa agaaaaatgg
     3781 aaaaaaaaaa ataggaaaag ccagaaatag cactagaagg agcgacacca gaaaagaagg
     3841 tgatggaacc aatttagcta tatatagtta actaccggct cgatcatctc tgcctccagc
     3901 atagtcgaag aagaattttt tttttcttga ggcttctgtc agcaactcgt attttttctt
     3961 tcttttttgg tgagcctaaa aagttcccac gttctcttgt acgacgccgt cacaaacaac
     4021 cttatgggta atttgtcgcg gtctgggtgt ataaatgtgt gggtgcaaca tgaatgtacg
     4081 gaggtagttt gctgattggc ggtctataga taccttggtt atggcgccct cacagccggc
     4141 aggggaagcg cctacgcttg acatctacta tatgtaagta tacggcccca tatatatata
     4201 tatatataca ttaaacatta ttggtaaata caccagcaac cgcatgattg atgttatggt
     4261 ggaatatagg tagctaaaaa aactctacat aacaaagtga gctcttcgtg attctcctgc
     4321 agtggaacaa gtagaggtgt attctgagga ggatgatgag tactctactg atgacgacga
     4381 aaccgaaagt gatgatgaaa gtgatgctag cgattatact gatatcgata cggatacaga
     4441 agctgaagat gatgaatcac cagaatagat ataagtgatt agtatagttt ttcattttag
     4501 ctcttagacg tatatatttc atctttataa aaatagatac atgtgtatac aaagcaaaac
     4561 caaaaaaaaa aaaaaaattt ttcagccatc tgttaagaaa tgtgtcacta ttatgaagtc
     4621 catggtaaat tcgtgtttcc tggcaataat agatcgtcaa tttgttgctt tgtggtagtt
     4681 ttattttcaa ataattggaa tactagggat ttgattttaa gatctttatt caaatttttt
     4741 gcgcttaaca aacagcagcc agtcccaccc aagtctgttt caaatgtctc gtaactaaaa
     4801 tcatcttgca atttcttttt gaaactgtca atttgctctt gagtaatgtc tcttcgtaac
     4861 aaagtcaaag agcaaccgcc gccaccagca ccggtaagtt ttgtggagcc aattctcaaa
     4921 tcatcgctca gatttttaat aagttctaat ccaggatgag aaacaccgat tgagacaagc
     4981 agtccatgat ttattcttat caattccaat agttgttcat acagttcatt attagtttct
     5041 acagcctcgt catcggtgcc tttacattta cttaacttag tcatgatctc taagccttgt
     5101 agggcacatt cacccatggc atctagaatt ggcttcataa cttcaggaaa tttctcggtg
     5161 accaacacac gaacgcgagc aacaagatct tttgtagacc ttggaattct agtataggtt
     5221 aggatcattg gaatggctgg gaaatcatct aagaacttaa aattgtttgt gtttattgtt
     5281 ccattatgtg agtctttttc aaatagcagg gcattaccat aagtggccac agcgttatct
     5341 attcctgaag gggtaccgtg aatacacttt tcacctatga aggcccattg attcactata
     5401 tgcttatcgt tttctgacag cttttccaag tcattagatc ctattaaccc ccccaagtag
     5461 gccatagcta aggccagtga tacagaaata gaggcgcttg agcccaaccc agcaccgatg
     5521 ggtaaagtag actttaaaga aaacttaata ttcttggcat gggggcatag gcaaacaaac
     5581 atatacagga aacaaaacgc tgcatggtag tggaaggatt cggatagttg agctaacaac
     5641 ggatccaaaa gactaacgag ttcctgagac aagccatcgg tggcttgttg agccttggcc
     5701 aatttttggg agtttacttg atcctcggtg atggcattga aatcattgat ggaccactta
     5761 tgattaaagc taatgtccgg gaagtccaat tcaatagtat ctggtgcaga tgactcgctt
     5821 attagcaggt aggttctcaa cgcagacaca ctagcagcga cggcaggctt gttgtacaca
     5881 gcagagtgtt caccaaaaat aataaccttt cccggtgcag aagttaagaa cggtaatgac
     5941 attttatgtg atgattgatt gattgattgt acagtttgtt tttcttaata tctatttcga
     6001 tgacttctat atgatattgc actaacaaga agatattata atgcaattga tacaagacaa
     6061 ggagttattt gcttctcttt tatatgattc tgacaatcca tattgcgttg gtagtctttt
     6121 ttgctggaac ggttcagcgg aaaagacgca tcgctctttt tgcttctaga agaaatgcca
     6181 gcaaaagaat ctcttgacag tgactgacag caaaaatgtc tttttctaac tagtaacaag
     6241 gctaagatat cagcctgaaa taaagggtgg tgaagtaata attaaatcat ccgtataaac
     6301 ctatacacat atatgaggaa aaataataca aaagtgtttt aaatacagat acatacatga
     6361 acatatgcac gtatagcgcc caaatgtcgg taatgggatc ggcttacctg caggttataa
     6421 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6481 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     6541 atctgtgcgg tatttcacac cgcatagatc ggcaagtgca caaacaatac ttaaataaat
     6601 actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt ttgttagagt
     6661 cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg ccatttaatc
     6721 taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa tgtaagcttt
     6781 cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa agttcacctg
     6841 tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt gaagctgcac
     6901 tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc aaaccgagga
     6961 actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca atgccgtaat
     7021 cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca accaagtatt
     7081 tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc cttgcaataa
     7141 ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag tcagcatcgg
     7201 aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac
     7261 cagaactacc tgtgaaatta ataacagaca tactccaagc tgcctttgtg tgcttaatca
     7321 cgtatactca cgtgctcaat agtcaccaat gccctccctc ttggccctct ccttttcttt
     7381 tttcgaccga attaattctt aatcggcaaa aaaagaaaag ctccggatca agattgtacg
     7441 taaggtgaca agctattttt caataaagaa tatcttccac tactgccatc tggcgtcata
     7501 actgcaaagt acacatatat tacgatgctg ttctattaaa tgcttcctat attatatata
     7561 tagtaatgtc gttgatctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7621 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7681 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7741 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7801 taatgtcatg ataataatgg tttcttagta tgatccaata tcaaaggaaa tgatagcatt
