LOCUS       sequence-190211-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  47xCAG repeats in pBluescript vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Jain A, Vale RD
  TITLE     RNA phase transitions in repeat expansion disorders.
  JOURNAL   Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub
            2017 May 31.
  PUBMED    28562589
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature22386"; journalName: "Nature"; date: "2017-06-8- 8"; 
            volume: "546"; issue: "7657"; pages: "243-247"
COMMENT     Sequence Label: pBluescript-CTG-47
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(438..457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(439..457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(467..484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(467..483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      625..1080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(712..731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     922..943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1106..1210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1211..1279,1280..2071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1429..1448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2242..2830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2731..2750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2984..3001
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc tcccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggcctcc
      181 cctcgagagg ccggaagagc ctagttagct agttagctag ctaaagcttg atatcgaatt
      241 cggtctcaca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
      301 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
      361 agcagcagca gcagcagcag cagcagcaga gacggagctc gtcgacgcgg ccgccaccgc
      421 ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      601 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      841 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      961 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1021 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1081 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1141 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1201 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1261 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1321 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1381 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1441 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1501 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1561 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1621 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1681 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1741 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1801 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1861 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1921 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1981 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2041 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2101 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2161 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2221 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2281 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2341 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2401 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2461 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2521 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2581 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2641 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2701 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2761 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2821 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2881 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2941 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3001 ggaagcggaa gtgcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3061 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaat
//