     7861 gaaggatgag actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc
     7921 tgaaggaagc atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta
     7981 cctttcatcc tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat
     8041 gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac
     8101 agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc
     8161 tttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt
     8221 ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt
     8281 aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa agtatctctt
     8341 tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga
     8401 acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga
     8461 caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa
     8521 catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc
     8581 aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt
     8641 atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa
     8701 tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag
     8761 tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag actgcattat
     8821 agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa tagcgctctc
     8881 gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa aatagcgctc
     8941 tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg
     9001 ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag
     9061 cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat
     9121 tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt cgttcaggtg
     9181 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9241 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9301 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9361 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9421 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9481 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9541 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9601 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9661 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9721 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9781 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9841 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9901 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9961 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10021 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10081 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10141 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10201 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10261 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10981 acatgtgcgg ccgcgtataa acaatgcata ctttgtacgt tcaaaataca atgcagtaga
    11041 tatatttatg catattacat ataatacata tcacatagga agcaacaggc gcgttggact
    11101 tttaattttc gaggaccgcg aatccttaca tcacacccaa tcccccacaa gtgatccccc
    11161 acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact
    11221 ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt tcccctcttt
    11281 cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagagaccg
    11341 cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct
    11401 tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat atttaagtta
    11461 ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat tacaactttt
    11521 tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt aattacaaaa
    11581 tgactgccga caacaatagt atgccccatg gtgcagtatc tagttacgcc aaattagtgc
    11641 aaaaccaaac acctgaagac attttggaag agtttcctga aattattcca ttacaacaaa
    11701 gacctaatac ccgatctagt gagacgtcaa atgacgaaag cggagaaaca tgtttttctg
    11761 gtcatgatga ggagcaaatt aagttaatga atgaaaattg tattgttttg gattgggacg
    11821 ataatgctat tggtgccggt accaagaaag tttgtcattt aatggaaaat attgaaaagg
    11881 gtttactaca tcgtgcattc tccgtcttta ttttcaatga acaaggtgaa ttacttttac
    11941 aacaaagagc cactgaaaaa ataactttcc ctgatctttg gactaacaca tgctgctctc
    12001 atccactatg tattgatgac gaattaggtt tgaagggtaa gctagacgat aagattaagg
    12061 gcgctattac tgcggcggtg agaaaactag atcatgaatt aggtattcca gaagatgaaa
    12121 ctaagacaag gggtaagttt cactttttaa acagaatcca ttacatggca ccaagcaatg
    12181 aaccatgggg tgaacatgaa attgattaca tcctatttta taagatcaac gctaaagaaa
    12241 acttgactgt caacccaaac gtcaatgaag ttagagactt caaatgggtt tcaccaaatg
    12301 atttgaaaac tatgtttgct gacccaagtt acaagtttac gccttggttt aagattattt
    12361 gcgagaatta cttattcaac tggtgggagc aattagatga cctttctgaa gtggaaaatg
    12421 acaggcaaat tcatagaatg ctataatagt gagctggttg atggaaaata taattttatt
    12481 gggcaaactt ttgtttatct gatgtgtttt atactattat ctttttaatt aatgattcta
    12541 tatacaaaac tgtatatttt ttctttaacc aatttttttt tatagaccta gagctgtact
    12601 tttattctgc tatcaagcaa acccctaccc cctcttctca atcctcccct caggcagaac
    12661 ttatctacct gtatcaagga gcggacgagg gagtcctagt tgttctacgt ataccaatgc
    12721 tagcagctta cataggtggt ggcactacca gtcgacactt tgttatgtag agttttttta
    12781 gctacctata ttccaccata acatcaatca tgcggttgct ggtgtattta ccaataatgt
    12841 ttaatgtata tatatatata tatatggggc cgtatactta catatagtag atgtcaagcg
    12901 taggcgcttc ccctgccggc tgtgagggcg ccataaccaa ggtatctata gaccgccaat
    12961 ca
//
